Abstract
Oxygen-haemoglobin dissociation curves were determined on dogs' blood using a modified and miniaturised dissociation curve analyser. The Bohr factor describes the way in which pH varies the PO2 corresponding to a particular oxyhaemoglobin concentration. The factor was similar for three saturations and was little affected by whether the pH was changed by changing PCO2, or by adding fixed acid or alkali. The haemoglobin saturations in the mid-range tended to be lower than those predicted by the equation of Rossing and Cain (1966)
Similar content being viewed by others
References
Clerbaux TH, Frans A, Detry JM, Rousseau M (1976) Drift in the oxygen content measured with the LexO2con: long term assessment. J Lab Clin Invest 87:717
Dennis RC, Valeri CR (1980) Measuring percent oxygen saturation of hemoglobin, percent carboxyhemoglobin and methemoglobin, and concentrations of total hemoglobin and oxygen in blood of man, dog and baboon. Clin Chem 26:1304
Duvelleroy MA, Buckles RG, Rosenkaimer S, Tung C, Laver MB (1970) An oxyhemoglobin dissociation analyser. J Appl Physiol 28:227
Garby L, Robert M, Zaar B (1972) Proton- and carbamino-linked oxygen affinity of normal human blood. Acta Physiol Scand 84:482
Hahn CEW, Foex P, Raynor M (1976) A development of the oxyhemoglobin dissociation curve analyser. J Appl Physiol 41:259
Hlastala MP, Woodson RD (1983) Bohr effect data for blood gas calculations. J Appl Physiol 55:1002
Hlastala MP (1979) Physiological significance of the interaction of oxygen and carbon dioxide in blood. Crit Care Med 7:374
Kusumi CR, Butts WC, Ruff WL (1973) Superior analytical performance by electronic cell analysis of blood oxygen conte. Physiologie 35:299
Reeves RB, Park JS, Lapennas GN, Olszowka AJ (1982) Oxygen affinity and Bohr coefficients of dog blood. J Appl Physiol 53:87
Rossing RG, Cain SM (1966) A nomogram relating PO2, pH temperature and hemoglobin saturation in the dog. J Appl Physiol 21:195
Selman BJ, White YS, Tait AR (1975) An evaluation of the LexO2con oxygen content analyser. Anaesthesia 30:206
Teisseire B, Teisseire L, Lautier A, Herigault R, Laurent D (1975) A method of continuous recording on microsamples of the Hb−O2 association curve I. Technique and direct registration of standard results. Bull Physio-Pathol Respir 11:837
Valeri CR, Zaroulis CG, Marchionni L, Patti KJA (1972) A simple method for measuring oxyten content in blood. J Lab Clin Med 79:1035
Van Slyke DA, Neill JM (1924) The determination of gases in blood and other solutions by vacuum extraction and nemometric measurements. J Biol Chem 61:523
Wranne B, Woodson RD, Detter JC (1972) Bohr effect: interaction between H+, CO2 and 2,3-DPG in fresh and stored blood. J Appl Physiol 32:749
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sutton, L., Hahn, C.E.W. & Black, A.M.S. Performance of a miniaturised O2−Hb dissociation curve analyser on dog's blood. Intensive Care Med 13, 131–137 (1987). https://doi.org/10.1007/BF00254799
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00254799