Summary
Alcohol dehydrogenase (ADH) of different hexaploid wheat subspecies and varieties was investigated by isoelectric focusing in polyacrylamide gels. With this technique six ADH isoenzymes can be separated, while by the standard electrophoretic technique only three are visible. The ADH pattern revealed by isoelectric focusing is in full accordance with the hypothesis that the active ADH isozymes in hexaploid wheat are dimers composed of six possible combinations of subunits coded by triplicate structural genes.
Similar content being viewed by others
Literature
Bozzini, A.; Cubadda, T.; Quatrucci, E.: Esterases in Triticum and some related species. Proc. Fourth Int. Wheat Genet. Symp. 783–789 (1973)
Dale, G.; Latner, A.L.: Isoelectric Focusing in Polyacrylamide gels. Lancet 1, 847–848 (1968)
Fawcet, J.S.: Isoelectric Fractionation of Proteins on polyacrylamide gels. FEBS Letters 1, 81–88 (1968)
Hart, G.E.: Genetic Control of Alcohol Dehydrogenase Isozymes in Triticum dicoccum. Biochem. Genet 3, 617–625 (1969)
Hart, G.E.: Evidence for Triplicate Genes for Alcohol Dehydrogenase in Hexaploid Wheat. Proc. Nat. Acad. Sci. (USA) 66, 1136–1141 (1970)
Hart, G.E.: Alcohol Dehydrogenase Isozymes of Triticum: Dissociation and Recombination of Subunits. Mol. Gen. Genet. 111, 61–65 (1971)
Klose, J.; Spielmann, H.: Gel Isoelectric Focusing of Mouse Lactate Dehydrogenase: Heterogeneity of the Isoenzymes A4, and X4. Biochem. Genet. 13, 707–720 (1975)
Kohnert, K.D.; Schmidt, E.; Zühlke, H.; Fiedler, H.: Isoelectric Focusing of Insulins in Polyacrylamide Gel. J. Chromatog. 76, 263–267 (1973)
Leibenguth, F.: Activity tests of alcohol dehydrogenases in wheat, rye and Triticale. Experientia 33, 1434–1436 (1977)
Lutstorf, U.M.; Schürch, P.M.; von Wartburg, J.P.: Heterogeneity of Horse Liver Alcohol Dehydrogenase. Purification and Characterization of Multiple Molecular Forms. Eur. J. Biochem. 17, 497–508 (1970)
Malamud, D.; Drysdale, J.W.: Isoelectric Points of Proteins: A Table. Anal. Biochem. 86, 620–647 (1978)
Nakai, Y.; Tsunewaki, K.: Isozyme Variations in Aegilops and Triticum. I. Esterase Isozymes in Aegilops Studied using the gel Isoelectrofocusing Method. Jap. J. Genet. 46, 321–336 (1971)
Wrighley, C.: Gel electrofocusing — A technique for analyzing multiple protein samples by isoelectric focusing. Sci. Tools 15 17–23 (1968)
Wrighley, C: Analytical Fractination of Proteins According to Isoelectric Point by Gel Electrofocusing. Shandon Instr. Appl. 29 (1969)
Author information
Authors and Affiliations
Additional information
Communicated by L. Alföldi
Rights and permissions
About this article
Cite this article
Fejér, O., Orosz-Fejér, K. & Belea, A. Gel isoelectric focusing of wheat alcohol dehydrogenase. Theoret. Appl. Genetics 54, 37–39 (1979). https://doi.org/10.1007/BF00265706
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00265706