Abstract
Cells of Rhodotorula gracilis grown for 6 hrs at 2 mm Hg O2 pressure when compared with cells grown for 6 hrs at 140 mm Hg, show: a) a large decrease in the level of ergosterol, b) a significant increase in the level of oleic acid and a decrease in the levels of linoleic and linolenic acids, both in the fatty acid fraction of the phospholipids and in the free fatty acids and neutral fat fractions. The results suggest that the dehydrogenation of oleic acid to linoleic acid is preferentially inhibited at low O2 pressure. The possibility is considered that these changes of lipid metabolism might be causally related with decrease of the growth rate observed at low O2 pressure.
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Albrink, H. J.: The microtitration of total fatty acids of serum with notes on the estimation of triglycerides. J. Lipid Res. 1, 53–59 (1959)
Barber, E. D., Lands, W. E. M.: Quantitative measurement of the effectiveness of unsaturated fatty acids required for the growth of Saccharomyces cerevisiae. J. Bact. 115, 543–551 (1973)
Beffagna, N., Cocucci, S., Cocucci, M., Cocucci, M. C.: Change of the main cells constituents during the growth of Rhodotorula gracilis cultured in liquid medium. Ann. Microbiol. 22, 119–130 (1972)
Brown, C. M., Rose, A. H.: Fatty acid composition of Candida utilis as affected by growth, temperature and dissolved oxygen tension. J. Bact. 99, 371–378 (1969)
Cocucci, M.: The effects of complete anaerobiosis on the metabolism of Rhodotorula gracilis. Ann. Micr. 22, 63–70 (1972)
Cocucci, M., Cocucci, M. C., Marré, E.: Growth inhibition without ATP decrease in Rhodotorula gracilis cultured under oxygen pressure. Plant Science Letters 1, 425–431 (1973)
Cocucci, M., Rossi, G., Vandoni, T.: Adaptation of the mitochondrial systems of Rhodotorula gracilis to low oxygen pressure. Cell Diff. (in press, 1975)
Fujimori, A., Hosoya, N.: Phospholipids in human young placenta. J Biochem. 59, 332–339 (1966)
Gornall, A. G., Bardawill, C. J., David, M. M.: Determination of serum proteins by means of the biuret method. J. Biol. Chem. 177, 751–766 (1949)
Jollow, D., Kellerman, G. M., Linnane, A. W.: The biogenesis of mitochondria. III. The lipid composition of aerobically and anaerobically grown Saccharomyces cerevisiae as related to the membrane systems of the cells. J. biol. Chem. 37, 267–279 (1968)
McMurrough, I., Rose, A. H.: Effects of temperature on the fatty acid composition of Candida utilis. J. Bact. 107, 753–758 (1971)
Morpurgo, G., Serlupi-Crescenzi, G., Tecce, G., Valente, P., Venettacci, D.: Influence of ergosterol on the physiology and the ultrastructure of Saccharomyces cerevisiae. Nature (Lond.) 29, 897–899 (1964)
Pedersen, T. A.: Lipid formation in Cryptococcus terricolus. III. Extraction and purification of lipids. Acta chem. scand. 16, 374–382 (1962)
Roger, R., Ma, T. S.: Diazomethan as reagent for microsynthesis. Microchem. J. 1, 245–247 (1957)
Rogers, P. J., Stewart, P. R.: Mitochondrial and peroxisomal contributions to the energy metabolism of Saccharomyces cerevisiae in continuous culture. J. gen. Microbiol. 79, 205–217 (1973)
Rottem, S., Cirillo, V. P., de Kruyff, B., Shinitzky, M., Razin, S.: Correlation of enzymic and transport activities with physical state of lipids in membranes of Mycoplasma mycoides var. capri adapted to grow with low cholesterol concentrations. Biochim. biophys. Acta (Amst.) 323, 509–519 (1973a)
Rottem, S., Yoshou, J., Ne'eman, Z., Razin, S.: Cholesterol in Mycoplasma membranes. Composition, ultrastructure and biological properties of membranes from Mycoplasma mycoides var. capri cells adapted to grow with low cholesterol concentration. Biochim. biophys. Acta (Amst.) 323, 495–508 (1973b)
Siekevitz, P.: Uptake of radioactive alanine in vitro into proteins of rat liver fractions. J. biol. Chem. 195, 459–595 (1962)
Stadtman, T. C.: Preparation and assay of cholesterol and ergosterol. In: Methods in enzymology, Vol. III, S. P. Colowick, N. O. Kaplan, eds., p. 392. New York: Academic Press 1957
Vignais, P. M., Nachbaur, J., Het, J., Vignais, V.: Studies in the phospholipids of yeast mitochondria. Biochem. J. 116, 42 P (1970)
Zlatoust, M., Balabanov, A.: Effects of steroid compounds on the growth of Saccharomyces cerevisiae culture. Isv. Akad. Nauk. Mold. SSR, Ser. Biol. Khim. Nauk. 6, 88–94 (1971)
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Cocucci, M.C., Belloni, G. & Gianani, L. Oxygen pressure, fatty acid composition and ergosterol level in Rhodotorula gracilis . Arch. Microbiol. 105, 17–20 (1975). https://doi.org/10.1007/BF00447106
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DOI: https://doi.org/10.1007/BF00447106