Abstract
These experiments have examined the physical properties and the hepatic uptake of non-transferrin-bound iron, a fraction found in the serum of untreated primary hemochromatosis patients. Ultrafiltration, polyacrylamide gel electrophoresis, and TLC have demonstrated the non-transferrin-bound iron complex to have a molecular weight<1000 and to be distinct from free iron and amino acid iron complexes. Hepatic iron uptake from serum containing non-transferrin-bound iron was significantly higher than from the same serum sample from which the abnormal iron had been removed. Non-transferrin-bound iron entering the liver was localized to the lysosomal fraction in the first hour and was subsequently incompletely incorporated into ferritin. The results demonstrated an abnormal uptake of this iron fraction by the liver and suggest that the abnormal serum fraction may contribute to tissue damage by entering the lysosomes.
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References
Fletcher J: The plasma clearance and liver uptake of iron from transferrin at low and high iron saturation. Clin Sci 41:395–402, 1971
Gardiner ME, Morgan EH: Transferrin and iron uptake by the liver in the rat. Aust J Exp Biol Med Sci 52:723–736, 1974
Beamish MR, Keay L, Okigaki T, Brown EB: Uptake of transferrin bound iron by red cells in tissue culture. Br J Haematol 31:479–491, 1975
Batey R, Gallagher ND: Effect of iron stores and hysterectomy on iron absorption and distribution in pregnant mice. Am J Physiol 232:E47-E61, 1977
Pollycove M, Fawwaz RA, Winchell HS: Transient hepatic deposition of iron in primary hemochromatosis with iron deficiency following venesection. J Nucl Med 12:28–30, 1971
Batey RG, Pettit JE, Nicholas AW, Sherlock S, Hoffbrand AV: Hepatic iron clearance from serum in treated hemochromatosis. Gastroenterology 75:856–859, 1978
Sarkar B: State of iron(III) in normal human serum: Low in molecular weight of protein ligands besides transferrin. Can J Biochem 48:1339–1350, 1970
Dern RJ, Monti A, Glynn MF: Studies with doubly labelled iron, IV. Evidence for a second iron binding system in plasma. J Lab Clin Med 61:280–291, 1963
Hosain F, Finch CA: Ferrokinetics: A study of transport iron in plasma. J Lab Clin Med 64:905–914, 1964
Hershko C, Graham G, Bates GW, Rachmilewitz EA: Non specific serum iron in thalassemia: An abnormal serum iron fraction of potential toxicity. Br J Haematol 50:255–263, 1978
Batey RG, Lai Chung Fong P, Shamir S, Sherlock S: A non-transferrin-bound serum iron in idiopathic hemochromatosis. Dig Dis Sci 25:340–346, 1980
Milsom JP, Batey RG: Mechanism of hepatic iron uptake from native and denatured transferrin and its subcellular metabolism in the liver cell. Biochem J 182:117–125, 1979
Lowry OH, Rosebrough NJ, Farr Al, Randall, RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275, 1961
Batey RG, Williams K, Milsom, JP: A physiological model for hepatic metabolism of transferrin bound iron. Am J Physiol 238:G30–33, 1980
Seymour CA, Peters TJ: Organelle pathology in primary and secondary hemochromatosis with special reference to lysosomal changes. Br J Haematol 40:239–253, 1978
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Dr. R. Batey and Miss J. Wilms were supported by a grant from the N.H. & M.R.C. of Australia. Miss S. Shamir was supported by the Bushell Trust, Sydney, Australia.
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Batey, R.G., Shamir, S. & Wilms, J. Properties and hepatic metabolism of non-transferrin-bound iron. Digest Dis Sci 26, 1084–1088 (1981). https://doi.org/10.1007/BF01295972
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DOI: https://doi.org/10.1007/BF01295972