Skip to main content
SpringerLink
Log in

Genetic transformation in two potato cultivars with T-DNA from disarmed Agrobacterium

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Summary

Derivatives of potato (Solanum tuberosum cv.'s ‘Maris Bard’ and ‘Desiree’) transformed with disarmed T-DNA from genetically engineered Agrobacterium tumefaciens strains were isolated. The transformed plants were recovered from shoot-forming tumours induced by infection of wounds with mixedcultures of shoot-inducing A. tumefaciens strains T37 and either Agrobacterium strain LBA1834(pRAL1834), (Hille et al. 1983) or LBA4404(pBIN6; pRAL4404), (Bevan 1984). Two small-scale feasibility experiments gave at least four ‘Maris Bard’ plants transformed with pRAL1834 T-DNA and two ‘Desiree’ plants with pBIN6 T-DNA. The transformed ‘Maris Bard’ plants were morphologically abnormal and highly aneuploid. This was probably an unfortunate side-effect of a tissue culture-step introduced to promote the efficiency of shoot regeneration. The transformed ‘Desiree’ plants, in contrast, were isolated without promoting additional shoot-growth. They were morphologically normal, contained 47 and the euploid 48 chromosomes per cell respectively and had improved growth on media containing kanamycin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • An G (1985) High efficiency transformation of cultured tobacco cells. Plant Physiol 79:568–570

    Google Scholar 

  • An G, Watson BD, Chiang CC (1986) Transformation of tobacco, tomato, potato and Arabidopsis thaliana using a binary Ti vector system. Plant Physiol 81:301–305

    Google Scholar 

  • Bevan M (1984) Binary Agrobacterium vectors for plant transformation. Nucleic Acid Res 12:8711–8721

    Google Scholar 

  • Brisson N, Paszkowski J, Penswick JR, Gronenborn B, Potrykus I, Hohn T (1984) Expression of a bacterial gene in plants using a viral vector. Nature 310:511–514

    Google Scholar 

  • Burrell MM, Twell D, Karp A, Ooms G (1985) Expression of Ti T-DNA in differentiated tissues of potato (Solanum tuberosum cv. ‘Maris Bard’). Plant Mol Biol 5:213–222

    Google Scholar 

  • De Greve H, Leemans J, Hernalsteens JP, Thia-Toong L, De Beuckeleer M, Willmitzer L, Otten L, Van Montagu M, Schell J (1982) Regeneration of normal and fertile plants that express octopine synthase, from tobacco crown galls after deletion of tumour-controlling functions. Nature 300:752–755

    Google Scholar 

  • De Picker A, Herman L, Jacobs A, Schell J, Van Montagu M (1986) Frequencies of simultaneous transformation with different T-DNA and their relevance to the Agrobacterium and plant cell interaction. Mol Gen Genet 201:477–484

    Google Scholar 

  • Deshayes A, Herrera-Estrella L, Caboche M (1985) Liposomemediated transformation of tobacco mesophyll protoplasts by an Escherichia coli plasmid. EMBO J 4:2731–2739

    Google Scholar 

  • Fraley RT, Horsch RB, Matzke A, Chilton MD, Chilton WS, Sanders PR (1984) In vitro transformation of petunia cells by an improved method of cocultivation with Agrobacterium tumefaciens strains. Plant Mol Biol 3:371–378

    Google Scholar 

  • Gunn RE, Day PR (1986) In vitro culture in plant breeding In: Withers LA, Alderson PG (eds) Plant tissue culture and its agricultural applications. Butterworths, London, pp 313–336

    Google Scholar 

  • Hain R, Steinbiss HH, Schell J (1984) Fusion of Agrobacterium and E. coli spheroplasts with Nicotiana tabacum protoplasts: Direct gene transfer from micro-organism to higher plant. Plant Cell Rep 3:60–64

    Google Scholar 

  • Hasezawa S, Nagata T, Syono K (1981) Transformation of Vinca protoplasts mediated by Agrobacterium spheroplasts. Mol Gen Genet 182:206–210

    Google Scholar 

  • Horsch RB, Fry SE, Hoffmann NL, Eichholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 277:1229–1231

    Google Scholar 

  • Hille J, Wullems GJ, Schilperoort RA (1983) Non-oncogenic T-region mutants of Agrobacterium tumefaciens do transfer T-DNA into plant cells. Plant Mol Biol 2:155–163

    Google Scholar 

  • Karp A, Nelson RS, Thomas E, Bright SWJ (1982) Chromosome variation in protoplast-derived potato plants. Theor Appl Genet 63:265–272

    Google Scholar 

  • Karp A, Bright SWJ (1985) On the causes and origins of somaclonal variation. In: Miflin BJ (ed) Oxford surveys of plant molecular and cell biology. Oxford University Press, London, pp 199–325

    Google Scholar 

  • Krens FA, Molendijk L, Wullems GJ, Schilperoort RA (1982) In vitro transformation of plant protoplasts with Ti plasmid DNA. Nature 296:72–74

    Google Scholar 

  • Kumar D, Wareing PF (1974) Studies on tuberization of Solanum andigena II growth hormones and tuberization. New Phytol 73:833–840

    Google Scholar 

  • Larkin PJ, Scowcroft WR (1981) Somaclonal variation — a novel source of variability from cell culture for plant improvement. Theor Appl Genet 60:197–214

    Google Scholar 

  • Lovell PH, Booth A (1967) Effects of gibberellic acid on growth, tuber formation and carbohydrate distribution in Solanum tuberosum. New Phytol 66:525–537

    Google Scholar 

  • Marton L, Wullems GJ, Molendijk L, Schilperoort RA (1979) In vitro transformation of cultured cells from Nicotiana tabacum by Agrobacterium tumefaciens. Nature 277:129–131

    Google Scholar 

  • Muller A, Manzara T, Lurquin PF (1984) Crown gall transformation of tobacco callus by co-cultivation with Agrobacterium tumefaciens. Biochem Biophys Res Commun 123:458–462

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497

    CAS  Google Scholar 

  • Ohgaware T, Uchimiya H, Harada H (1983) Uptake of liposome-encapsulating plasmid DNA by plant protoplasts and molecular fate of foreign DNA. Protoplasma 116:145–148

    Google Scholar 

  • Okado K, Hasazawa S, Syono K, Nagata T (1985) Further evidence for the transformation of vinca rosea protoplasts by Agrobacterium tumefaciens spheroplasts. Plant Cell Rep 4:133–136

    Google Scholar 

  • Ooms G, Hooykaas PJJ, Moolenaar G, Schilperoort RA (1981) Crown gall plant tumors of abnormal morphology, induced by Agrobacterium tumefaciens carrying mutated octopine Ti plasmids: analysis of T-DNA functions. Gene 14:33–50

    Google Scholar 

  • Ooms G, Hooykaas PJJ, Van Veen RJM, Van Beelen P, Regensburg TJG, Schilperoort RA (1982 a) Octopine Ti-plasmid deletion mutants of Agrobacterium tumefaciens with emphasis on the right side of the T-region. Plasmid 7:15–29

    Google Scholar 

  • Ooms G. Bakker A, Molendijk L, Wullems GJ, Gordon MP, Nester EW, Schilperoort RA (1982b) T-DNA organization in homogeneous and heterogeneous octopine type crown gall tissues of Nicotiana tabacum. Cell 30:589–597

    Google Scholar 

  • Ooms G, Molendijk L, Schilperoort RA (1982c) Double infection of tobacco plants by two complementing octopine T-region mutants of Agrobacterium tumefaciens. Plant Mol Biol 1:217–227

    Google Scholar 

  • Ooms G, Karp A, Roberts J (1983) From tumour to tuber: tumour cell characteristics and chromosome numbers of crown gall-derived tetraploid potato plants (Solanum tuberosum cv. ‘Maris Bard’). Theor Appl Genet 66:169–172

    Google Scholar 

  • Ooms G, Karp A, Burrell MM, Twell D, Roberts J (1985) Genetic modification of potato development using Ri T-DNA. Theor Appl Genet 70:440–446

    Google Scholar 

  • Otten LABM, Schilperoort RA (1978) A rapid microscale method for the detection of lysopine and nopaline dehydrogenase activities. Biochim Biophys Acta 527:494–500

    Google Scholar 

  • Pollock K, Barfield DG, Robinson SJ, Shields R (1985) Transformation of protoplast-derived cell colonies and suspension cultures by Agrobacterium-tumefaciens. Plant Cell Rep 4:202–205

    Google Scholar 

  • Potrykus I, Shillito RD, Saul MW, Paszkowski J (1985) Direct gene transfer: state of the art and future potential. Plant Mol Rep 3:117–128

    Google Scholar 

  • Sacristan MD, Melchers G (1977) Regeneration of plants from habituated and Agrobacterium-transformed single clones of tobacco. Mol Gen Genet 152:111–117

    Google Scholar 

  • Shepard JF, Bidney D, Shahin E (1980) Potato protoplasts in crop improvment. Science 208:17–24

    Google Scholar 

  • Slater JW (1963) Mechanisms of tuber initiation. In: Ivins JD, Milthorpe FL (eds) The growth of the potato. Butterworth, London, pp 114–120

    Google Scholar 

  • Smith EF, Townsend CO (1907) A plant-tumor of bacterial origin. Science 25:671–673

    Google Scholar 

  • Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517

    CAS  PubMed  Google Scholar 

  • Sree Ramulu K, Dijkhuis P, Roest S (1983) Phenotypic variation and ploidy level of plants regenerated from protoplasts of tetraploid potato (Solanum tuberosum L cv. ‘Bintje’). Theor Appl Genet 65:329–338

    Google Scholar 

  • Tizio R (1972) Effet de la lumière sur la tubérisation de la pomme de terre. Potato Res 15:257–262

    Google Scholar 

  • Van Slogteren GMS, Hoge JHC, Hooykaas PJJ, Schilperoort RA (1983) Clonal analysis of heterogeneous crown gall tissues induced by wild-type and shooter mutant strains of Agrobacterium tumefaciens expression of T-DNA genes. Plant Mol Biol 2:321–335

    Google Scholar 

  • Waldron C, Murphy EB, Roberts JL, Gustafson GD, Armour SL, Malcolm SK (1985) Resistance to hygromycin B. Plant Mol Biol 5:103–108

    Google Scholar 

Download references

Author information

Author notes

  1. J. Hille

    Present address: Department of Molecular Biology, Agricultural University of Wageningen, De Dreyen 11, NL-6703, BC Wageningen, The Netherlands

Authors and Affiliations

  1. Department of Biochemistry, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts, UK

    G. Ooms, M. M. Burrell & A. Karp

  2. Department of Molecular Genetics, Plant Breeding Institute, Maris Lane, CB2 2LQ, Trumpington, Cambridge, UK

    M. Bevan

  3. Department of Plant Molecular Biology, University of Leiden, Wassenaarseweg 66, NL-2333, AL Leiden, The Netherlands

    J. Hille

Authors
  1. G. Ooms
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. M. Burrell
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Karp
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Bevan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. Hille
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by R. Riley

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ooms, G., Burrell, M.M., Karp, A. et al. Genetic transformation in two potato cultivars with T-DNA from disarmed Agrobacterium . Theoret. Appl. Genetics 73, 744–750 (1987). https://doi.org/10.1007/BF00260785

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00260785

Key words

Search

Navigation