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Chemoattractant-induced NADPH oxidase activity in human monocytes is terminated without any association of receptor-ligand complex to cytoskeleton

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Abstract

When the chemotactic peptide formylmethionyl-leucyl-phenylalanine binds to its cell surface receptor, a transmembrane signal is generated that activates the superoxide-producing NADPH oxidase of human phagocytes. Comparing monocytes and neutrophils with regard to the production of superoxide anion induced by the peptide, we found a similar time-course for both types of cells. In neutrophils, ligand binding induced a conversion of the receptor to a high-affinity form, a change suggested to be due to an association of the receptor-ligand complex to the Triton X-100-insoluble cytoskeleton. This event has been hypothesized to terminate the signal that activates the NADPH oxidase and thereby results in cessation of the cellular production of superoxide anion. Neutrophils preincubated with the cytoskeleton-disrupting drug cytochalasin B showed an increased and prolonged superoxide anion production after activation with the peptide, thus indicating that the cytoskeleton is involved in terminating this response. Formylmethionyl-leucyl-phenylalanine was also found to induce polymerization of actin in monocytes; however, cytochalasin B had no effect on the peptide-induced generation of superoxide anion in these cells. Furthermore, also in monocytes, ligand binding induced a conversion of the receptor to a high-affinity form; however, the receptor-ligand complex did not coisolate with the Triton X-100-insoluble cytoskeleton. These results indicate that, in monocytes, the NADPH oxidase activating pathway is terminated without any association of the receptor-ligand complex to the Triton X-100-insoluble cytoskeleton.

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Authors and Affiliations

  1. Department of Medical Microbiology Faculty of Health Sciences, Linköping University, S-581 85, Linköping, Sweden

    Agneta Johansson, Eva Särndahl & Torbjörn Bengtsson

  2. Department of Cell Biology Faculty of Health Sciences, Linköping University, S-581 85, Linköping, Sweden

    Tommy Andersson

  3. Phagocyte Research Laboratory Department of Medical Microbiology and Immunology, University of Göteborg, S-413 46, Göteborg, Sweden

    Helen Lundqvist & Claes Dahlgren

Authors
  1. Agneta Johansson
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  2. Eva Särndahl
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  4. Torbjörn Bengtsson
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  5. Helen Lundqvist
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  6. Claes Dahlgren
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Johansson, A., Särndahl, E., Andersson, T. et al. Chemoattractant-induced NADPH oxidase activity in human monocytes is terminated without any association of receptor-ligand complex to cytoskeleton. Inflammation 19, 179–191 (1995). https://doi.org/10.1007/BF01534460

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