Abstract
A steroid-biotransforming strain RJ6 was identified as Micrococcus roseus. This bacterium has a 10 kb plasmid pMQV10. Curing mediated through cultivation of the culture with a low concentration (200 ng/ml) of mitomycin C is described. Loss of cholesterol degradation (chol+) and streptomycin resistance (Smr) phenotypes as a consequence of the loss of plasmid indicate the extrachromosomal location of these two genes in this strain. An electroporation procedure was developed for transformation of cured strain of Micrococcus (RJC6) by plasmids. Frequency of greater than 105 transformants/μg DNA was achieved, which is 100-fold higher than the standard transformation procedure that yielded 5.3×103 transformants/μg DNA in the same strain.
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Dogra, N., Qazi, G. Biotransformation of cholesterol by Micrococcus species mediated by plasmid DNA. World Journal of Microbiology and Biotechnology 15, 411–415 (1999). https://doi.org/10.1023/A:1008990910303
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DOI: https://doi.org/10.1023/A:1008990910303