Abstract
As a result of earlier breeding efforts, portions of the genome of “Basmati 370” have been introgressed into a rice breeding line, B8462T3-710. Cooked-kernel elongation was increased in this breeding line to a level equal to that of “Basmati 370”. The objective of this study was to identify and locate quantitative trait loci (QTLs) associated with cooked-kernel elongation in an F3 population derived from a cross between B8462T3710 and the reduced-elongation recurrent parent variety, Dellmont. DNA from the parental lines and “Basmati 370” as a control, were screened for RFLPs using 170 clones chosen to cover the rice genome at intervals of 8 cM on average. Eighteen markers identified RFLPs common to Basmati 370 and B8462T3-710, but different from Dellmont, suggesting possible associations with kernel elongation. The B8462T3-710/Dellmont F3 population was analyzed for segregation of those RFLPs and for kernel elongation. Analysis of variance of the kernel elongation ratio revealed that two markers, 14.6 cM apart on chromosome 8, are significantly associated with this trait (RZ323 P ≤0.005, RZ562 P ≤0.05). Interval mapping suggests a single QTL with a close proximity to RZ323. This QTL was tested in F6 lines derived from the same cross and the presence of the B8462T3-710 segment detected by RZ323 caused a highly significant increase of the kernel elongation ratio (P ≤0.04). In addition, the QTL for kernel elongation and a gene for aroma, which are major components of the grain quality characteristics of Basmati-type rices, showed linkage. The availability of linked markers to the QTL may facilitate early selection for kernel elongation in rice breeding programs.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ahn SN, Bollich CN, Tanksley SD (1992) RFLP tagging of a gene for aroma in rice. Theor Appl Genet 84:825–828
Beavis WD, Grant D, Albertsen M, Fincher R (1991) Quantitative trait loci for plant height in four maize populations and their associations with qualitative genetic loci. Theor Appl Genet 83:141–145
Diers BW, Keim P, Fehr WR, Shoemaker RC (1992) RFLP analysis of soybean seed protein and oil content. Theor Appl Genet 83:608–612
Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction fragments to a high specific activity. Anal Biochem 132:6–13
Glaszmann JC (1987) Isozymes and classification of Asian rice varieties. Theor Appl Genet 74:21–30
Juliano BO (1979) The chemical basis of rice grain quality. Proc Wkshp on chemical aspects of rice grain quality. Int Rice Res Inst, Los Banos, Laguna, Philippines, pp 69–90
Juliano BO, Perez CM (1984) Results of a collaborative test on the measurement of grain elongation of milled rice during cooking. J Cereal Sci 2:281–292
Keim P, Diers BW, Olson TC, Shoemaker RC (1990) RFLP mapping in soybean: association between marker loci and variation in quantitative traits. Genetics 126:735–742
Khush GS, Paule CM, De La Cruz NM (1979) Rice grain quality evaluation and improvement at IRRI. Proc Wkshp on chemical aspects of rice grain quality. Int Rice Res Inst, Los Banos, Laguna, Philippines, pp 21–31
Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175
Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: An interactive computer package for maps of experimental and natural populations. Genomics 1:174–181
McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829
Paterson AH, Lander ES, Hewitt ID, Peterson S, Lincoln SE, Tanksley SD (1988) Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms. Nature 335:721–726
Paterson AH, Damon S, Hewitt JD, Zamir D, Rabinowitch HD, Lincoln SE, Lander ES, Tanksley SD (1991) Mendelian factors underlying quantitative traits in tomato: comparison across species, generations, and environments. Genetics 127:181–197
Reddy PR, Sathyanarayanaiah K (1980) Inheritance of aroma in rice. Indian J Genet Plant Breed 40:327–329
Sood BC, Siddiq EA, Zaman FU (1979) The mechanism of kernel elongation in rice. Indian J Genet Plant Breed 39:457–460
Stuber CW, Edwards MD, Wendel JF (1987) Molecular markerfacilitated investigations of quantitative trait loci in maize. II. Factors influencing yield and its component traits. Crop Sci 27:639–648
Tanksley SD, Hewitt J (1988) Use of molecular markers in breeding for soluble solids content in tomato — a re-examination. Theor Appl Genet 75:811–823
Wang ZY, Tanksley SD (1989) Restriction fragment length polymorphism in Oryza sativa L. Genome 32:1113–1118
Author information
Authors and Affiliations
Additional information
Communicated by F. Salamini
Rights and permissions
About this article
Cite this article
Ahn, S.N., Bollich, C.N., McClung, A.M. et al. RFLP analysis of genomic regions associated with cooked-kernel elongation in rice. Theoret. Appl. Genetics 87, 27–32 (1993). https://doi.org/10.1007/BF00223739
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00223739