Summary
Bruchids (genus Callosobruchus) are among the most destructive insect pests of mungbeans and other members of the genus, Vigna. Genetic resistance to bruchids was previously identified in a wild mungbean relative, TC1966. To analyze the underlying genetics, accelerate breeding, and provide a basis for map-based cloning of this gene, we have mapped the TC1966 bruchid resistance gene using restriction fragment length polymorphism (RFLP) markers. Fifty-eight F2 progeny from a cross between TC1966 and a susceptible mungbean cultivar were analyzed with 153 RFLP markers. Resistance mapped to a single locus on linkage group VIII, approximately 3.6 centimorgans from the nearest RFLP marker. Because the genome of mungbean is relatively small (estimated to be between 470 and 560 million base pairs), this RFLP marker may be suitable as a starting point for chromosome walking. Based on RFLP analysis, an individual was also identified in the F2 population that retained the bruchid resistance gene within a tightly linked double crossover. This individual will be valuable in developing resistant mungbean lines free of linkage drag.
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References
AVRDC (1988) Mungbean: Proceedings 2nd Symposium. Asian Vegetable Research and Development Center, Shanhua, Taiwan
Arumuganathan K, Earle E (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9:208–218
Beavis WD, Grant D (1991) A linkage map based on information from four F2 populations of maize (Zea mays L.). Theor Appl Genet 82:636–644
Bennett MD, Smith JP (1976) Nuclear DNA amounts in angiosperms. Phil Trans R Soc Lond Ser B 274:227–273
Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21
Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction fragments to high specific activity. Anal Biochem 132:6–13
Fujii K, Ishimoto M, Kitamura K (1989) Patterns of resistance to bean weevils (Bruchidae) in Vigna radiata — sublobata complex inform the breeding of new resistant varieties. Appl Entomol Zool 24:126–132
Gallun RL, Patterson FL (1977) Monosomic analysis of wheat for resistance to hessian-fly. J Hered 68:223–226
Ganal MW, Young ND, Tanksley SD (1989) Pulsed-field gel electrophoresis and physical mapping of large DNA fragments in the Tm-2a region of chromosome 9 in tomato. Mol Gen Genet 215:395–400
Huen M, Kennedy AE, Anderson JA, Lapitan NLV, Sorrells ME, Tanksley SD (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vultgare). Genome 34:437–447
Joseph LS, Bouwkamp JC (1978) Karyomorphology of several species of Phaseolus and Vigna. Cytologia 43:595–600
Keim P, Diers BW, Olson TC, Shoemaker RC (1990) RFLP mapping in soybean: association between marker loci and variation in quantitative traits. Genetics 126:735–742
Lakshminarayana A, Khush GS (1977) New genes for resistance to the brown planthopper in rice. Crop Sci 17:96–100
Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLPs linkage maps. Genetics 121:185–199
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER; an interactive computer program for constructing genetic linkage maps of experimental and natural populations. Genomics 1:174
Landry BS, Kesseli RV, Farrara B, Michelmore RW (1987) A genetic map of lettuce (Lactuca sativa L.) with restriction fragment length polymorphism, isozyme, disease resistance and morphological markers. Genetics 116:331–337
Martin GB, Williams JGK, Tanksley SD (1991) Rapid identification of markers linked to a Pseudomonas resistance gene in tomato using random primers and near-isogenic lines. Proc Natl Acad Sci USA 88:2336–2340
Menancio-Hautea D, Kumar L, Danesh D, Young N (1992) A genome map for mungbean [Vigna radiata (L.) Wilczek] based on DNA genetic markers. In: Stephen O'Brien (ed) Genetic Maps — 1992 (6th edition). Cold Spring Harbor Laboratory Press (in press)
Messeguer R, Ganal M, de Vicente MC, Young ND, Bolkan H, Tanksley SD (1991) High resolution RFLP map around the root knot nematode resistance gene (Mi) in tomato. Theor Appl Genet 82:529–536
Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregation analysis: a rapid method to detect markers in specific genome regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832
Murray MG, Palmer JD, Cuellar RE, Thompson WF (1979) Deoxyribonucleic acid sequence organization in the mungbean genome. Biochemistry 18:5259–5264
Nienhuis J, Helentjaris T, Slocum M, Ruggero B, Schaefer A (1987) Restriction fragment length polymorphism analysis of loci associated with insect resistance in tomato. Crop Sci 27:797–803
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning (2nd edn.). Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Sharp PJ, Chao S, Desai S, Gale MD (1989) The isolation, characterization and application in the Triticeae of a set of wheat RFLP probes identifying each homeologous chromosome arm. Theor Appl Genet 78:342–348
Singh SR, Allen DJ (1979) Cowpea pests and diseases. International Institute of Tropical Agriculture, Ibadan, Nigeria
Southern E (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Steinmetz M, Minard K, Harvath S, McNicholas J, Srenlinger J, Wake C, Long E, Mach B, Hood L (1981) A molecular map of the immune response region of the major histocompatibility complex of the mouse. Nature 300:35–42
Talekar NS (1988) Biology, damage and control of bruchid pests of mungbean. In: Mungbean: Proceedings of the Second International Symposium, Asian Vegetable Research and Development Center, Shanhua, Taiwan, pp 329–342
Tanksley SD, Miller JC, Paterson AH, Bernatzky R (1988) Molecular mapping of plant chromosomes. In: Gustafson JF, Appels R (eds) Chromosome structure and function. Plenum Press, New York, pp 157–173
Young ND (1990) Potential applications of map-based cloning to plant pathology. Physiol Mol Pl Path 37:81–94
Young ND, Tanksley SD (1989a Restriction fragment length polymorphism maps and the concept of graphical genotypes. Theor Appl Genet 77:95–101
Young ND, Tanksley SD (1989b) RFLP analysis of the size of chromosomal segments retained around the Tm-2 locus of tomato during backcross breeding. Theor Appl Genet 77:353–359
Young ND, Zamir D, Ganal MW, Tanksley SD (1988) Use of isogenic lines and simultaneous probing to identify DNA markers tightly linked to the Tm-2 a gene in tomato. Genetics 120:579–585
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Communicated by F. Salamini
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Young, N.D., Kumar, L., Menancio-Hautea, D. et al. RFLP mapping of a major bruchid resistance gene in mungbean (Vigna radiata, L. Wilczek). Theoret. Appl. Genetics 84, 839–844 (1992). https://doi.org/10.1007/BF00227394
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DOI: https://doi.org/10.1007/BF00227394