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A detailed linkage map around an apple scab resistance gene demonstrates that two disease resistance classes both carry the V f gene

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Abstract

A detailed genetic map has been constructed in apple (Malus x domestica Borkh.) in the region of the v f gene. This gene confers resistance to the apple scab fungus Venturia inaequalis (Cooke) Wint. Linkage data on four RAPD (random amplified polymorphic DNA) markers and the isoenzyme marker PGM-1, previously reported to be linked to the v f gene, are integrated using two populations segregating for resistance to apple scab. Two new RAPD markers linked to v f (identified by bulked segregant analysis) and a third marker previously reported as being present in several cultivars containing v f are also placed on the map. The map around v f now contains eight genetic markers spread over approximately 28 cM, with markers on both sides of the resistance gene. The study indicates that RAPD markers in the region of crab apple DNA introgressed with resistance are often transportable between apple clones carrying resistance from the same source. Analysis of co-segregation of the resistance classes 3A (weakly resistant) and 3B (weakly susceptible) with the linked set of genetic markers demonstrates that progeny of both classes carry the resistance gene.

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Authors and Affiliations

  1. Batchelar Research Centre, Private Bag 11 030, Palmerston North, New Zealand

    S. E. Gardiner & H. C. M. Bassett

  2. Havelock North Research Centre, Private Bag 1401, Havelock North, New Zealand

    D. A. M. Noiton, V. G. Bus, M. E. Hofstee & A. G. White

  3. The Horticulture and Food Research Institute of New Zealand Ltd, Mt. Albert Research Centre, Private Bag 92 169, Auckland, New Zealand

    R. D. Ball, R. L. S. Forster & E. H. A. Rikkerink

Authors
  1. S. E. Gardiner
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  2. H. C. M. Bassett
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  3. D. A. M. Noiton
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  4. V. G. Bus
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  5. M. E. Hofstee
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  6. A. G. White
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  7. R. D. Ball
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  8. R. L. S. Forster
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  9. E. H. A. Rikkerink
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Additional information

Communicated by H. K. Dooner

This work was supported in part by grants from the New Zealand Foundation for Research Science and Technology (FoRST) Programme 94-HRT-07-366 and ENZA New Zealand (International)

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Gardiner, S.E., Bassett, H.C.M., Noiton, D.A.M. et al. A detailed linkage map around an apple scab resistance gene demonstrates that two disease resistance classes both carry the V f gene. Theoret. Appl. Genetics 93, 485–493 (1996). https://doi.org/10.1007/BF00417939

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  • DOI: https://doi.org/10.1007/BF00417939

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