Abstract
A physical map including 40 translocation breakpoints has been constructed in rye by means of synaptonemal complex (SC) analysis of well-paired pachytene quadrivalents. The chromosome arms involved in such translocations were previously identified either from mitotic C-banding analysis or from the meiotic configurations observed in the progenies of crosses with a rye line having multiple chromosome rearrangements. The synaptonemal complexes formed by some translocation homozygotes were also analyzed, the relative pachytene SC length of their translocated chromosomes being compared to that observed in the corresponding translocation heterozygotes. In the translocations in which the position of the breakpoint could be well defined from mitotic C-banding analysis, a good correspondence between the relative position of the point showing partner exchange in the pachytene quadrivalents and the actual location of the breakpoint was established. It is concluded that the mapping of translocation breakpoints by SC analysis of pachytene quadrivalents provides a more accurate estimate of the position of the breakpoints than that obtained from mitotic C-banding analysis, due to the lack of evenly-distributed interstitial C-bands in most rye chromosomes. The distribution of the breakpoints along the chromosomes in relation to their spontaneous or induced origin is also discussed.
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Abirached-Darmency M, Zickler D, Cauderon Y (1983) Synaptonemal complex and recombination nodules in rye (Secale cereale) Chromosoma 88:299–306
Alonso-Blanco C, Goicoechea PG, Roca A, Giraldez R, (1993) A cytogenetic map on the entire length of rye chromosome 1R, including one translocation breakpoint, three isozyme loci and four C-bands. Theor Appl Genet 85:735–744
Alonso-Blanco C, Goicoechea PG, Roca A, Alvarez E, Giraldez R, (1994) Genetic mapping of cytological and isozyme markers on chromosomes 1R, 3R, 4R and 6R of rye. Theor Appl Genet 88:208–214
Burham CR (1962) Discussions in cytogenetics. Burgess Publishing Co., Minneapolis
Figueiras AM, González-Jaén MT, Candela M (1990) Chromosomal identification and meiotic behaviour of reciprocal translocations in a rye polymorphic population. Evolutionary implications. Theor Appl Genet 79:686–692
Gill KS, Gill BS, Endo TR (1993) A chromosome region-specific mapping strategy reveals gene-rich telomeric ends in wheat. Chromosoma 102:374–381
Gillies CB (1981) Electron microscopy of spread maize pachytene synaptonemal complexes. Chromosoma 83:575–591
Gillies CB (1985) An electron microscopic study of synaptonemal complex formation at zygotene in rye. Chromosoma 92:165–175
Gillies CB, Cowan SK (1985) The pachytene synaptonemal complex of the cat. Genetica 67:99–107
Gillies CB, Lukaszewski AJ (1989) Synaptonemal complex formation in rye (Secale cereale) heterozygous for telomeric C-bands. Genome 32:901–907
Giraldez R, Cermeño MC, Orellana J (1979) Comparison of C-banding pattern in the chromosomes of inbred lines and open pollinated varieties of rye. Z Pflanzenzuecht 83:40–48
Jones GH, Azkue D de (1993) Synaptonemal complex karyotyping: an appraisal based on a study of Crepis capillaris. Chrom Res 1:197–203
Jong JH de, van Eden J, Sybenga J (1989) Synaptonemal complex formation and metaphase-I configuration patterns in a translocation heterozygote of rye (Secale cereale). Genome 32:72–81
Kaebling M, Fechheimer NS (1983) Synaptonemal complexes and the chromosome complement of domestic fowl, Gallus domesticus. Cytogenet Cell Genet 53:87–92
Kota RS, Gill KS, Gill BS (1993) A cytogenetically-based physical map of chromosome 1B in common wheat. Genome 36:548–554
Lebion G, Zickler D, Lebilcot S (1986) Most UV-induced reciprocal translocations in Sordaria macrospora occur in or near centromere regions. Genetics 112:183–204
Linde-Laursen IB (1988) Giemsa C-banding of barley chromosomes. V. Localization of breakpoints in 70 reciprocal translocations. Hereditas 108:65–76
McClintock B (1930) A cytological demonstration of the location of an interchange between two non-homologous chromosomes of Zea mays. Proc Natl Acad Sci USA 16:791–796
Moses MJ, Counce SJ, Paulson DF (1975) Synaptonemal complex complement of man in spreads of spermatocytes with details of the sex chromosome pair. Science 187:363–365
Moses MJ, Slatton GH, Gambling TM, Starmer CF (1977) Synaptonemal complex karyotyping in spermatocytes of the Chinese hamster (Cricetelus griseus). Chromosoma 60:345–375
Naranjo T, Roca A, Goicoechea PG, de Jong JH, Smilde WD (1989) Comparison between Synaptonemal complexes at pachytene and chromosome association at metaphase-I in heterozygotes for a “non-reciprocal” translocation of rye. Genome 32:983–991
Sherman JD, Stack SM (1992) Two-dimensional spreads of synaptonemal complexes from solanaceous plants. V. Tomato (Lycopersicon esculentum) karyotype and idiogram. Genome 35:354–359
Solari AJ (1980) Synaptonemal complexes and associated structures in microspread human spermatocytes. Chromosoma 81:315–337
Sybenga J, Wolters AHG (1972) The classification of the chromosomes of rye (Secale cereale L.): a translocation tester set. Genetica 43:453–464
Sybenga J, Parmar S, van Eden J, Shewry P (1990) Mapping seed storage-protein loci Sec-1 and Sec-3 in relation to five chromosomal rearrangements in rye (Secale cereale L.). Theor Appl Genet 79:201–208
Vries JM de, Sybenga J (1976) Identification of rye chromosomes: the Giemsa banding pattern and the translocation tester set. Theor Appl Genet 48:35–43
Wandall A, Svendsen A (1983) The Synaptonemal complex karyotype from spread spermatocytes of a dipteran (Aedes aegypty). Can J Genet Cytol 25:361–369
Werner J, Endo TR, Gill BS (1992) Toward a cytogenetically-based physical map of the wheat genome. Proc Natl Acad Sci USA 89:11307–11311
Zickler D, Moreau PJF, Huynh AD, Slezee AM (1992) Correlation between pairing initiation site, recombination nodules and meiotic recombination in Sordaria macrospora. Genetics 132:135–148
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Alvarez, E., Alonso-Blanco, C., Roca, A. et al. Physical mapping of translocation breakpoints in rye by means of synaptonemal complex analysis. Theoret. Appl. Genetics 89, 33–41 (1994). https://doi.org/10.1007/BF00226979
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DOI: https://doi.org/10.1007/BF00226979