Summary
The release of endogenous dopamine evoked by electrical stimulation or by exposure to (+)-amphetamine (10 μM) was determined in superfused striatal slices of the rat.
The spontaneous and the electrically-evoked release of dopamine were significantly increased in the presence of nomifensine (10 μM). After reserpine pretreatment (5 mg/kg, s.c., 24 h), the striatal dopamine content was reduced by about 90%. Exposure to 10 μM (+)-amphetamine during 2 min released similar amounts of dopamine from striatal slices of untreated or reserpine pretreated rats. Similar results were obtained when monoamine oxidase activity was inhibited in vivo with pargyline.
Pretreatment with reserpine does not modify the (+)-amphetamine-induced release of dopamine, in spite of the marked reduction of the striatal dopamine content. These results provide direct evidence for the view that (+)-amphetamine releases dopamine from a special, reserpine-resistant pool of newly synthetized transmitter.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arbilla S, Langer SZ (1980) Influence of monoamine oxidase inhibition on the release of 3H-dopamine elicited by potassium and by amphetamine from the rat substantia nigra and corpus striatum. Naunyn-Schmiedeberg's Arch Pharmacol 311:45–52
Arbilla S, Dennis T, Langer SZ, Niddam R, Scatton B (1984a) Pretreatment with reserpine does not modify the amphetamine induced release of endogenous dopamine in striatal slices. Br J Pharmacol 82:249P
Arbilla S, Baud P, Cantrill R, Langer SZ (1984b) Endogenous phenolamines: do they have a direct postsynaptic action on dopamine receptors in the central nervous system? Br J Pharmac 82:252P
Baud P, Arbilla S, Langer SZ (1984) Inhibition of the electricaly-evoked release of 3H-acetylcholine in rat striatal slices: an experimental model for drugs that enhance dopaminergic neurotransmission. J Neurochem (in press)
Boulton AA, Juorio AV (1982) Brain trace amines. In: Lajtha A (ed) Chemical and cellular architecture. Plenum Press, New York and London, pp 189–222
Cantrill R, Arbilla S, Langer SZ (1983a) Inhibition by d-amphetamine of the electrically-evoked release of [3H]acetylcholine from slices of the rat striatum: involvement of dopamine receptors. Eur J Pharmacol 87:167–168
Cantrill R, Arbilla S, Zivkovic B, Langer SZ (1983b) Amphetamine enhances latent dopaminergic neurotransmission in the rat striatum: effects on 3H-acetylcholine release. Naunyn-Schmiedeberg's Arch Pharmacol 322:322–324
Cubeddu LX, Hofmann IS (1983) Frequency-dependent release of acetylcholine and dopamine from rabbit striatum: its modulation by dopaminergic receptors. J Neurochem 41:94–101
Da Prada M, Zürcher G (1976) Simultaneous radioenzymatic determination of plasma and tissue adrenaline, noradrenaline and dopamine within the femtomole range. Life Sciences 19:1161–1174
Hertting G, Zumstein A, Jackisch R, Hoffmann I, Starke K (1980) Modulation by endogenous dopamine of the release of acetylcholine in the caudate nucleus of rabbit. Naunyn-Schmiedeberg's Arch Pharmacol 315:111–117
Kamal L, Arbilla S, Langer SZ (1981) Presynaptic modulation of the release of dopamine from the rabbit caudate nucleus: differences between electrical stimulation, amphetamine and tyramine. J Pharmac Exp Ther 216:592–598
Kamal L, Arbilla S, Galzin AM, Langer SZ (1983) Amphetamine inhibits the electrically evoked release of 3H-dopamine from slices of the rabbit caudate. J Pharmac Exp Ther 227:446–458
Langer SZ, Arbilla S (1984) The amphetamine paradox in dopaminergic neurotransmission. Trends Pharmacol Sci 5:387–390
Lehmann J, Langer SZ (1983) The striatal cholinergic interneuron: synaptic target of dopaminergic terminals? Neuroscience 10:1105–1120
Lehmann J, Lee CR, Langer SZ (1983) Dopamine receptors modulating 3H-acetylcholine release in slices of the cat caudate: effects of (−)-N-(2-chloroethyl)-norapomorphine. Eur J Pharmacol 90:393–400
Lowry OH, Rosebourgh NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Miller JC, Friedhoff AJ (1979) Effects of haloperidol and apomorphine on the K+-depolarized overflow of 3H-dopamine from striatal slices. Biochem Pharmacol 28:688–690
Moore KE (1977) The actions of amphetamine on neurotransmitters: a brief review. Biological Psychiatry 12:451–462
Scatton B (1982) Effect of dopamine agonists and neuroleptic agents on striatal acetylcholine transmission in the rat: evidence against dopamine receptors multiplicity. J Pharmacol Exp Ther 220:197–202
Semerdjian-Rouquier L, Bossi L, Scatton B (1981) Determination of 5-hydroxytryptophan, serotonin and 5-hydroxindoleacetic acid in rat and human brain and biological fluids by reversed-phase high-performance liquid chromatography with electrochemical detection. J Chromatography 218:663–670
Starke K, Reimann W, Zumstein A, Hertting G (1978) Effect of dopamine receptor agonists and antagonists on release of dopamine in the rabbit caudate nucleus in vitro. Naunyn-Schmiedeberg's Arch Pharmacol 305:27–36
Thornburg JE, Moore KE (1973) The relative importance of dopaminergic and noradrenergic neuronal systems for the stimulation of locomotor activity induced by amphetamine and other drugs. Neuropharmacol 12:853–866
Uretsky NJ, Kamal L, Snodgrass SR (1979) Effect of divalent cations on the amphetamine-induced stimulation of [3H]-catechol synthesis in the striatum. J Neurochem 32:951–960
Uretsky NJ, Snodgrass SR (1977) Studies on the mechanism of stimulation of dopamine synthesis by amphetamine in striatal slices. J Pharmacol Exp Ther 202:565–580
Zumstein A, Karduck W, Starke K (1981) Pathways of dopamine metabolism in the rabbit caudate nucleus in vitro. Naunyn-Schmiedeberg's Arch Pharmacol 316:205–217
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Niddam, R., Arbilla, S., Scatton, B. et al. Amphetamine induced release of endogenous dopamine in vitro is not reduced following pretreatment with reserpine. Naunyn-Schmiedeberg's Arch. Pharmacol. 329, 123–127 (1985). https://doi.org/10.1007/BF00501200
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00501200