Skip to main content
Log in

Influence of amylose, amylopectin and pullulan on β-glucuronidase activity of starch-degrading Escherichia coli

  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

Synthesis of β-glucuronidase in starch-degrading Escherichia coli (S1) was induced by amylose, amylopectin and pullulan supplied in mineral medium as the sole carbon source (1%, w/v). The maximum activity occurred after 4 days when cultures reached the stationary phase of growth, but induction was also evident during log-phase. The effects obtained with amylose, amylopectin and pullulan were higher than that obtained with maize starch.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Cenci, G., Caldini, G. & Strappini, C. 1998 Effect of different starches on Escherichia coli (S1) β-glucuronidase expression. Journal of Basic Microbiology 38, 95–100.

    Google Scholar 

  • Feng, P. & Lampel, K.A. 1994 Genetic analysis of uidA expression in enterohaemorrhagic Escherichia coli serotype O157:H7. Microbiology 140, 2101–2107.

    Google Scholar 

  • Hill, M.J. 1995 Carbohydrate metabolism. In Role of Gut Bacteria in Human Toxicology and Pharmacology, ed. M.J. Hill, pp. 95–104. London: Taylor & Francis. ISBN 0-7484-0110-5.

    Google Scholar 

  • Martins, M.T., Rivera, I.G., Clark, D.L., Stewart, M.H., Wolfe, R.L. & Olson, B.H. 1993 Distribution of uidA gene sequences in Escherichia coli isolates in water sources and comparison with the expression of β-glucuronidase activity in 4-methylumbelliferil-β-D-glucuronide media. Applied and Environmental Microbiology 59, 2271–2276.

    Google Scholar 

  • Peekhaus, N. & Conway, T. 1998 What's for dinner?: Entner-Doudoro. metabolism in Escherichia coli. Journal of Bacteriology 180, 3495–3502.

    Google Scholar 

  • Tryland, I. & Fiksdal, L. 1998 Enzyme characteristics of β-D-galactosidase-and β-D-glucuronidase-positive bacteria and their interference in rapid methods for detection of waterborne coliforms and Escherichia coli. Applied and Environmental Microbiology 64, 1018–1023.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Caldini, G., Cenci, G. & Strappini, C. Influence of amylose, amylopectin and pullulan on β-glucuronidase activity of starch-degrading Escherichia coli. World Journal of Microbiology and Biotechnology 15, 497–499 (1999). https://doi.org/10.1023/A:1008992405277

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1008992405277

Navigation