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Phenotypic and functional characterization of T-cell lines generated from colonoscopic biopsy specimens in patients with ulcerative colitis

  • Intestinal Disorders, Inflammatory Bowel Disease, Immunology, And Microbiology
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Abstract

Intestinal T-cell lines were generated from lamina propria mononuclear cells isolated from colonoscopic biopsies in ulcerative colitis patients and controls. In both ulcerative colitis and controls, expanded cells were constituted largely by T-cell receptor αβ+, CD4+, CD45RA− (helper), and CD8+, CD11b− (cytotoxic) phenotypes. T-cell receptor Vβ gene usage was not significantly changed after cell expansion and no difference was observed between ulcerative colitis and controls. Ulcerative colitis cells, especially those derived from the patients with long-standing disease, showed significantly higher levels of cytotoxicity against the target cells, including those of colonic epithelial origin, and enhanced production of tumor necrosis factor-α and interferon-γ after short incubation with anti-CD3 antibody. Generation of T-cell lines from colonoscopic biopsy specimens may be useful for detailed functional characterization of locally infiltrating T cells in ulcerative colitis patients.

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Authors and Affiliations

  1. Departments of Laboratory Medicine, Gastroenterology and Internal Medicine, Daido Hospital, Nagoya, Japan

    Kazuo Kusugami MD, Jun-Ichi Haruta MD, Mayumi Ieda, Masataka Shinoda MD, Takafumi Ando MD, Atsuo Kuroiwa MD, Kenji Ina MD, Hitoshi Iokawa, Akira Ishihara MD, Susumu Sarai MD, Yuichiro Saito MD, Mitsune Tanimoto MD & Kimitomo Morise MD

  2. First Department of Internal Medicine, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, 466, Nagoya, Japan

    Kazuo Kusugami MD, Jun-Ichi Haruta MD, Mayumi Ieda, Masataka Shinoda MD, Takafumi Ando MD, Atsuo Kuroiwa MD, Kenji Ina MD, Hitoshi Iokawa, Akira Ishihara MD, Susumu Sarai MD, Yuichiro Saito MD, Mitsune Tanimoto MD & Kimitomo Morise MD

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  1. Kazuo Kusugami MD
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Kusugami, K., Haruta, JI., Ieda, M. et al. Phenotypic and functional characterization of T-cell lines generated from colonoscopic biopsy specimens in patients with ulcerative colitis. Digest Dis Sci 40, 198–210 (1995). https://doi.org/10.1007/BF02063967

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  • DOI: https://doi.org/10.1007/BF02063967

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