Abstract
The ryanodine-sensitive intracellular Ca2+ stores are known to play a major role in excitation-contraction coupling in muscles. Although these stores are also abundantly present in central neurons, their functional role in these cells remains unclear. Using fluorometric digital imaging of the intracellular Ca2+ concentration ([Ca2+] i ) in rat hippocampal slices, we investigated the dynamic properties of the ryanodine-sensitive Ca2+ stores inCA1 hippocampal pyramidal cells. We found that at rest the ryanodine-sensitive Ca2+ stores are functioning predominantly as a “sink” for Ca ions responding to an increase in [Ca2+] i with an increase in the amount of Ca ions accumulated inside the stores. If, however, [Ca2+] i increases significantly, as happens during strong neuronal discharges, the ryanodine-sensitive Ca2+ stores respond with Ca2+ release, thus acting as an amplifier of the intracellular Ca2+ signal.
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Garaschuk, O., Konnerth, A. Dual role of ryanodine-sensitive calcium stores in central neurons. Neurophysiology 29, 199–204 (1997). https://doi.org/10.1007/BF02461229
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DOI: https://doi.org/10.1007/BF02461229