Summary
1. Blood glucose, immunoreactive, insulin suppressible and non-suppressible insulin-like activities were determined in rats after the administration of glucose and D-mannoheptulose respectively. The latter caused a marked rise of the blood sugar concentration There was a correlation between blood sugar and suppressible insulin-like activity in all groups of rats except those injected with mannoheptulose. The results support the concept that mannoheptulose acts by inhibiting the release of insulin from theβ-cells of the islets.-2. Immunoreactive insulin roughly paralleled suppressible ILA, but the quantitative correlation between the two was not satisfactory.-3. Nonsuppressible ILA was approximately the same in all groups of rats. Changes in the level of suppressible ILA were not reflected in the concentration of non-suppressible ILA.
Résumé
1. La glycémie, l'insuline immunoréactive et les activités insuliniques supprimable et non supprimable ont été mesurées chez des rats chez les quels une hyperglycémie avait été provoquée par le glucose ou le D-mannoheptulose. Une corrélation entre la glycémie et l'activité insulinique supprimable a été observée dans tous les groupes de rats à l'exception de ceux traités par le mannoheptulose. Ces résultats confirment l'hypothèse selon laquelle l'action hyperglycémiante du mannoheptulose est due à son pouvoir inhibiteur de la sécrétion d'insuline par les cellules B des îlots de Langerhans.-2. Bien que les valeurs des activités insuliniques immunoréactive et supprimable fussent semblables, la corrélation quantitative entre ces deux mesures ne s'est pas avérée satisfaisante.-3. L'activité insulinique non supprimable est restée la même dans tous les groupes de rats. Les fluctuations de l'activité insulinique supprimable ne se sont pas traduites par des fluctuations de la concentration de l'activité insulinique non supprimable.
Zusammenfassung
1. Blutglucose, immunoreaktives Insulin, hemmbare und nicht hemmbare Insulinaktivität (IA) wurden bei Ratten bestimmt, die mit Glucose oder D-Mannoheptulose hyperglykämisch gemacht wurden. Blutglucose und hemmbare IA korrelierten in allen Gruppen mit Ausnahme der mit Mannoheptulose behandelten Ratten. Diese Resultate bestätigen frühere Befunde und Hypothesen, wonach Mannoheptulose die Insulinsekretion derβ-Zellen der Inseln unterdrückt.-2. Obschon sich immunoreaktives Insulin und hemmbare IA in den verschiedenen Gruppen von Ratten ähnlich verhielten, ergab sich keine gute Korrelation zwischen diesen beiden Größen.-3. Die Konzentration der nicht hemmbaren IA war in allen Gruppen von Ratten ungefähr gleich. Die Veränderungen der hemmbaren IA spiegelten sich in der Konzentration der nichthemmbaren IA nicht wieder.
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Supported by research grants from the Schweizerische Nationalfonds (3854) and from the U.S. Public Health Service (A 5387).
Supported by the Schweizerische Nationalfonds (4042).
Supported in part by U.S. Public Health Service research grant AM 05701-03.
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Froesch, E.R., Jakob, A., Zahnd, G.R. et al. Immunoreactive insulin, suppressible, and non suppressible insulinlike activities in rats after administration of glucose and induction of hyperglycaemia by mannoheptulose. Diabetologia 2, 265–268 (1966). https://doi.org/10.1007/BF01268184
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DOI: https://doi.org/10.1007/BF01268184