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Camptothecin and 10-hydroxycamptothecin in callus and plantlets of t Camptotheca acuminata

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Abstract

The process of callus induction, organogenesis and plantlets regeneration of Camptotheca acuminata Decne is reported. The highest growth rate of callus was observed on MS medium with 1 mg l−1 NAA, 1 mg l−1 kinetin and 60 g l−1 sucrose. All tissues and organs developed in vitro contain camptothecin and 10-hydroxycamptothecin. The presence of 10-hydroxycamptothecin in shoots and callus of Camptotheca acuminata Decne is reported for the first time. The alkaloids were detected and identified using HPLC methods.

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Authors and Affiliations

  1. Pharmazeutisches Institut der Universität Bonn, Bonn, Germany

    Helmut Wiedenfeld & Erhard Roeder

  2. Department of Biology and Pharmaceutical Botany, Medical Academy, Warsaw, Poland

    Miroslawa Furmanowa, Joanna Guzewska & Wlodzimierz Gustowski

Authors
  1. Helmut Wiedenfeld
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  2. Miroslawa Furmanowa
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  3. Erhard Roeder
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  4. Joanna Guzewska
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  5. Wlodzimierz Gustowski
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Wiedenfeld, H., Furmanowa, M., Roeder, E. et al. Camptothecin and 10-hydroxycamptothecin in callus and plantlets of t Camptotheca acuminata. Plant Cell, Tissue and Organ Culture 49, 213–218 (1997). https://doi.org/10.1023/A:1005704429339

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  • DOI: https://doi.org/10.1023/A:1005704429339

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