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Characterization of leukocyte chemotactic activity of bacteriocin fromStreptococcus mutans Rm-10

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Abstract

Bacteriocins have several biological activities in addition to their antibacterial effect. We investigated the chemotactic properties and mode of action of purified streptococcal bacteriocin. Bacteriocin purified from a culture supernatant ofStreptococcus mutans (S. mutans) Rm-10 induced chemotaxis of human polymorphonuclear leukocytes and monocytes. Following purification, leukocyte migration appeared in one bacteriocin fraction, and this migration was dependent on the concentration gradient in dilution ranges from 1/960 to 1/15. Chemotactic activity of the bacteriocin was heat labile and trypsin sensitive. Moreover, preincubation of bacteriocin with varying dilutions of its antiserum prepared in rabbits resulted in a significant loss of the chemotactic activity.

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Authors and Affiliations

  1. Department of Pharmacology, Osaka Dental University, 5-31, Otemae 1-chome Chuo-ku, 540, Osaka, Japan

    Yoshio Tsukamoto, Sachiko Fukutani, Yasuyuki Takayama & Masakazu Mori

  2. Department of Bacteriology, Osaka Dental University, 5-31, Otemae 1-chome Chuo-ku, 540, Osaka, Japan

    Hisanori Fukushima & Hirosuke Sagawa

Authors
  1. Yoshio Tsukamoto
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  2. Sachiko Fukutani
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  3. Yasuyuki Takayama
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  4. Hisanori Fukushima
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  5. Hirosuke Sagawa
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  6. Masakazu Mori
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Tsukamoto, Y., Fukutani, S., Takayama, Y. et al. Characterization of leukocyte chemotactic activity of bacteriocin fromStreptococcus mutans Rm-10. Inflammation 14, 561–569 (1990). https://doi.org/10.1007/BF00914276

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