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Cellular immune response of patients with uveitis to peptide M, a retinal S-antigen fragment

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Abstract

Peptide M, an 18-amino acid fragment from position 303 to position 320 of retinal S-antigen, produces experimental autoimmune uveitis (EAU), similar to that produced by native S-antigen, in several vertebrate species including nonhuman primates. It was observed that 12 of the 39 (30.7%) patients with uveitis, 1 of the 29 (3.4%) patients with systemic connective tissue disorders (CTD) without eye involvement, 2 of the 7 (5.8%) patients of CTD with uveitis, 1 of the 17 (5.8%) patients with diabetic retinopathy, and none of the 19 normal healthy controls showed a significant lymphoproliferative response to peptide M (stimulation index of 3 or more). Yeast histone H3 peptide gave a positive response in 1 (2.5%), 2 (6.8%), 1 (14.2%), 2 (11.7%), and 2 (10.5%) individuals, respectively, in the different groups studied. In a few cases a positive response to yeast histone H3 peptide was observed without significant stimulation to peptide M. These findings indicate that peptide M could also be an immunogenic epitope of S-antigen in humans and be aetiopathologically related to uveitis in a subset of patients with this disease. However, unlike experimental animals, the responses to peptide M and yeast histone H3 were nonconcordant, necessitating further studies.

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Authors and Affiliations

  1. Department of Immunology, Sanjay Gandhi Post-Graduate Institute of Medical Sciences, Raebareli Road, Post Box No. 375, 226 001, Lucknow, India

    Soniya Nityanand, V. K. Singh & S. S. Agarwal

  2. Molecular Biology Section, Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, NIH, 20892, Bethesda, Maryland

    T. Shinohara

  3. Sitapur Eye Hospital, Sitapur, India

    A. K. Paul

  4. Department of Ophthalmology, King George's Medical College, 226 003, Lucknow, India

    V. Singh & P. K. Agarwal

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  1. Soniya Nityanand
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Nityanand, S., Singh, V.K., Shinohara, T. et al. Cellular immune response of patients with uveitis to peptide M, a retinal S-antigen fragment. J Clin Immunol 13, 352–358 (1993). https://doi.org/10.1007/BF00920244

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