Skip to main content
SpringerLink
Log in

Somatic fusion for combining virus resistances in Solanum tuberosum L.

  • Originals
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Summary

Dihaploid genotypes of potato, containing the dominant allele for extreme resistance to PVX and/or PVY, were used in a fusion program in order to analyze the behaviour of the two monogenetic resistances after fusion. Eighteen different fusion combinations were performed and regenerated hybrids were tested by ELISA for their virus resistance. In most of the combinations an addition of the two qualities was found, but a few deviating clones were observed. The possible reasons for the phenotypic disappearance of resistant alleles are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Austin S, Baer MA, Helgeson JP (1985) Transfer of resistance to potato leaf roll virus from Solarium brevidens into Solanum tuberosum by somatic fusion. Plant Sci 39:75–82

    Google Scholar 

  • Binding H, Nehls R, Schieder O, Sopory SK, Wenzel G (1978) Regeneration of mesophyll protoplasts isolated from dihaploid clones of Solanum tuberosum. Physiol Plant 43:52–54

    Google Scholar 

  • Chaput MH, Sihchakr D, Ducreux G, Marie D, Barghi N (1990) Somatic hybrid plants produced by electrofusion between dihaploid potatoes: BF 15 (H1), Aminca (H6), Cardinal (H3). Plant Cell Rep 9:411–414

    Google Scholar 

  • Deimling S, Zitzlsperger J, Wenzel G (1988) Somatic fusion for breeding tetraploid potatoes. Plant Breed 101:181–189

    Google Scholar 

  • Gleba YY, Sytnik KM (1984) Protoplast fusion, genetic engineering in higher plants. Springer, Berlin

    Google Scholar 

  • Glimelius K, Fahlesson J, Landgren M, Sjödin C, Sundberg E (1991) Gene transfer via somatic hybridization in plants. Trends Biotechnol 9:24–301

    Google Scholar 

  • Haberlach GT, Cohen BA, Reichert NA, Baer MA, Towill LE, Helgeson JP (1985) Isolation, culture and regeneration of protoplasts from potato and several related Solanum species. Plant Sci 39:67–74

    Google Scholar 

  • Mattheij WM, Puite KJ (1992) Tetraploid potato hybrids through protoplast fusions and analysis on their performance in the field. Theor Appl Genet 83:807–812

    Google Scholar 

  • Möllers C, Wenzel G (1992) Somatic hybridization of diploid potato protoplasts as a tool for potato breeding. Bot Acta 105:133–139

    Google Scholar 

  • Möllers C, Hofferbert R, Schriener A, Wenzel G (1992) Ergebnisse eines Feldanbaus tetraploider somalischer Kartoffelhybriden. Arbeitst Saatzuchtl Gumpenstein (in press)

  • Pehu E, Gibson RW, Jones MGK, Karp A (1990) Studies on the genetic basis of resistance to potato leaf roll virus, potato virus Y and potato virus X in Solanum brevidens using somatic hybrids of Solanum brevidens and Solanum tuberosum. Plant Sci 69:95–101

    Google Scholar 

  • Saghai-Maroof MA, Soliman KM, Jörgensen RA, Allard RW (1984) Ribosomal spacer length polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc Natl Acad Sci USA 81:15–20

    Google Scholar 

  • Schilde-Rentschler L, Ninnemann H (1988) Kombination von Kartoffellinien durch Protoplastenfusion zur Regeneration züchterisch nutzbarer Hybridpflanzen. Vortr Pflanzenzücht 14:149–160

    Google Scholar 

  • Shaw CR, Prasad R (1970) Starch gel electrophoresis of enzymes — a compilation of recipes. Biochem Genet 4:297–320

    CAS  PubMed  Google Scholar 

  • Vallejos CE (1983) Enzyme activity staining. In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding; part B. Elsevier, Amsterdam, pp 469–516

    Google Scholar 

  • Waara S, Tegelstorm H, Wallin A, Eriksson T (1989) Somatic hybridization between anther-derived dihaploid clones of potato (Solanum tuberosum L.) and the identification of hybrid plants by isozyme analysis. Theor Appl Genet 77:49–56

    Google Scholar 

  • Waara S, Pijnacker L, Ferwerda MA, Wallin A, Eriksson T (1992) A cytogenetic and phenotypic characterization of somatic hybrid plants obtained after fusion of two different dihaploid clones of potato (Solanum tuberosum L.). Theor Appl Genet 85:470–479

    Google Scholar 

  • Wenzel G, Schieder O, Przewozny T, Sopory SK, Melchers G (1979) Comparison of single-cell-culture-derived Solanum tuberosum L. plants and a model of their application in breeding programs. Theor Appl Genet 59:49–55

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Federal Biological Research Center for Agriculture and Foresty, Institute for Resistance Genetics, D-8059, Grünbach, Germany

    N. Q. Thach, U. Frei & G. Wenzel

Authors
  1. N. Q. Thach
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. U. Frei
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. Wenzel
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by I. Potrykus

On leave from the Agricultural University No.1, Hanoi, Vietnam

Rights and permissions

Reprints and permissions

About this article

Cite this article

Thach, N.Q., Frei, U. & Wenzel, G. Somatic fusion for combining virus resistances in Solanum tuberosum L.. Theoret. Appl. Genetics 85, 863–867 (1993). https://doi.org/10.1007/BF00225030

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00225030

Key words

Search

Navigation