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Möglichkeiten zur gezielten Diagnose durch Bestimmung der sauren Desoxyribonucleasen im Urin gesunder Kontrollpersonen und Xeroderma pigmentosum-Patienten

Possibilities of directed diagnosis. Assessment of acid desoxyribonucleases in urine of healthy controls and xeroderma pigmentosum patients: Means for specific diagnosis

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Summary

The distribution pattern of desoxyribonuclease (DNase) activity1 in the urine of healthy subjects and xeroderma pigmentosum (X.p.) patients has been examined by micro disc electrophoresis. When incubated at pH 5.0, four distinct bands of DNase activity are shown in normal urine. In urine of X.p. patients a significant decrease of the second to fourth band can be observed. The change of the third band is extremely obvious. Further to the influence of native DNA, denaturated DNA and EDTA on the pattern of DNase activities were studied. The decrease of DNase activity in X. p. urine is not due to the presence of an inhibitor but is rather caused by lower enzyme concentrations.

Zusammenfassung

Das Verteilungsmuster der DNase-Aktivität1 im Urin von Normalpersonen und X.p.-Patienten wurde mit einem mikro-disk-elektrophoretischen Verfahren untersucht. Bei saurer Inkubation sind 4 distinkte Aktivitätsbanden im Normalurin nachweisbar. Urin von X.p.-Patienten zeigt eine deutliche Verminderung der 2.–4. Bande, wobei die Veränderung der 3. Bande besonders auffällig ist. Es wurde ferner untersucht, wie sich das Verhalten verändert, wenn statt nativer DNA denaturierte als Substrat angeboten und wenn die zweiwertigen Ionen durch EDTA komplexiert wurden. Eine Aktivitätsverminderung ist nicht auf das Auftreten von Inhibitoren zurückzuführen, sondern wahrscheinlich durch eine Konzentrationsverminderung an Enzym verursacht.

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Abbreviations

X. p.:

Xeroderma pigmentosum

Tris:

Tris (hydroxymethyl)-aminomethan

EDTA:

Äthylendiamintetraessigsäure

DNase:

Desoxyribonuclease

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Authors and Affiliations

  1. Physiologisch-Chemisches Institut der Johannes Gutenberg-Universität Mainz, Germany

    E. J. Zöllner, W. E. G. Müller & R. K. Zahn

  2. Universitäts-Hautklinik der Johannes Gutenberg-Universität Mainz, Germany

    G. Brehm & G. W. Korting

Authors
  1. E. J. Zöllner
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  2. W. E. G. Müller
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  3. R. K. Zahn
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  4. G. Brehm
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  5. G. W. Korting
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Zöllner, E.J., Müller, W.E.G., Zahn, R.K. et al. Möglichkeiten zur gezielten Diagnose durch Bestimmung der sauren Desoxyribonucleasen im Urin gesunder Kontrollpersonen und Xeroderma pigmentosum-Patienten. Klin Wochenschr 49, 1290–1294 (1971). https://doi.org/10.1007/BF01733085

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