Abstract
We developed a solid-phase radioimmunoassay with a reference standard pseudomonas antigen and used this with125I-labeled anti-human immunoglobulin to evaluate specific antibodies toPseudomonas aeruginosa, qualitatively and quantitatively, in sera from children with cystic fibrosis (CF) whose lungs were colonized by this bacterium. The results of this IgG assay correlated with the number of precipitin antibodies to the standard reference antigen determined by cross-immunoelectrophoresis in the same sera. Forced expiratory volume (FEV1; percentage predicted), determined as an indicator of lung injury in CF, was evaluated as an immunologic response to pseudomonas, against a profile derived from combined serial data on both the circulating immune complexes (CIC) and thePs. aeruginosa antibodies (N=25 CF patients; 108 sera). This revealed that in CF patients who had no specific IgG antibodies toPs. aeruginosa and no IgG-CIC had the best pulmonary function (FEV1=15±14.52%) and those with high levels of antibodies to this organism and high IgG-CIC levels had the poorest lung function (FEV1=69.75±10.99%) (P<0.05). We believe that this indicates an immunologic basis for lung injury in cystic fibrosis.
Similar content being viewed by others
References
Wood RE, Boat TF, Doershuk CF: Cystic fibrosis. Am Rev Resp Dis 113:833–878, 1976
di Sant'Agnese PA, Davis PB: Research in cystic fibrosis. N Engl J Med 295:481–485, 534–541, 597–602, 1976
Doggett RG, Harrison GM:Pseudomonas aeruginosa: Immune status in patients with cystic fibrosis. Infect Immun 6:628–635, 1972
Høiby N:Pseudomonas aeruginosa infection in cystic fibrosis. Diagnostic and prognostic significance ofPseudomonas aeruginosa precipitins determined by means of crossed immunoelectrophoresis. A survey. Acta Pathol Microbiol Scand Sect C Suppl 262:3–96, 1977
Schiøtz PO, Høiby N, Juhl F, Permin H, Nielsen H, Svehag SE: Immune complexes in cystic fibrosis. Acta Pathol Microbiol Scand Sect C 85:57–64, 1977
Schiøtz PO, Nielsen H, Høiby N, Glikmann G, Svehag SE: Immune complexes in the sputum of patients with cystic fibrosis suffering from chronicPseudominas aeruginosa lung infection. Acta Pathol Microbiol Scand Sect C 86:37–40, 1978
McFarlane H, Holzel A, Brenchley P, Allan JD, Wallwork JC, Singer BE, Worsley B: Immune complexes in cystic fibrosis. Br Med J 1:423–428, 1975
Berdischewsky M, Pollack M, Young LS, Chia D, Osher AB, Barnett EV: Circulating immune complexes in cystic fibrosis. Pediat Res 14:830–833, 1980
Moss RB, Lewiston NJ: Immune complexes and humoral response toPseudomonas aeruginosa in cystic fibrosis. Am Rev Resp Dis 121:23–29, 1980
Church JA, Jordan SC, Keens TG, Wang GI: Circulating immune complexes in patients with cystic fibrosis. Chest 80:405–411, 1981
Moss RB, Hsu YP, Lewiston NJ:125I-Clq-binding and specific antibodies as indicators of pulmonary disease activity in cystic fibrosis. J Pediat 99:215–222, 1981
Manthei U, Taussig LM, Beckerman RC, Strunk RC: Circulating immune complexes in cystic fibrosis. Am Rev Resp Dis 126:253–257, 1982
Pitcher-Wilmott RW, Levinsky RJ, Matthew DJ: Circulating soluble immune complexes containing pseudomonas antigens in cystic fibrosis. Arch Dis Child 57:577–581, 1982
Hodson ME, Beldon I, Batten JC: Circulating immune complexes in patients with cystic fibrosis in relation to clinical features. Clin Allergy 15:363–370, 1985
Moss RB, Hsu YP: Isolation and characterization of circulating immune complexes in cystic fibrosis. Clin Exp Immunol 47:301–308, 1982
Lam J, Chan R, Lam K, Costerton JW: Production of mucoid microcolonies byPseudomonas aeruginosa within infected lungs in cystic fibrosis. Infect Immun 28:546–556, 1980
Dreisin RB: Lung diseases associated with immune complexes. Am Rev Resp Dis 124:738–755, 1981
Schiøtz PO: Local humoral immunity and immune reactions in the lungs of patients with cystic fibrosis. Acta Pathol Microbiol Scand Sect C Suppl 276:1–25, 1981
Klinger JD, Straus DC, Hilton CB, Bass JA: Antibodies to proteases and exotoxin A ofPseudomonas aeruginosa in patients with cystic fibrosis: Demonstration by radioimmunoassay. J Infect Dis 138:49–58, 1978
Döring G, Høiby N: Longitudinal study of immune response toPseudomonas aeruginosa antigens in cystic fibrosis. Infect Immun 42:197–201, 1983
Matthews WJ Jr, Williams M, Oliphint B, Geha R, Colten HR: Hypogammaglobulinemia in patients with cystic fibrosis. N Engl J Med 302:245–249, 1980
Turner MW, Warner JO, Stokes CR, Norman AP: Immunological studies in cystic fibrosis. Arch Dis Child 53:631–638, 1978
Theofilopoulos AN, Wilson CB, Dixon FJ: The Raji cell radioimmune assay for detecting immune complexes in human sera. J Clin Invest 57:169–182, 1976
Dasgupta MK, Kovithavongs T, Schault J, Longenecker BM, Dossetor JB: Antibody-mediated cellular cytotoxicity against Raji cells ADCC(RAJI): Evaluation of false positives in the detection of circulating immune complexes by Raji-cell assay. J Clin Immunol 2:197–204, 1982
Lam JS, Mutharia LM, Hancock REW, Høiby N, Lam K, Baek L. Costerton JW: Immunogenicity ofPseudomonas aeruginosa outer membrane antigens examined by crossed immunoelectrophoresis. Infect Immun 42:88–98, 1983
Snedecor GW, Cochran WG: Statistical Methods, 6th ed. Ames, Iowa State University Press, 1967, p 271
Høiby N:Pseudomonas aeruginosa infection in cystic fibrosis. Relationship between mucoid strains ofPseudomonas aeruginosa and the humoral immune response. Acta Pathol Microbiol Scand Sect B 82:551–558, 1974
Theofilopoulos AN, Dixon FJ: The biology and detection of immune complexes. Adv Immunol 28:89–220, 1979
Auerbach HS, Williams M, Kirkpatrick JA, Colten HR: Alternate-day prednisone reduces morbidity and improves pulmonary function in cystic fibrosis. Lancet 2:686–688, 1985
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dasgupta, M.K., Zuberbuhler, P., Abbi, A. et al. Combined evaluation of circulating immune complexes and antibodies toPseudomonas aeruginosa as an immunologic profile in relation to pulmonary function in cystic fibrosis. J Clin Immunol 7, 51–58 (1987). https://doi.org/10.1007/BF00915425
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00915425