Abstract
Chromatography of brain and liver 100,000g supernatants over HPLC molecular sieve columns revealed striking differences in the molecular weight distribution of ATP-sulfurylase and APS-kinase of the two tissues, pointing to different enzymic species for both enzymes in brain and liver. This was further substantiated by kinetic characterization of the two enzymes of both tissues. APS-kinase of liver is allosterically activated by ATP, while the brain enzyme is not. ATP-sulfurylase of brain is activated at high, but still physiological concentrations of ATP. Brain ATP-sulfurylase is inhibited by phenylalanine.
Similar content being viewed by others
References
Levi, A. S., and Wolfe, G. 1969. Purification and properties of the enzyme ATP-sulfurylase and its relation to vitamin A. Biochim. Biophys. Acta 178:262–282.
Burnell, J. N., and Ray, A. B. 1978. Purification and properties of the ATP-sulfurylase of rat liver. Biochim. Biophys. Acta 572:239–248.
Shoyab, M., Su, L. Y., and Marx, W. 1972. Purification and properties of ATP-sulfurylase from Furth mouse mastocytoma. Biochim. Biophys. Acta 258:113–124.
Suguhara, K., and Schwartz, N. B. 1982. Defect in 3′-phosphoadenosine-5′-phosphosulfate synthesis in brachymorphic mice. I. Characterization of the defect. Arch. Biochem. Biophys. 214:589–601.
Suguhara, K., and Schwartz, N. B. 1982. Defect in 3′-phosphoadenosine 5′-phosphosulfate synthesis in brachymorphic mice. II. Tissue distribution of the defect. Arch. Biochem. Biophys. 214:602–609.
Hommes, F. A. 1985. Myelin turnover at later stages of brain development in experimental hyperphenylalaninemia. Pages 67–85, in Bickel, H., and Wachtel, U. (eds.), Inherited diseases of amino acid metabolism. Recent progress in the understanding, recognition and management. Thieme, Stuttgart.
Hommes, F. A., and Moss, L. 1986. The assay of ATP-sulfurylase. Anal. Biochem. 154:100–104.
Robbins, P. W. 1962. Sulfate activating enzymes. Meth. Enzymol. 5:964–977.
Gregory, J. D. 1962. Sulfate transferring enzymes. Meth. Enzymol. 5:977–983.
Lowry, O. H., Rosebrough, N., Farr, L. A., and Randall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.
Shoyab, M., and Marx, W. 1972. Enzyme substrate complexes of ATP-sulfurylase from mouse mastocytoma. Biochim. Biophys. Acta 258:125–132.
Lowry, O. H., Passoneau, J. V., Hasselberger, F. X., and Schulz, D. W. 1964. Effect of ischemia on known substrates and cofactors of the glycolytic pathway in brain. J. Biol. Chem. 239:18–30.
Gaull, G. E., Tallan, H. H., Lajtha, A., and Rassin, D. K. 1975. Pathogenesis of brain dysfunction in inborn errors of amino acid metabolism. Pages 47–143 in Gaull, G. E. (ed.), Biology of brain development. Plenum, New York, Vol. 1.
Chase, H. P., and O'Brien, D. 1970. Effect of excess phenylalanine and of other amino acids on brain development in the infant rat. Pediatr. Res. 4:96–102.
London, Y., Demel, R. A., Geurts van Kessel, W. S. M., Vossenberg, F. G. A., and Van Deenen, L. L. M. 1973. The protection of A1 myelin basic protein against the action of proteolytic enzymes after interaction of the protein with lipids at the air water interface. Biochim. Biophys. Acta 311:520–530.
London, Y., and Vossenberg, F. G. A. 1973. Specific interactions of central nervous system myelin basic protein with lipids. Specific regions of the protein action of trypsin. Biochim. Biophys. Acta. 307:478–490.
Berger, R., Springer, J., and Hommes, F. A. 1980. Brain protein and myelin metabolism in young hyperphenylalaninemic rats. Mol. Cell Biol. 26:31–36.
Hommes, F. A., Eller, A. G., and Taylor, E. H. 1982. The effect of phenylalanine on myelin metabolism in adolescent rats. Pages 193–199, in Cockburn, F. and Gitzelmann, R. (eds.). Inborn errors of metabolism in humans, MTP Press, Lancaster.
Hommes, F. A., Eller, A. G., and Taylor, E. H. 1982. Turnover of the fast component of myelin and myelin proteins in experimental hyperphenylalaninemia. Relevance to termination of dietary treatment. J. Inher. Metab. Dis. 5:21–27.
Taylor, E. H., and Hommes, F. A. 1983. Effect of experimental hyperphenylalaninemia on myelin metabolism at later stages of brain maturation. Int. J. Neurosci. 20:257–228.
Matsuo, K., Moss, L., and Hommes, F. A. 1986. The development of ATP-sulfurylase and APS-kinase in rat cerebrum and liver. Submitted for publication.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Matsuo, K., Moss, L. & Hommes, F.A. Properties of the 3′-phosphoadenosine-5′-phosphosulfate (PAPS) synthesizing systems of brain and liver. Neurochem Res 12, 345–349 (1987). https://doi.org/10.1007/BF00993243
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00993243