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Classification of staphylococci based on chemical and biochemical properties

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Summary

In the present work the chemical cell wall composition and some other biochemical characteristics were studied in staphylococci with the intention of utilizing the data obtained in their classification.

According to the cell wall peptidoglycans and teichoic acids, the 130 strains of staphylococci studied are divided into 10 major groups. This division of staphylococci into groups is in good agreement with their present classification only in some cases. All of the 47Staphylococcus aureus strains contain a cell wall peptidoglycan of thel-Lys-Gly5–6 type and ribitol teichoic acid. Coagulase-negative staphylococci are more heterogeneous and are divided according to their cell wall composition into 9 major groups. 21 strains of them are classified asS. epidermidis sensu stricto. They form a natural group and are distinguished by the occurrence of thel-Lys-Gly4–5,l-Ser0.5–1.8 peptidoglycan type, glycerol teichoic acid and anl-lactate dehydrogenase which is activated by fructose-1,6-diphosphate. 8 strains with peptidoglycan of thel-Lys-Gly4–5,l-Ser0.5–1.8 type and ribitol teichoic acid are labeled asS. saprophyticus. The remaining groups have not been given species names and require further extensive comparative study.

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Authors and Affiliations

  1. Botanisches Institut der Universität München, München, Germany

    K. H. Schleifer

  2. Czechoslovak Collection of Microorganisms, Brno

    M. Kocur

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  1. K. H. Schleifer
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  2. M. Kocur
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Schleifer, K.H., Kocur, M. Classification of staphylococci based on chemical and biochemical properties. Archiv. Mikrobiol. 93, 65–85 (1973). https://doi.org/10.1007/BF00666081

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