Abstract
The percentage of T and B lymphocytes in the peripheral blood of horses chronically infected with equine infectious anaemia (EIA) virus was determined and the results were compared with the percentage of these cells in healthy uninfected horses. Cells with membrane receptors for sheep erythrocytes (T and active T lymphocytes) were determined by E and A rosette techniques, while cells with receptors for the C3b component of complement and those with receptors for mouse erythrocytes (B lymphocytes), were determined by the EAC rosette method. The percentage of Fc positive cells was assayed by the EA rosette test.
The majority of peripheral blood lymphocytes (PBL) from both uninfected and EIA-infected horses formed rosettes of each kind with only three erythrocytes indicating a low density of the corresponding receptors on the cell membrane under the condition of the assays used. The percentage of T lymphocytes in the peripheral blood of diseased horses (52.4±1.6%), as detected by E rosettes, was significantly (p<0.01) higher than in control animals (42.4±3.5%). In clinically healthy horses 8.9±1.1% of PBL were identified by A rosettes as active T cells, whereas animals with a chronic form of EIA had a much lower (p<0.001) percentage of these cells (4.7±0.7%). In the B lymphocyte subpopulations the percentages of cells bearing Fc and C3b receptors were markedly elevated (p<0.001) in EIA-infected horses (24.7±0.8% and 42.8±2.2% respectively) as compared to uninfected animals (15.1±1.4% and 29.6±1.2% respectively). Receptors for mouse erythrocytes, as yet undescribed on equine PBL, were demonstrated in approximately equal proportions on lymphocytes from EIA-infected (24.8±1.5%) and uninfected horses (24.3±2.1%).
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References
Amirbekov, M.A. 1981. Cellular immune reactions in the horse: identification of T lymphocytes. Trudy Vsesoyuznogo Instituta Eksperimental'noi Veterinarii, 53, 50–54
Ayanwale, F.O. and Atluru, R. 1983. Assay of equine peripheral blood lymphocytes by erythrocyte (E) rosette formation methods. Tropical Veterinarian, 1, 25–28
Bach, J.F., Dardenne, M. and Fournier, C. 1969. In vitro evaluation of immunosuppressive drugs. Nature, 222, 998–999
Banks, K.L. 1975. Monocyte activation in horses persistently infected with equine infectious anemia virus. Infection and Immunity, 12, 1219–1221
Banks, K.L. and Henson, J.B. 1973. Quantitation of immunoglobulin-bearing lymphocytes and lymphocyte response to mitogens in horses persistently infected by equine infectious anemia virus. Infection and Immunity, 8, 679–682
Cheevers, W.P. and McGuire, T.C. 1985. Equine infectious anemia virus: immunopathogenesis and persistence. Reviews of Infectious Diseases, 7, 83–88
Cheevers, W.P., Watson, S.G. and Klevjer-Anderson, P. 1982. Persistent infection by equine infectious anemia virus: asymmetry of nucleotide sequence reiteration in the integrated provirus of persistently infected cells. Virology, 118, 246–253
Coggins, L., Matheka, H.D. and Charman, H.P. 1978. Radioimmunoassay for equine infectious anemia virus antigen and antibody. Journal of Equine Medicine and Surgery, 1, 351–358
Fujimiya, Y., Perryman, L.E. and Crawford, T.B. 1979. Leukocyte cytotoxicity in a persistent virus infection: Presence of direct cytotoxicity but absence of antibody dependent cellular cytotoxicity in horses infected with equine infectious anemia virus. Infection and Immunity, 24, 628–636
Issel, C.J. and Coggins, L. 1979. Equine infectious anemia: Current knowledge. Journal of the American Veterinary Medical Association, 174, 727–733
Kono, Y., Sentsui, H. and Murakami, Y. 1976. Development of specific in vitro lymphocyte stimulation responses in horses infected with equine infectious anemia virus. Veterinary Microbiology, 1, 31–43
Lu, C.L., He, T.T., Kung, X.G. and Lin, H.Y. 1985. Scanning electron microscopy of lymphocytes from horses vaccinated with equine infectious anaemia live vaccine. Acta Veterinaria et Zootechnica Sinica, 16, 111–114
Lu, C.L., He, T.T., Lin, H.Y., Liu, C.P. and Kung, X.G. 1981. Studies on the cell-mediated immunity of horses immunized with equine infectious anaemia live vaccine. Science in Agriculture Sinica, 5, 83–89
McGuire, T.C. and Crawford, T.B., 1978. Induction of a cell membrane antigen by equine infectious anemia virus. American Journal of Veterinary Research, 39, 385–386
McGuire, T.C., Crawford, T.B. and Henson, J.B. 1972. Equine infectious anemia: Detection of infectious virus-antibody complexes in the serum. Immunological Communications, 1, 545–551
Shen, D.T., Gorham, J.R. and McGuire, T.C. 1984. Enzyme-linked immunosorbent assay for detection of equine infectious anemia antibody to purified P 26 viral protein. American Journal of Veterinary Research, 45, 1542–1543
Shively, M.A., Banks, K.L., Greenlee, A. and Klevjer-Anderson, P. 1982. Antigenic stimulation of T lymphocytes in chronic nononcogenic retrovirus infection: equine infectious anemia. Infection and Immunity, 36, 38–46
Tarr, M.J., Olsen, R.G., Krakowka, G.S., Cockerell, G.L. and Gabel, A.A. 1977. Erythrocyte rosette formation of equine peripheral blood lymphocytes. American Journal of Veterinary Research, 38, 1775–1779
Valpotić, I., Gerenčer, M., Judić, B. and Bašić, I. 1983. In vitro reactivity of lymphocytes in horses affected by equine infectious anemia to mitogens and specific antigens. Periodicum biologorum, 85, Suppl. 3, 233–235
Vautherot, J.F. and Salmon, H. 1977. Adsorption élective des erythrocytes de cobaye sur differentes sous-populations leucocytaires du cheval. Bulletin de l'Académie Vétérinaire de France, 50, 293–302
Wybran, J. and Fudenberg, H.H. 1973. Thymus-derived rosette-forming cells in various human disease states: Cancer, lymphoma, bacterial and viral infections, and other diseases. The Journal of Clinical Investigation, 52, 1026–1032
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Valpotić, I., Kaštelan, M., Rudolf, M. et al. T and B lymphocytes in horses persistently infected with equine infectious anaemia virus. Veterinary Research Communications 13, 57–65 (1989). https://doi.org/10.1007/BF00366853
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DOI: https://doi.org/10.1007/BF00366853