Abstract
We tested the value of a matrix attachment region (MAR) fragment derived from a tobacco gene for increasing the frequency of Agrobacterium-mediated transformation. A binary vector that carried a GUS reporter gene containing an intron and an nptII gene was modified to contain flanking MAR elements within the T-DNA borders. Vectors containing or lacking MARs were then used to transform tobacco, a readily transformabl e poplar clone (Populus tremula × P. alba), and a recalcitrant poplar clone (Populus trichocarpa × P. deltoides). MARs increased GUS gene expression approximately 10-fold in the two hybrid poplar clones and twofold in tobacco one month after cocultivation with Agrobacterium; MARs also increased the frequency of kanamycin-resistant poplar shoots recovered
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HAN, KH., MA, C. & STRAUSS, S.H. Matrix attachment regions (MARs) enhance transformation frequency and transgene expression in poplar. Transgenic Res 6, 415–420 (1997). https://doi.org/10.1023/A:1018439518649
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DOI: https://doi.org/10.1023/A:1018439518649