Abstract
The elemental composition of gamma particles in zoospores ofAllomyces macrogynus andBlastocladiella emersonii was determined by use of energy dispersive X-ray analysis of glutaraldehyde fixed, thin section zoospores. Isolated preparations of gamma particles were also examined. Gamma particles contained no detectable elements. Similar sized, globular, electron dense cytoplasmic inclusions contained phosphorus and calcium. We suggest that previous studies assigning calcium and phosphorus to gamma particles may have been based on confusion of these two types of cytoplasmic inclusions.
This is a preview of subscription content, log in via an institution to check access.
References
Barstow WE (1979) Ultrastructure of the formation of gamma particles during zoosporogenesis inAllomyces macrogynus andCatenaria anguillulae. Exp Mycol 3:28–44
Barstow WE, Lovett JS (1974) Apical vesicles and microtubules in rhizoids ofBlastocladiella emersonil: Effects of actinomycin D and cycloheximide on development during germination. Protoplasma 82:103–118
Barstow WE, Lovett JS (1975) Formation of gamma particles during zoosporogenesis inBlastocladiella emersonii. Mycologia 67:518–529
Barstow WE, Pommerville J (1980) The ultrastructure of cell wall formation and of gamma particles during encystment ofAllomyces macrogynus zoospores. Arch Microbiol 128:179–189
Burke DD, Barstow WE (1981) Chitin synthase activity inAllomyces mitospores. Abstr. 81st Annu Meet Am Soc Microbiol p 112
Cantino EC, Horenstein EA (1956) Gamma and the cytoplasmic control of differentiation inBlastocladiella. Mycologia 48:443–446
Dalley NE, Sonneborn DR (1982) Evidence thatBlastocladiella emersonii zoospore chitin synthetase is located at the plasma membrane. Biochim Biophys Acta 686:65–76
Hohn T, Lovett JS, Bracker CE (1984) Purification and analysis of the major gamma-particle proteins fromBlastocladiella zoospores. Exper Mycol (in press)
Hutchinson TE, Cantino ME, Cantino EC (1977) Calcium is a prominent constituent of the gamma particle in the zoospore ofBlastocladiella emersonii as revealed by X-ray microanalysis. Biochem Biophys Res Comm 74:336–342
Lange L, Olson LW (1976) The zoospore ofOlpidium brassicae. Protoplasma 90:33–45
Machlis L (1953) Growth and nutrition of water molds in the subgenusEuallomyces. I. Growth factor requirements. Amer J Bot 40:189–195
Mills GL, Cantino EC (1978) The lipid composition of theBlastocladiella emersonii gamma particle and the function of gamma particle lipid in chitin formation. Exp Mycol 2:99–109
Mills GL, Cantino EC (1981) Chitosome-like vesicles from gamma particles ofBlastocladiella emersonii synthesize chitin. Arch Microbiol 130:72–77
Morrison PJ (1977) Gametangial development inAllomyces macrogynus. I. The ultrastructure of early stages of development. Arch Microbiol 113:163–172
Myers RB, Cantino EC (1974) The gamma particle: a study of cell-organelle interaction in the development of the water moldBlastocladiella emersonii. In: Wolsky A (ed) Monographs in developmental biology, vol 3. Karger, Basel, pp 1–117
Wool SH, Held AA (1976) Polyphosphate granules in encysted zoospores ofRozella allomycis. Arch Microbiol 108:145–148
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Aldrich, H.C., Barstow, W.E. & Lingle, W.L. Elemental analysis of particles in fungal zoospores. Arch. Microbiol. 139, 102–104 (1984). https://doi.org/10.1007/BF00692722
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00692722