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Formation of colonies of P-388 leukemic cells in semisolid agar

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Abstract

By alternate passages of P-388 mouse ascites leukemia cells in a primary agarized culture and intraperitoneally in mice a substrain P-388/A2 adapted to culture in agar gel in the form of compact colonies without the use of special conditions of colony-stimulating factors was obtained. Reseeding efficiency and size of the colonies depended on the original density of the cell suspension. After the addition of 100 cells/ml seeding efficiency reached 20% and the number of cells in the colony by the 8th–10th day of culture was 13,000.

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Literature Cited

  1. L. P. Ivanitskaya and L. V. Makukho, Vopr. Onkol., No. 5, 67 (1973).

    Google Scholar 

  2. L. P. Ivanitskaya, L. V. Makukho, and M. V. Bibikova, Antibiotiki, No. 2, 174 (1976).

    Google Scholar 

  3. S. I. Shereshkov, Lab. Delo, No. 3, 146 (1974).

    Google Scholar 

  4. B. Bhuyan, T. Fraser, and L. Li, Cancer Res.,32, 2538 (1972).

    Google Scholar 

  5. T. Bradly, in: Second International Workshop on Hemopoiesis in Culture, Washington (1974), pp. 424–428.

  6. W. Bruce, B. Meeker, and F. Valeriotte, J. Nat. Cancer Inst.,37, 233 (1966).

    Google Scholar 

  7. K. Dicke, in: Second International Workshop on Hemopoiesis in Culture, Washington (1974), pp. 414–424.

  8. R. Dulbecco and J. Elkington, Proc. Nat. Acad. Sci. USA,72, 1584 (1975).

    Google Scholar 

  9. H. Lazarus, E. Barell, and S. Oppenheim, In Vitro,9, 303 (1974).

    Google Scholar 

  10. M. Lippman, In Vitro,9, 370 (1974).

    Google Scholar 

  11. D. Lutz, H. Brohs, B. Haist, et al., Int. J. Clin. Pharmacol.,9, 146 (1974).

    Google Scholar 

  12. D. Metcalf, M. Moore, and N. Warner, J. Nat. Cancer Inst.,43, 982 (1969).

    Google Scholar 

  13. A. Morley and D. Higgs, Cancer Res.,34, 2307 (1974).

    Google Scholar 

  14. M. Shimoyama and K. Kimura, Gann,63, 733 (1972).

    Google Scholar 

  15. R. Todey, Cancer Res.,32, 2720 (1972).

    Google Scholar 

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Authors and Affiliations

  1. Academy of Medical Sciences of the USSR, USSR

    S. M. Navashin (Corresponding Member)

Authors
  1. L. P. Ivanitskaya
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  2. M. V. Bibikova
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  3. L. V. Egorov
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  4. S. M. Navashin
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Additional information

All-Union Research Institute of Antibiotics, Ministry of the Medical Industry of the USSR. Central Institute of Hematology and Blood Transfusion, Ministry of Health of the USSR, Moscow. Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 86, No. 7, pp. 120–122, July, 1978.

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Ivanitskaya, L.P., Bibikova, M.V., Egorov, L.V. et al. Formation of colonies of P-388 leukemic cells in semisolid agar. Bull Exp Biol Med 86, 973–975 (1978). https://doi.org/10.1007/BF00801329

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  • DOI: https://doi.org/10.1007/BF00801329

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