Abstract
Mice with the dysmyelinating mutation shiverer were studied by measuring the activity of two protein methylases and myelin marker enzymes in the brain. It was observed thatS-adenosylmethionine: protein-lysineN-methyltransferase (protein methylase III, EC. 2.1.1.43) activity is significantly reduced in phenotypically affected homozygous shiverer (shi/shi) mutant mouse brain compared to the unaffected heterozygous littermate brain. This reduction in enzyme activity is manifested mainly by reduced formation of trimethyllysine during the in vitro methylation of histone. In contrast, myelin marker enzymes such as 2′,3′-cyclic nucleotide 3′-phosphohydrolase and 5′-nucleotidase as well asS-adenosyl-methionine: protein-carboxylO-methyltransferase (protein methylase II, EC. 2.1.1.24) activities were not significantly affected in these strains of mice.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bird, T. D., Farrell, D. F., and Sumi, S. M. 1978. Brain lipid composition of the shiverer mouse: genetic defect in myelin development. J. Neurochem. 331:387–391.
Privat, A., Jacque, C., Bourre, J. M., Dupouey, P., and Baumann, N. 1979. Absence of the major dense line in myelin of the mutant mouse ‘shiverer’. Neurosci. Lett. 12:107–112.
Dupouey, P., Jacque, C., Bourre, J. M., Cesselin, F., Privat, A., and Baumann, N. 1979. Immunochemical studies of myelin basic protein in shiverer mouse devoid of major dense line of myelin. Neurosci. Lett. 12:113–118.
Mikoshiba, K., Aoki, E., and Tsukada, Y. 1980. 2′3′ cyclic nucleotide 3′ phosphohydrolase activity in the central nervous system of a myelin deficient mutant (shiverer). Brain Res. 192:195–201.
Mikoshiba, K., Nagaike, K., and Tsukada, Y. 1980. Subcellular distribution and developmental change of 2′3′ cyclic nucleotide 3′ phosphohydrolase in the central nervous system of the myelin-deficient shiverer mutant mice. J Neurochem. 35:465–470.
Sidman, R. L., Conover, C. S., and Carson, J. H. 1985. Shivered gene maps near the distal end of chromosome 18 in the house mouse. Cytogenet. Cell Genet. 39:241–245.
Roach, A., Takahashi, N., Pravtcheva, D., Ruddle, F., and Hood, L. 1985. Chromosomal mapping of mouse myelin basic protein gene and structure and transcription of the partially deleted gene in shivered mutant mice. Cell 42:149–155.
Baldwin, G. S., and Carnegie, P. R. 1971. Speciffic enzymatic methylation of an arginine in the experimental allergic encephalomyelitis protein from human myelin. Science 171:579–580.
Brostoff, S., and Eylar, E. H. 1971. Localization of methylated arginine in the A1 protein from myelin. Proc. Natl. Acad. Sci. USA 68:765–769.
Crang, A. J., and Jacobson, W. 1982. The relationship of myelin basic protein (arginine) methyltransferase to myelination in mouse spinal cord. J. Neurochem. 39:244–247.
Amur, S. G., Shanker, G., and Pieringer, R. A. 1984. Regulation of myelin basic protein (arginine) methyltransferase by thyroid hormone in myelinogenic cultures of cells dissociated from embryonic mouse brain. J. Neurochem. 43:494–498.
Kim, S., Tuck, M., Kim, M., Campagnoni, A. T., and Paik, W. K. 1984. Studies on myelin basic protein methylase I in various dysmyelinating mutant mice. Biochem. Biophys. Res. Comm. 123:468–474.
Kim, S., Tuck, M., Ho, L. L., Campagnoni, A. T., Barbarese, E., Knobler, R. L., Lublin, F. D. Chanderkar, L. P., and Paik, W. K. 1986. Myelin basic protein-specific protein methylase I activity in shiverer mutant mouse brain. J. Neurosci. Res., 16:357–365.
Lowry, O. H., Rosenbrough, N. J., Farr, A. L., and Randall, R. J. 1951. Protein determination with the Folin phenol reagent. J. Biol. Chem. 93:265–275.
Paik, W. K., and Kim, S. 1970. Solubilization and partial purification of protein methylase III from calf thymus nuclei. J. Biol. Chem. 245:6010–6015.
Tuck, M., Farooqui, J. Z., and Paik, W. K. 1985. Two histone H1-specific protein lysineN-methyltransferases fromEnglena gracilis. Purification and characterization. J. Biol. Chem. 260:7114–7121.
Kim, S. 1984.S-Adenosylmethionine: protein carboxyl O-methyltransferase (protein methylase II). Pages 295–309, Vol. 16, in Methods in Enzymology, Academic Press, New York.
Prohaska, J. R., Clark, D. A., and Wells, W. E. 1973. Improved rapidity and precision in the determination of brain 2′3′-cyclic nucleotide 3′ phosphohydrolase. Anal. Biochem. 56:275–282.
Cammer, W., Sirota, S. R., Zimmerman, T. R., and Norton, W. T. 1980. 5′-Nucleotidase in rat brain myelin. J. Neurochem. 35:367–373.
Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head bacteriophage T4. Nature 227:680–685.
Kim, S., Paik, W. K., Choi, J., Lotlikar, P. D., and Magee, P. 1981. Microsome-dependent methylation of erythrocyte protein by dimethylnitrosamine. Carcinogenesis 2:179–182.
Paik, W. K., and Kim, S. 1975. Protein methylation. Pages 227–286, Vol. 42, in Meister, A. (ed.). Advances in Enzymology, John Wiley and Sons, New York.
Paik, W. K., and Kim, S. 1972. Protein methylation during the development of rat brain. Biochem. Biophys. Res. Comm. 46:933–941.
Paik, W. K., and Kim, S. 1973. Protein methylases during the development of rat brain. Biochim. Biophys. Acta 313:181–189.
Hnilica, L. S. 1972. Chemical properties of histones, Pages 3–34, in Hnilica, L. S. (ed.), The structure and biological function of histones, CRC Press, Ohio.
Paik, W. K., and Kim, S. 1980. Enzymology of protein methylation. Pages 112–141, Vol. 1, in Meister, A. (ed.). Protein Methylation, Biochemistry: A Series of Monographs, John Wiley and Sons, New York.
Paik, W. K., and Kim, S. 1985. Protein methylation. Pages 187–288, Vol. 2, in Freedman, R. B., and Hawkins, H. C. (eds.). The Enzymology of Post-Translational Modification of Proteins, Academic Press, London.
Paik, W. K., Kim, S., and Lee, H. W. 1985. Enzymology of protein methylation: recent development. Pages 15–23 in Borchardt, R. T., Creveling, C. R., and Ueland, P. M. (eds.). Biological Methylation and Drug Design, The Human Press Inc., Clifton, New Jersey.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chanderkar, L.P., Shanker, G., Knobler, R.L. et al. ReducedS-adenosylmethionine: Protein-lysineN-methyltransferase activity (protein methylase III) in shiverer mutant mouse brain. Neurochem Res 12, 445–449 (1987). https://doi.org/10.1007/BF00972296
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00972296