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Cell wall polysaccharides from Talaromyces species

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Abstract

The neutral sugars and amino sugars, released by acid hydrolysis of walls and polysaccharidic fractions, of six species of Talaromyces and the infrared spectra have been used to study their interspecific relationships. In whole cell walls neutral sugars ranged from 23 to 39.6% dry weight and were identified as glucose, galactose and mannose. Glucosamine varied from 8 to 19.8% in the samples. Galactosamine (2% or less) was found in T. emersonii and T. rotundus and no galactosamine in the other species. Sequential fractionation of the cell walls with alkali and acid gave several polysaccharidic fractions. The main differences among species were found in the alkali-soluble fraction at 20° (F1). This fraction represented 8 to 33.2% of the whole cell wall and was characterized as an α-glucan in T. bacillisporus, T. emersonii, T. luteus and T. rotundus (Group A) and as a β-galactofuranosyl containing glucan in T. ohiensis and T. stipitatus (Group B). The alkali-insoluble residue (F4) represented the bulk of the cell wall in all species tested (33.2% to 57.3%) and was characterized as a β-glucan/chitin complex. The results may indicate degrees of interspecific relationship in the genus Talaromyces.

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Abbreviations

CWM:

cell wall material

GLC:

gas-liquid chromatography

IR:

infrared

wt:

weight

CBS:

Centraal Bureau voor Schimmelcultures (Baarn. The Netherlands)

Ara:

arabinose

Xyl:

xylose

Man:

mannose

Gal:

galactose

Glc:

glucose

GlcNH2 :

glucosamine

GalNH2 :

galactosamine

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Authors and Affiliations

  1. Centro de Investigaciones Biológicas, C.S.I.C., Velázquez 144, E-28006, Madrid, Spain

    P. Rupérez, A. Moya & J. A. Leal

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  1. P. Rupérez
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  2. A. Moya
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  3. J. A. Leal
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Rupérez, P., Moya, A. & Leal, J.A. Cell wall polysaccharides from Talaromyces species. Arch. Microbiol. 146, 250–255 (1986). https://doi.org/10.1007/BF00403225

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  • DOI: https://doi.org/10.1007/BF00403225

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