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Investigation of C-phycoerythrin from the cyanobacterium Pseudanabaena W 1174

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Abstract

A cyanobacterium which produces high amounts of C-phycoerythrin was classified as a new Pseudanabaena strain. This strain (number W 1173 of our collection) has been cultivated for 6 years without changing its properties. It resembles Pseudanabaena catenata (strain B 1464-1) morphologically but differs in the pigmentation. Contrary to strain B 1464-1, no chromatic adaptation was observed with strain W 1173. It was found that phycoerythrins from both strains differ in the following properties: isoelectric points, number of bilin chromophores, and immunochemical properties. Besides native C-phycoerythrin (PEI, λmax = 558 nm), a degradation product (PEII, λmax = 544 nm and 562nm) has been found in crude extracts from strain W 1173. Criteria for integrity of C-phycoerythrin were discussed which are essential if this biliprotein is used as taxonomic character.

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Abbreviations

PE:

C-Phycoerythrin

SDS:

sodiumdodecylsulfate

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Authors and Affiliations

  1. Botanisches Institut der Universität Müchen, Menzinger Straße 67, D-8000, München 19, Federal Republic of Germany

    Wolfhart Rüdiger, Rudolf Wagenmann & Günther Muckle

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  1. Wolfhart Rüdiger
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  2. Rudolf Wagenmann
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  3. Günther Muckle
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Dedicated to Professor Dr. O. Kandler on the occasion of his 60th birthday

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Rüdiger, W., Wagenmann, R. & Muckle, G. Investigation of C-phycoerythrin from the cyanobacterium Pseudanabaena W 1174. Arch. Microbiol. 127, 253–257 (1980). https://doi.org/10.1007/BF00427201

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  • DOI: https://doi.org/10.1007/BF00427201

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