Summary
Exogenous glucagon or epinephrine were infused into normal overnight fasted dogs to raise circulating hormone levels to concentrations within the physiologic range. Plasma levels of glycerol and free fatty acids remained unchanged during the glucagon infusion, but rose significantly during the administration of epinephrine. Plasma insulin in the systemic circulation remained unchanged during the glucagon infusion and increased slightly during the infusion of the catecholamine. The data suggest that in normal dogs glucagon in physiological amounts has no lipolytic effect. The importance of the sympathetic nervous system in regulating lipolysis in normal mammals is stressed.
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Björntorp, P., Schersten, T.: Effect of β-hydroxybutyrate on lipid mobilization. Amer. J. Physiol.212, 683–687 (1967)
Cahill, G.F., Jr., Herrera, M.G., Morgan, A.P., Soeldner, J.S., Steinke, J., Levy, P.L., Reichard, G.A., Jr., Kipnis, D.M.: Hormone-fuel interrelationship during fasting. J. clin. Invest.45, 1751–1969 (1966)
Cherrington, A., Vranić, M., Fono, P., Kovačević, N.: Effect of glucagon on glucose turnover and plasma free fatty acids in depancreatized dogs maintained on matched insulin infusions. Can. J. Physiol.50, 946–954 (1972)
Dole, V.P.: A relation between non-esterified fatty acids in plasma and the metabolism of glucose. J. clin. Invest.35, 150–154 (1956)
Gerich, J., Karam, J., Forsham, P.: Stimulation of glucagon secretion by epinephrine in man. J. clin. Endocr.37, 479–481 (1973)
Gerich, J.E., Langlois, M., Schneider, V., Karam, J.H., Noacco, C.: Effects of alterations of plasma free fatty acid levels on pancreatic glucagon secretion in man. J. clin. Invest.53, 1284–1289 (1974)
Gerich, J.E., Lorenzi, M., Bier, D.M., Tsalikian, E., Schneider, V., Karam, J.H., Forsham, P.H.: Effect of physiologic levels of glucagon and growth hormone on human carbohydrate and lipid metabolism — studies involving administration of exogenous hormone during suppression of endogenous hormone secretion with somatostatin. J. clin. Invest.57, 875–884 (1976)
Hagen, J.H.: Effect of glucagon on the metabolism of adipose tissue. J. biol. Chem.236, 1023–1027 (1961)
Huffman, W.S.: A rapid photoelectric method for the determination of glucose in blood and urine. J. biol. Chem.120, 51–55 (1937)
Madison, L.L., Seyffert, W.A., Unger, R.H., Barker, B.: Effect of plasma free fatty acids on plasma glucagon and serum insulin concentrations. Metabolism17, 301–304 (1968)
Muller, W.A., Faloona, G.R., Unger, R.H.: Hyperglucagonemia in diabetic ketoacidosis. Its prevalence and significance. Amer. J. Med.54, 52–57 (1973)
Muller, W.A., Aoki, T.T., Cahill, G.F., Jr.: Effect of alanine and glycine on glucagon secretion in postabsorptive and fasting man. J. clin. Endocr.40, 418–425 (1975)
Muller, W.A., Aoki, T.T., Flatt, J.P., Blackburn, G.L., Egdahl, R.H., Cahill, G.F. Jr.: Effect of ß-hydroxybutyrate, glycerol and free fatty acid infusions on glucagon and epinephrine secretion in dogs during acute hypoglycemia. Metabolism25, 1077–1086 (1976)
Lefèbvre, P.: The physiologic effect of glucagon on fat metabolism. Diabetologia2, 130–132 (1966)
Liljenquist, J.E., Bomboy, J.D., Lewis, S.B., Sinclair-Smith, B., Felts, P.W., Lacy, W.W., Crofford, O.B., Liddle, G.W.: Effects of glucagon on lipolysis and ketogenesis in normal and diabetic man. J. clin. Invest.53, 190–197 (1974)
Lindsey, C.A., Faloona, G.R., Unger, R.H.: Plasma glucagon in nonketotic hyperosmolar coma. J. Amer. med. Ass.229, 1771–1773 (1974)
Luyckx, A.S., Lefèbvre, P.J.: Arguments for a regulation of pancreatic glucagon secretion by circulating plasma free fatty acids. Proc. Soc. exp. Biol. (N. Y.)133, 524–528 (1970)
Robertson, R.P., Porte, D. Jr.: Adrenergic modulation of basal insulin secretion in man. Diabetes22, 1–8 (1973)
Schade, D.S., Eaton, R.P.: Glucagon regulation of plasma ketone body concentration in human diabetes. J. clin. Invest.56, 1340–1344 (1975)
Seyffert, W.A., Madison, L.L.: Physiologic effects of metabolic fuels on carbohydrate metabolism. I. Acute effect of elevation of plasma free fatty acids on hepatic glucose output, peripheral glucose utilisation, serum insulin and plasma glucagon levels. Diabetes16, 765–776 (1967)
Sherwin, R.S., Fisher, M., Hendler, R., Feiig, P.: Glucagon and glucose regulation in normal, obese and diabetic subjects. New Engl. J. Med.294, 455–460 (1976)
Soeldner, J.S., Slone, D.: Critical variables in the radioimmunoassay of serum insulin using the double antibody technique. Diabetes14, 771–779 (1965)
Trout, D.L., Estes, E.H. Jr., Friedberg, S.J.: Titration of free fatty acids of plasma; a study of current methods and a new modification. J. Lipid Res.1, 199–202 (1960)
Unger, R.H., Aguilar-Parada, E., Muller, W.A., Eisentraut, A.M.: Studies of pancreatic alpha-cell function in normal and diabetic subjects, J. clin. Invest.49, 837–848 (1970)
Unger, R.H., Orci, L.: The essential role of glucagon in the pathogenesis of diabetes mellitus. Lancet1975 I, 14–16
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Supported in part by U.S.P.H.S. grants AM-05077, AM-15191, AM-17456, G.M.-17366, D.A.D.A.-17-67-C-7149 and Swiss National Science Foundation Grant 3.1060.73
Work carried out during the tenure at the E. P. Joslin Research Laboratory
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Muller, W.A., Aoki, T.T., Egdahl, R.H. et al. Effects of exogenous glucagon and epinephrine in physiological amounts on the blood levels of free fatty acids and glycerol in dogs. Diabetologia 13, 55–58 (1977). https://doi.org/10.1007/BF00996328
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DOI: https://doi.org/10.1007/BF00996328