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Substanz P in der Retina

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Summary

1. The substance P content of cow retinae has been estimated by comparing the extract from the retina with two different standard preparations (SP-S, SP-B) extracted from cow gut. The bioassay on various preparations (guinea-pig ileum, rat duodenum, hen coecum, rabbit blood pressure) gave different substance P equivalents. The standards prepared from the gut are therefore of limited sufficiency for estimating substance P in nervous tissue.

The substance P content of retina was not altered by environmental light.

2. Both substance P standards (SP-S, SP-B) contained several active fractions after separation by paper- and column chromatography. The retina extract contained only one fraction corresponding to Zetler's fraction Fa.

3. The highest substance P content per mg protein has been found in the subfraction M2 which also contained the highest concentration of cholineacetylase when subcellular particles have been separated according to the method of Whittaker.

4. Separation of subcellular particles of the retina by the de Robertis method showed the highest smooth muscle stimulating activity in the “synaptic vesicle fraction” M2. The slow type of contraction produced by this fraction, however, differed from the faster type of contraction produced by the standards or the brain extracts containing substance P.

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Literatur

  • Barsoum, G. S., and J. H. Gaddum: The pharmacological estimation of adenosine and histamine in blood. J. Physiol. (Lond.) 85, 1 (1935).

    Google Scholar 

  • Cleugh, J.: zit. nach V. P. Whittaker: Pharmacological studies with isolated cell components. Biochem. Pharmacol. 9, 61 (1962).

    Google Scholar 

  • Condon, N. E.: A modification of the conventional mercury manometer for blood pressure recordings. Brit. J. Pharmacol. 6, 19 (1951).

    Google Scholar 

  • Euler, U. S. von: Herstellung und Eigenschaften von Substanz P. Acta physiol. scand. 4, 373 (1942).

    Google Scholar 

  • — Substance P in subcellular particles in peripheral nerves. Ann. N. Y. Acad. Sci. 104, 449 (1963).

    Google Scholar 

  • --, and F. Lishajko: Presence of substance P in subcellular particles of peripheral nerves. Symposium on Substance P, Sarajevo 1961.

  • Gaddum, J. H.: The estimation of substance P in tissue extracts. Symposium on Substance P, p. 7, Sarajevo 1961.

  • Horton, E. W.: Human urinary kinin excretion. Brit. J. Pharmacol. 14, 125 (1959).

    Google Scholar 

  • Hebb, C. O., and V. P. Whittaker: Intracellular distribution of acetylcholine and cholinacetylase. J. Physiol. (Lond.) 142, 187 (1958).

    Google Scholar 

  • Kocic-Mitrovic, D.: Über die Wirkungen einiger physiologischer Reize auf die Konzentration der Substanz P in Gehirn und Retina. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 236, 37 (1959).

    Google Scholar 

  • Laszlo, I.: Enzymic removal of Adenosine-5-phosphate (AMP) from substance P extracts. J. Physiol. (Lond.) 153, 69 (1960).

    Google Scholar 

  • Lembeck, F.: Zur Frage der zentralen Übertragung afferenter Impulse. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 219, 197 (1953).

    Google Scholar 

  • —, u. A. Holasek: Die intracelluläre Lokalisation der Substanz P. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 238, 542 (1960).

    Google Scholar 

  • —, and G. Zetler: Substance P: a polypeptide of possible significance, especially within the nervous system. Int. Rev. Neurobiol. 4, 159 (1962).

    Google Scholar 

  • Palladin, A. V.: Distribution of enzymes among the various subcellular units of the brain. Regional Neurochemistry, p. 8. Oxford: Pergamon Press 1961.

    Google Scholar 

  • Robertis, E. de, A. Pellegrinonde Iraldi, G. Rodriguez de Lores Arnaiz, and I. Salganicoff: Cholinergic and non-cholinergic nerve endings in rat brain. J. Neurochem. 9, 23 (1962).

    Google Scholar 

  • — G. Rodriguez de Lores Arnaiz, I. Salganicoff, A. de Irlaldi, and L. M. Zieher: Isolation of synaptic vesicles and structural organization of the acetylcholine system within brain nerve endings. J. Neurochem. 10, 225 (1963).

    Google Scholar 

  • Ryall, R. W.: Subcellular distribution of pharmacologically active substances in guinea pig brain. Nature (Lond.) 196, 680 (1962).

    Google Scholar 

  • Smallman, B. N.: Choline acetylase activity of rabbit brain. J. Neurochem. 2, 119 (1958).

    Google Scholar 

  • Toh, C. G.: Biologically active substances in brain extracts. J. Physiol. (Lond.) 165, 47 (1963).

    Google Scholar 

  • Vogler, K., W. Haefely, A. Hürlimann, R. O. Studer, W. Lergier, R. Strässle, and K. H. Berneis: A new purification procedure and biological properties of substance P. Ann. N. Y. Acad. Sci. 104, 378 (1963).

    Google Scholar 

  • Whittaker, V. P.: The isolation and characterization of Acetylcholine containing particles from brain. Biochem. J. 72, 694 (1959).

    Google Scholar 

  • — Pharmacological studies with isolated cell components. Biochem. Pharmacol. 9, 61 (1962).

    Google Scholar 

  • Zetler, G.: Zwei neue pharmakologisch aktive Polypeptide in einem Substanz P-haltigen Extrakt. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 242, 330 (1961).

    Google Scholar 

  • —, u. G. Ohnesorge: Die Substanz P-Konzentration im Gehirn bei verschiedenen Funktionszuständen des Zentralnervensystems. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 231, 199 (1957).

    Google Scholar 

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Authors and Affiliations

  1. Pharmakologisches Institut der Universität Tübingen, Germany

    H. Held, A. Heizmann, F. Lembeck & W. Schlote

Authors
  1. H. Held
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  2. A. Heizmann
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  3. F. Lembeck
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  4. W. Schlote
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Held, H., Heizmann, A., Lembeck, F. et al. Substanz P in der Retina. Naunyn - Schmiedebergs Arch 253, 246–259 (1966). https://doi.org/10.1007/BF00246375

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  • DOI: https://doi.org/10.1007/BF00246375

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