Summary
The polymerase chain reaction (PCR) is particularly well suited for the detection of rare sequences. Taking advantage of the recent isolation of sequences associated with stigma self-incompatibility inBrassica oleracea, we used PCR amplifications with primers synthesized to the S6 cDNA sequence, to demonstrate the presence of mRNA homologous to stigmaS-locus gene (SLG) in anthers during early microsporogenesis. In addition, otherS-locus-related (SLR) sequences were shown to be transcribed in sexual as well as in vegetative tissues (roots, leaves), suggesting that the SLG family might be involved not only in pollen-stigma recognition, but more generally in various forms of plant cell signalling processes. This information corroborates the recent discovery of a cDNA-deduced protein kinase from maize roots, whose extracellular receptor displays high homology withBrassica S-locus-specific glycoproteins.
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Communicated by H.F. Linskens
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Guilluy, C.M., Trick, M., Heizmann, P. et al. PCR detection of transcripts homologous to the self-incompatibility gene in anthers ofBrassica . Theoret. Appl. Genetics 82, 466–472 (1991). https://doi.org/10.1007/BF00588600
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DOI: https://doi.org/10.1007/BF00588600