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Effect of leukocyte hydrolases on bacteria

IX. The release of lipoteichoic acid from group A streptococci and fromStrep, mutans by leukocyte extracts and by lysozyme: Relation to tissue damage in inflammatory sites

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Abstract

Human leukocyte extracts, egg white lysozyme, cationic proteins, polymyxin, colimycin, and phenol are capable of releasing lipoteichoic acids (LTA) from group A streptococci andStrep, mutans. While the extraction of LTA by phenol is optimal at pH 4.7, the release of LTA from streptococci by the other agents is optimal at pH 7.4. LTA released by all agents was found to have the same sensitizing abilities, as determined by passive hemagglutination, and to have a similar chemical composition, as shown by thin-layer chromatography and radioactive scanning. The LTA-releasing capacity of all the agents is strongly inhibited by normal human serum. The possible role played by LTA released by leukocyte factors in the pathogenesis of tissue damage during bacterial infections is discussed.

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Authors and Affiliations

  1. Department of Oral Biology, Hebrew University-Hadassah Faculty of Dental Medicine, Founded by the Alpha Omega Fraternity, Jerusalem, Israel

    Michael N. Sela, Meir Lahav & Isaac Ginsburg

Authors
  1. Michael N. Sela
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  2. Meir Lahav
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  3. Isaac Ginsburg
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Additional information

This investigation was supported by grants from the Joint Research Fund of the Hebrew University-Hadassah Faculty of Dental Medicine, founded by the Alpha Omega Fraternity, and the Hadassah Medical Organization; by grants from the Chief Scientist; the Ministry of Health, Government of Israel; and the Max Bogen Research Fund, obtained through the Friends of the Hebrew University in the United States.

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Sela, M.N., Lahav, M. & Ginsburg, I. Effect of leukocyte hydrolases on bacteria. Inflammation 2, 151–164 (1977). https://doi.org/10.1007/BF00918677

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