Summary
The structure of mitochondrial DNA (mt-DNA) from sugarbeet (Beta vulgaris L.) has been studied by biochemical methods and electron microscopy. It was found to be complex multipartite consisting of two main classes of molecules: high molecules weight (HMW) mtDNA and low molecular weight (LMW) mtDNA. The HMW mtDNA consists of rosette-like structures and globules resembling chromomeres (150–200nm). A typical rosette has a protein core and radially stemming closed DNA loops (from 0.6-1.5 μm). The number of loops in a rosette varies from 16–30. The bulk of HMW mtDNAs are represented by interconnected rosettes (total contour length about 130–160 μm, 403–496 kbp). Such large circular DNAs may be evidence of the master chromosome arrangement of the sugarbeet genome. Globules and rosettes are interconnected by thick and thin DNA fibrils, along which nucleosome- and nucleomere-like structures are distributed. The LWM mtDNA is composed of two groups of supercoiled circular molecules, 0,2–1.5 μm and 0.02–0.05 μm in size. Electrophoretic analysis demonstrated that LWM mtDNA is represented by minicircle plasmid-like DNA molecules of 1.3, 1.4 and 1.6 kbp.
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References
Aldrich J, Cattolica RA (1981) Isolation and characterization of chloroplast DNA from the marine chromophyte, Olisthodiscus: electron microscopic visualization of isomeric molecular forms. Plant Physiol 68:641–647
Caron F, Jacq C, Rouviere-Yaniv J (1979) Characterization of a histone-like protein extracted from yeast mitochondria. Proc Natl Acad Sci USA 76:4265–4269
Chen CW, Thomas CA Jr (1980) Recovery of DNA segments from agarose gels. Anal Biochem 101:339–341
Georgiev GP, Bakaev VV (1978) Three levels of the structural organization of chromosomes in eukaryotes. Mol Biol (USSR) 12:1205–1230
Glazkov MV (1986) Rat liver cell nucleoids: electron microscopy analysis. Mol Biol (USSR) 20:1692–1703
Goblet JP, Boutry M, Duc G, Briquet M (1983) Mitochondrial plasmid-like molecules in fertile and male sterile Vicia faba L. Plant Mol Biol 2:305–309
Gornung EM, Polyakov VYu, Chentsov YuS (1986) Levels of DNA compactization in interphase and mitotic chromosomes of higher plants. Tsitologiya 28:911–914
Handa H, Tsunewaki K, Kunisada T, Yamagishi H (1984) Small circular DNA molecules in wheat mitochondria. Mol Gen Genet 194:368–372
Jamet-Vierney C, Begel O, Belcour L (1980) Senescence in Podospora auserina: amplification of a mitochondrial DNA sequence. Cell 21:189–194
Kiseleva EV, Lihoshvai EV, Serdyukova NA, Khristolyubova NB (1986) An electron microscopic analysis of the levels of the structural organization of Escherichia coli chromosome. Dokl Akad Nauk USSR 289:1235–1237
Kleinschmidt AK (1968) Monolayer techniques in electron microscopy of nucleic acid molecules. In: Grossman L, Moldave K (eds) Methods in enzymology, vol 12B. Academic Press, New York London, pp 361–377
Kuroiwa T (1982) Mitochondrial nuclei. Int Rev Cytol 75:1–58
Leaver ChJ, Forde BG, Dixon LK, Fox TD (1982) Mitochondrial genes and cytoplasmically inherited variation in higher plants. In: Slonimski P, Borst P, Attardi G (eds) Mitochondrial genes. Cold Spring Harbor Laboratory Press, Cold Spring Harbor/NY, pp 457–470
Leaver CJ, Gray MW (1982) Mitochondrial genome organization and expression in higher plants. Ann Rev Plant Physiol 33:373–402
Levings CS III (1983) The plant mitochondrial genome and its mutants. Cell 32:659–661
Levings CS III, Pring DR (1978) The mitochondrial genome of higher plants. Stadler Genet Symp 10:77–94
Lonsdale DM (1984) A review of the structure and organization of the mitochondrial genome of higher plants. Plant Mol Biol 3:201–206
Maniatis T, Fritsch EF, Saulbrook J (1982) A laboratory manual molecular cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor/NY
Mikulska E, Odintsova MS, Turischeva MS (1970) Electron microscopy of DNA in mitochondria of pea seedlings. J Ultrastruct Res 32:258–267
Mulligan RM, Walbot V (1986) Gene expression and recombination in plant mitochondrial genomes. Trend Genet 2:263–266
Negruk VI, Eisner GI, Redichkina TD, Dumanskaya NN, Cherny DI, Alexandrov AA, Shemyakin MF, Butenko RG (1986) Diversity of Vicia faba circular mtDNA in whole plants and suspension cultures. Theor Appl Genet 72:541–547
Powling A (1982) Restriction endonuclease analysis of mitochondrial DNA from sugarbeet with normal and malesterile cytoplasms. Heredity 49:117–120
Powling A, Ellis TWN (1983) Studies on the organelle genomes of sugarbeet with male-fertile and male-sterile cytoplasms. Theor Appl Genet 65:323–328
Quetier F, Vedel F (1977) Heterogeneous population of mitochondrial DNA molecules in higher plants. Nature 268:365–368
Renger HC, Welstenholme DR (1972) The form and structure of kinetoplast DNA of crithidia. J Cell Biol 54:346–364
Sederoff RR (1984) Structural variation in mitochondrial DNA. Adv Genet 22:1–108
Suzuki T, Kawano S, Kuroiwa T (1982) Structure of three-dimensionally rod-shaped mitochondrial nucleoids isolated from the slime mould Physarum polycephalum. J Cell Sci 58:241–261
Synenki RM, Levings CS, Shan DM (1978) Physicochemical characterization of mitochondrial DNA from soybean. Plant Physiol 61:460–464
Thomas CM (1986) The nucleotide sequence and transcription of minicircular mitochondrial DNA's associated with male-fertile and cytoplasmic male-sterile lines of sugarbeet. Nucleic Acids Res 14:9353–9370
van Bruggen EFJ, Runner CM, Borst P, Ruttenberg CJCM, Kroon AM, Schuurmans-Steknoven FMAH (1968) Mitochondrial DNA. II. Electron microscopy of DNA released from mitochondria by osmotic shock. Biochim Biophys Acta 161:402–414
van Tiule JC, Pherson ML (1979) A compact form of rat liver mitochondrial DNA stabilized by bound proteins. J Biol Chem 254:6044–6055
Wiegand RC, Godson GN, Radding CM (1975) Specificity of S1 nuclease from Aspergillus oryzae. J Biol Chem 250:8848–8855
Zatsepina OV, Polyakov VYu, Chentsov YuS (1983) An electron microscopic study of chromonema and chromomeres in mitotic and interphase chromosomes. Tsitologiya 25:123–129
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Dudareva, N.A., Kiseleva, E.V., Boyarintseva, A.E. et al. Structure of the mitochondrial genome of Beta vulgaris L.. Theoret. Appl. Genetics 76, 753–759 (1988). https://doi.org/10.1007/BF00303522
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DOI: https://doi.org/10.1007/BF00303522