Abstract
Immobilized baker's yeast entrapped in calcium alginate beads, ca 1.5 mm diameter, was used more than 10 times in water and reduced ethyl 3-oxobutanoate and ethyl benzoylformate to the corresponding chiral hydroxy esters in good chemical yields and in high enantioselectivities. The biocatalyst was also successfully used in organic/water solvent systems such as hexane/water and acetonitrile/water, and in other systems, particularly in hexane, converted keto esters into their individual chiral hydroxy esters.
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Naoshima, Y., Munakata, Y., Nishiyama, T. et al. Asymmetric bioreduction of keto esters by immobilized baker's yeast entrapped in calcium alginate beads in both water and organic/water solvent systems. World Journal of Microbiology and Biotechnology 7, 219–224 (1991). https://doi.org/10.1007/BF00328993
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DOI: https://doi.org/10.1007/BF00328993