Summary
Recent reports indicate that the presynaptic negative feedback mechanism involved in regulating sympathetic neurotransmission is only functional within a narrow range of neuronal activity. The specific aim of this report is to examine the release of sympathetic transmitter at varying frequencies of stimulation, and the effects of α-adrenoceptor antagonists on the overflow. The experiments were carried out in the3H-noradrenaline-labeled heart of the guinea pig. The overflow of tritium (per pulse) increased from a stimulation frequency of 0.125 to 10 Hz and declined at 30 Hz. Phentolamine or yohimbine produced facilitation of the overflow from 0.125 to 10 Hz; the effect was more pronounced at lower frequencies of stimulation. We conclude that the presynaptic α-adrenoceptor-mediated negative feedback mechanism operates over a wide range of cardiac sympathetic nerve activity.
This is a preview of subscription content, log in via an institution to check access.
References
Bell C, Vogt M (1971) Release of endogenous noradrenaline from an isolated muscular artery. J Physiol (Lond) 215: 509–520
Brown GL, Gillespie JS (1957) The output of sympathetic transmitter from the spleen of the cat. J Physiol (Lond) 138: 81–102
Cubeddu LX, Weiner N (1975) Nerve stimulation-mediated overflow of norepinephrine and dopamine-β-hydroxylase. III. Effects of norepiphrine depletion on the alpha presynaptic regulation of release. J Pharmacol Exp Ther 192: 1–14
Haefely W, Huerlimann A, Thoenen H (1965) Relation between the rate of stimulation and the quantity of noradrenaline liberated from sympathetic nerve endings in the isolated perfused spleen of the cat. J Physiol (Lond) 181: 48–58
Kirpekar SM, Misu Y (1967) Release of noradrenaline by splenic nerve stimulation and its dependence on calcium. J Physiol (Lond) 188: 219–234
Löffelholz K, Muscholl E (1969) A muscarinic inhibition of the noradrenaline release evoked by postganglionic sympathetic nerve stimulation. Naunyn-Schmiedeberg's Arch Exp Pathol Pharmakol 265: 1–15
Langer SZ (1980) Presynaptic regulation of the release of catecholamines. Pharmacol Rev 32: 337–362
Langer SZ, Dubocovich ML, Celuch SM (1975) Prejunctional regulatory mechanisms for noradrenaline release elicited by nerve stimulation. In: Almgren O, Carlsson A, Engel J (eds) Chemical tools in catecholamine research, vol 2. North Holland, Amsterdam Oxford, pp 183–191
Starke K (1972) Influence of extracellular noradrenaline on the stimulation-evoked secretion of noradrenaline from sympathetic nerves. Evidence for an α-receptor-mediated feedback inhibition of noradrenaline release. Naunyn-Schmiedeberg's Arch Exp Pathol Pharmakol 275: 11–23
Starke K, Endo T, Taube H (1975a) Pre- and postsynaptic components in effect of drugs with α-adrenoceptor affinity. Nature 254: 440–441
Starke K, Borowski E, Endo T (1975b) Preferential blockade of presynaptic alpha-adrenoceptors by yohimbine. Eur J Pharmacol 34: 358–388
Stjärne L (1973) Alpha-adrenoceptor-mediated feedback control of sympathetic neurotransmitter secretion in guinea-pig vas deferens. Nature, New Biol 241: 190–191
Stjärne L, Brundin J (1977). Frequency-dependence of3H-noradrenaline secretion from human vasoconstrictor nerves: Modification by factors interfering with α and β-adrenoceptor or prostaglandin E2-mediated control. Acta Physiol Scand 101: 199–210
Story DF, McCulloch WM, Rand MJ, Standford-Starr CA (1981) Conditions required for the inhibitory feedback loop in noradrenergic transmission. Nature 293: 62–65
Trendelenburg U (1980) A kinetic analysis of the extraneuronal uptake and metabolism of catecholamines. Rev Physiol Biochem Pharmacol 87: 33–115
Wakade AR, Wakade TD (1978) Inhibition of noradrenaline release by adenosine. J Physiol (Lond) 282: 35–49
Wakade AR, Wakade TD (1981) Release of noradrenaline by one pulse: Modulation of such release by alpha-adrenoceptor antagonists and uptake blockers. Naunyn-Schmiedeberg's Arch Pharmacol 317: 302–309
Author information
Authors and Affiliations
Additional information
This material is based upon work supported in part by The National Science Foundation Grant #BNS79-23019, and in part by the National Institutes of Health Grant #HL-18601
Rights and permissions
About this article
Cite this article
Wakade, A.R., Wakade, T.D. Does presynaptic regulation of sympathetic transmission occur within a limited range of neuronal activity?. Naunyn-Schmiedeberg's Arch. Pharmacol. 321, 77–79 (1982). https://doi.org/10.1007/BF00586354
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00586354