Summary
Circulating islet cell antibodies (ICA) were present in high frequency (80%) early after diagnosis and decreased in the time course of childhood diabetes mellitus. The complement fixing ability of islet cell antibodies (CF-ICA) in the course of the disease appeared to depend on the titre of ICA: the coefficient of correlation between ICA and CF-ICA titres was 0.79 and all ICA's with a titre over 16 were complement-fixing. Incubating fresh frozen human pancreatic sections thrice rather than once with the children's sera, increased the detectability of complement fixation by a factor 1.4 in all ICA-positive sera. Thus tested, the detection of complement fixation per se did not appear to have a separate pathogenic significance, as the fraction of complement fixing ICA's was almost constant throughout the clinical course. The presence of ICA-IgG subclasses also was dependent on the ICA titre: above a titre of 16 mostly all four subclasses could be detected. Incubating the pancreatic tissue thrice rather than once with ICA-positive sera resulted in enhanced detectability of ICA-IgG1. Early in the course of childhood diabetes, including two prediabetic children, most of the IgG subclasses could be detected in ICA, but after a duration of one year IgG1 alone was mainly seen. In two other children, having a family history of insulin-dependency, restriction to the IgG2 subclass was found.
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Gorsuch AN, Spencer KM, Lister J, Wolf E, Bottazzo GF, Cudworth AG (1982) Can future Type 1 diabetes be predicted? A study of families of affected children. Diabetes 31: 862–867
Van de Winkel M, Smets G, Gepts W, Pipeleers D (1982) Islet cell surface antibodies from insulin-dependent diabetics bind specifically to pancreatic B cells. J Clin Invest 70: 41–49
Lernmark A, Hägglöf B, Freedman ZR, Irvine WJ, Ludvigsson J, Holmgren G (1981) A prospective analysis of antibodies reactive with pancreatic islet cells in insulin-dependent diabetic children. Diabetologia 20: 471–474
Marner B, Lernmark A, Nerup J, Molenaar JL, Tuk CW, Bruining GJ (1983) Analysis of islet cell antibodies on sections of human pancreas. Diabetologia 25: 93–96
Estes D, Atra E, Peltier A (1973) An immunofluorescent method for the detection of antigamma-globulin antibodies. Arthritis Rheum 16: 1–9
Van der Giessen M, Freyee W, Rossouw E, van Loghem E (1973) Qualitative and quantitative studies on IgG2 globulins in individual human sera with an antiserum capable of differentiating between Gm (n+) and Gm (n-) proteins. Clin Exp Immunol 14: 127–132
Von dem Borne AEGK, Helmerhorst FM, van Leeuwen EF, Peels HG, van Riesz E, Engelfriet CP (1980) Autoimmune thrombocytopenia: detection of platelet antibodies with the suspension immunofluorescence test. Br J Haematol 45: 319–327
Horai S, Class FHJ, van Rood JJ (1981) Detection of platelet antibodies by enzyme linked immunosorbent assay (Elisa). Comparative studies with the indirect immunofluorescence assay. Immunol Lett 3: 155–158
Dean BM, Bottazzo GF, Cudworth AG (1983) IgG-subclass distribution in organ specific autoantibodies. The relationship to complement-fixing ability. Clin Exp Immunol 52: 61–66
Huen AHJ, Haneda M, Freedman Z, Lernmark A, Rubenstein AH (1983) Quantitative determination of islet cell surface antibodies using 125I-protein A. Diabetes 32: 460–465
Baekesshov S, Nielsen JH, Marner B, Bilde T, Ludvigsson J, Lernmark A (1982) Autoantibodies in newly diagnosed diabetic children immunoprecipitate human pancreatic islet cell proteins. Nature 298: 167–169
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Bruining, G.J., Molenaar, J., Tuk, C.W. et al. Clinical time-course and characteristics of islet cell cytoplasmatic antibodies in childhood diabetes. Diabetologia 26, 24–29 (1984). https://doi.org/10.1007/BF00252258
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DOI: https://doi.org/10.1007/BF00252258