Skip to main content
Log in

Organelle DNA polymorphism in apple cultivars and rootstocks

  • Originals
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Summary

Restriction fragment length polymorphisms (RFLPs) have been used to detect chloroplast (cp) and mitochondrial (mt) DNA variation among 18 apple cultivars and three rootstocks. The distribution of RFLP patterns allowed the assignment of these genotypes into three groups of cytoplasmic relatedness. Our results also demonstrate maternal inheritance of cp- and mtDNAs in apple. Thus, the organelle DNA assay provides a convenient and reliable method to assess cytoplasmic diversity within the apple germ-plasm collection and to trace the maternal lineages involved in the evolution of apple.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13

    CAS  PubMed  Google Scholar 

  • Fragoso LL, Nichols SE, Levings CS III (1989) Rearrangements in maize mitochondrial genes. Genome 31:160–168

    Google Scholar 

  • Gracen PE, Grogan CO (1974) Diversity and suitability for hybrid production of different sources of cytoplasmic male sterility in maize. Agron J 66:654–657

    Google Scholar 

  • Hanson MR, Conde MF (1985) Functioning and variation of cytoplasmic genomes: Lessons from cytoplasmic-nuclear interactions affecting male fertility in plants. Int Rev Cytol 94:213–267

    Google Scholar 

  • Huh TY, Gray MW (1982) Conservation of ribosomal RNA gene arrangement in the mitochondrial DNA of angiosperms. Plant Mol Biol 1:245–249

    Google Scholar 

  • Leaver CJ, Gray MW (1982) Mitochondrial genome organization and expression in higher plants. Annu Rev Plant Physiol 33:373–402

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.

    Google Scholar 

  • Marechal L, Runeberg-Roos P, Grienenberger JM, Colin J, Weil JH, Lejeune B, Quetier F, Lonsdale DM (1987) Homology in the region containing a tRNATrp gene and a (complete or partial) tRNAPro gene in wheat mitochondrial and chloroplast genomes. Curr Genet 12:91–98

    Google Scholar 

  • Mikami T, Sugiura M, Kinoshita T (1984) Molecular heterogeneity in mitochondrial and chloroplast DNAs from normal and male sterile cytoplasms in sugar beets. Curr Genet 8:319–322

    Google Scholar 

  • Morikami A, Nakamura K (1987a) Structure and expression of pea mitochondrial F1 ATPase α-subunit gene and its pseudogene involved in homologous recombination. J Biochem 101:967–976

    Google Scholar 

  • Morikami A, Nakamura K (1987b) The pea mitochondrial AT-Pase subunit 9 gene is located upstream of the ATPase α-subunit gene. Nucleic Acids Res 15:4692

    Google Scholar 

  • Palmer JD (1987) Chloroplast DNA evolution and biosystematic uses of chloroplast DNA variation. Am Nat 130:S6-S29

    Article  CAS  Google Scholar 

  • Pring DR, Lonsdale DM (1985) Molecular biology of higher plant mitochondrial DNA. Int Rev Cytol 97:1–46

    Google Scholar 

  • Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc Natl Acad Sci USA 81:8014–8018

    PubMed  Google Scholar 

  • Schuster W, Brennicke A (1988) Interorganellar sequence transfer: Plant mitochondrial DNA is nuclear, is plastid, is mitochondrial. Plant Sci 54:1–10

    Google Scholar 

  • Sears BB (1980) Elimination of plastids during spermatogenesis and fertilization in the plant kingdom. Plasmid 4:233–255

    Google Scholar 

  • Senda M, Harada T, Mikami T, Sugiura M, Kinoshita T (1991) Genomic organization and sequence analysis of the cytochrome oxidase subunit II gene from normal and male-sterile mitochondria in sugar beet. Curr Genet 19:175–181

    CAS  PubMed  Google Scholar 

  • Stern DB, Lonsdale DM (1982) Mitochondrial and chloroplast genomes of maize have a 12 kb DNA sequence in common. Nature 299:698–702

    CAS  PubMed  Google Scholar 

  • Sugiura M, Kusuda J (1979) Molecular cloning of tobacco chloroplast ribosomal RNA genes. Mol Gen Genet 172:137–141

    Google Scholar 

  • Tsunewaki K (1989) Plasmon diversity in Triticum and Aegilops and its implication in wheat evolution. Genome 31:143–154

    Google Scholar 

  • Vinterhalter DV, James DJ (1986) The use of peroxidase polymorphism in the identification of Malling and Malling Merton apple rootstocks. J Hortic Sci 61:147–152

    Google Scholar 

  • Whatley JM (1982) Ultrastructure of plastid inheritance: green algae to angiosperms. Biol Rev 57:527–569

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by K. Tsunewaki

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ishikawa, S., Kato, S., Imakawa, S. et al. Organelle DNA polymorphism in apple cultivars and rootstocks. Theoret. Appl. Genetics 83, 963–967 (1992). https://doi.org/10.1007/BF00232957

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00232957

Key words

Navigation