Abstract
The genes encoding the two plasminogen activators, tissue plasminogen activator and urokinase, were mapped to mouse chromosomes using probes derived from the respective mouse cDNAs. DNA from mouse-Chinese hamster and mouse-rat somatic cell hybrids was digested with BamHI and EcoRI, respectively, and analyzed by Southern blot hybridization for the segregation of the two genes. Tissue plasminogen activator and urokinase cosegregated with mouse chromosomes 8 and 14, respectively. The plasminogen activator genes thus fall into two syntenic groups that are conserved in human and mouse.
Literature cited
Reich, E., Rifkin, D.B., and Shaw, E. (1975).Proteases and Biological Control. (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York).
Dano, K., Andreasen, P.A., Grondahl-Hansen, J., Kristensen, P., Nielsen, L.S., and Skriver, L. (1985).Adv. Cancer Res. 44:139–266.
Pohl, G., KÄllström, M., Bergsdorf, N., Wallen, P., and Jörnvall, H. (1984).Biochemistry 23:3701–3707.
Günzler, W.A., Steffens, G.J., ötting, F., Kim, S.-M.A., Frankus, E., and Flohé, L. (1982).Hoppe-Seyler's Z. Physiol. Chem. 363:1155–1165.
Steffens, G.J., Günzler, W.A., ötting, F., Frankus, E., and Flohe, L. (1982).Hoppe-Seyler's Z. Physiol. Chem. 363:1043–1058.
Pennica, D., Holmes, W.E., Kohr, W.J., Harkins, R.N., Vehar, G.A., Ward, C.A., Bennett, W.F., Yelverton, E., Seeburg, P.H., Heyneker, H.L., Goeddel, D.V., and Collen, D. (1983).Nature 301:214–221.
Holmes, W.E., Pennica, D., Blaber, M., Rey, W.W., Günzler, W.A., Steffens, G.J., and Heyneker, H.L. (1985).Biotechnology 3:923–929.
Nagamine, Y., Pearson, D., Altus, M.S., and Reich, E. (1984).Nucleic Acids Res. 12:9525–9541.
Belin, D., Vassalli, J.-D., Combépine, C., Godeau, F., Nagamine, Y., Reich, E., Kocher, H.P., and Duvoisin, R.M. (1985).Eur. J. Biochem. 148:225–232.
Ny, T., Elgh, F., and Lund, B. (1984).Proc. Natl. Acad. Sci. U.S.A. 81:5355–5359.
Degen-Friezner, S.J., Rajput, B., and Reich, E. (1986).J. Biol. Chem. 261:6972–6985.
Riccio, A., Grimaldi, G., Verde, P., Sebastio, G., Boast, S., and Blasi, F. (1985).Nucleic acids Res. 13:2759–2771.
Rajput, B., Degen, S.F., Reich, E., Waller, E.K., Axelrod, J., Eddy, R.L., and Shows, T.B. (1985).Science 230:672–674.
Minna, J.D., Marshall, T.H., and Shaffer-Berrman, P.V. (1975).Somat. Cell Genet. 1:355–369.
Francke, U., Lalley, P.A., Moss, W., Ivy, J., and Minna, J.D. (1977).Cytogenet. Cell Genet. 19:57–84.
Lalley, P.A., Francke, U., and Minna, J.D. (1978).Proc. Natl. Acad. Sci. U.S.A. 75:2382–2386.
Killary, A.M., and Fournier, R.E.K. (1984).Cell 38:523–534.
Peterson, T.C., Killary, A.M., and Fournier, R.E.K. (1985).Mol. Cell Biol. 5:2491–2494.
Feinberg, A.P., and Vogelstein, B. (1983).Anal. Biochem. 132:6–13.
Naylor, S.L., Sakaguchi, A.Y., Shows, T.B., Lau, M.L., Goeddel, D.V., and Gray, P.W. (1983).J. Exp. Med. 157:1020–1027.
Southern, E.M. (1975).J. Mol. Biol. 98:503–517.
PolgÄr, L., and Halász, P. (1982).Biochem. J. 207:1–10.
de HaËn, C., Neurath, H., and Teller, D.C. (1975).J. Mol. Biol. 92:255–259.
Neurath, H., Walsh, K.A., and Winter, W.P. (1967).Science 158:1638–1644.
Shows, T.B., McAlpine, P.J., and Miller, R.L. (1984).Cytogenet. Cell Genet. 37:340–393.
Honey, N.K., Sakaguchi, A.Y., Quinto, C., Mac-Donald, R.J., Bell, G.I., Craik, C., Rutter, W.J., and Naylor, S.L. (1984).Somat. Cell Genet. 10:369–376.
Honey, N.K., Sakaguchi, A.Y., Quinto, C., Mac-Donald, R.J., Rutter, W.J., and Naylor, S.L. (1984).Cytogenet. Cell Genet. 37:492.
Honey, N.K., Sakaguchi, A.Y., Lalley, P.A., Quinto, C., MacDonald, R.J., Craik, C., Bell, G.I., Rutter, W.J., and Naylor, S.L. (1984).Somat. Cell Genet. 10:377–383.
McKusick, V.A. (1984).Clin. Genet. 25:89–123.
Azen, E.A., Carlson, D.M., Clements, S., Lalley, P.A., and Vanin, E. (1984).Science 226:967–969.
Lalley, P.A., and McKusick, V.A. (1985).Cytogenet. Cell Genet. 40:536–566.
Lalley, P.A., Minna, J.D., and Francke, U. (1978).Nature 274:160–163.
Yang-Feng, T.L., Opdenakker, G., Volckaert, G., and Francke, U. (1986).Am. J. Hum. Genet. 39:79–87.
Samuelson, L.C., and Farber, R.A. (1985).Somat. Cell Genet. 11:157–165.
Tripputi, P., Blasi, F., Verde, P., Cannizzaro, L.A., Emanual, B.S., and Croce, C.M. (1985).Proc. Natl. Acad. Sci. U.S.A. 82:4448–4452.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rajput, B., Marshall, A., Killary, A.M. et al. Chromosomal assignments of genes for tissue plasminogen activator and urokinase in mouse. Somat Cell Mol Genet 13, 581–586 (1987). https://doi.org/10.1007/BF01534500
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01534500