Summary
The perivascular nerves of rabbit mesenteric arteries were stimulated with 15 pulses at 2 Hz, and decreases in external diameter were measured by means of a photoelectric device. Both extra- and intraluminally added [Met5]-enkephalin 1 μmol/l depressed vasoconstriction, although with the second mode of application a larger inhibition occurred. Therefore, in the subsequent experiments all opioids were added into the lumen. [Met5]enkephalin 0.1 μmol/l had no effect. [d-Pen2, l-Pen5]enkephalin 3 μmol/l was less potent than [Met5]enkephalin 1 μmol/l. ICI 174864 1 μmol/l was also without effect when given alone, but antagonized the action of [Met5]enkephalin 1 μmol/l.Ethylketocyclazocine, dynorphin A(1–13), normorphine and DAGO, all 1 μmol/l, were ineffective. [Met5]enkephalin 1 μmol/l did not change the vasoconstriction evoked by the application of noradrenaline (0.1 –3 μmol/l). It is concluded that in the mesenteric artery action potential-induced transmitter release, and in consequence vasoconstriction can be inhibited by the activation of presynaptic opioid δ-receptors.
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Cotton R, Giles MG, Miller L, Shaw JS, Timms D (1984) ICI 174864; a highly selective antagonist for the opioid δ-receptor. Eur J Pharmacol 97:331–332
Ensinger H, Hedler L, Szabo B, Starke K (1986) Bremazocine causes sympatho-inhibition and hypotension in rabbits by activating peripheral κ-receptors. J Cardiovasc Pharmacol 8:470–475
Illes P, Bettermann R, Brod I, Bucher B (1987) β-Endorphinsensitive opioid receptors in the rat tail artery. Naunyn-Schmiedeberg's Arch Pharmacol (in press)
Illes P, Pfeiffer N (1985) Regulation of blood pressure by peripheral opioid mechanisms. In: Bevan JA, Godfraind T, Maxwell RA, Stoclet JC, Worcel M (eds) Vascular neuroeffector mechanisms. Elsevier, Amsterdam, pp 175–180
Illes P, Pfeiffer N, von Kügelgen I, Starke K (1985) Presynaptic opioid receptor subtypes in the rabbit ear artery. J Pharmacol Exp Ther 232:526–533
Illes P, Ramme D, Starke K (1986) Presynaptic opioid δ-receptors in the rabbit mesenteric artery. J Physiol 379:217–228
Kosterlitz HW (1985) Opioid peptides and their receptors. Proc Roy Soc Lend B 225:27–40
Kummer W, Reinecke M, Heym C, Forssmann WG (1987) Distribution of opioid peptides functionally related to the cardiovascular system. In: Stumpe KO, Faden AI, Kraft K (eds) Opioid peptides and blood pressure control. Springer, Berlin Heidelberg New York Tokyo (in press)
Lord JAH, Waterfield AA, Hughes J, Kosterlitz HW (1977) Endogeneous opioid peptides: multiple agonists and receptors. Nature 267:495–499
Mosberg HI, Hurst R, Hruby VJ, Gee K, Yamamura HI, Galligan JJ, Burks TIT (1983) Bis-penicillamine enkephalins possess highly improved specificity towards δ opioid receptors. Proc Natl Acad Sci USA 80:5871–5874
Paterson SJ, Robson LE, Kosterlitz HW (1983) Classification of opioid receptors. Br Med Bull 39:31–36
Ramme D, Illes P, Späth L, Starke K (1986) Blockade of α2-adrenoceptors permits the operation of otherwise silent opioid κ-receptors at the sympathetic axons of rabbit jejunal arteries. Naunyn-Schmiedeberg's Arch Pharmacol 334:48–55
Schabert A, Bauer RD, Busse R (1980) Photoelectric device for the recording of diameter changes of opaque and transparent blood vessels in vitro. Pflügers Arch 385:239–242
Seelhorst A, Starke K (1986) Prejunctional opioid receptors in the pulmonary artery of the rabbit. Arch Int Pharmacodyn 281:298–310
Szabo B, Hedler L, Ensinger H, Starke K (1986) Opioid peptides decrease noradrenaline release and blood pressure in the rabbit at peripheral receptors. Naunyn-Schmiedeberg's Arch Pharmacol 332:50–56
von Kügelgen I, Illes P, Wolf D, Starke K (1985) Presynaptic inhibitory opioid δ- and κ-receptors in a branch of the rabbit ileocolic artery. Eur J Pharmacol 118:97–105
Wallenstein S, Zucker CL, Fleiss JL (1980) Some statistical methods useful in circulation research. Circ Res 47:1–9
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Illes, P., Ramme, D. & Busse, R. Photoelectric measurement of neurogenic vasoconstriction in jejunal branches of the rabbit mesenteric artery reveals the presence of presynaptic opioid δ-eceptors. Naunyn-Schmiedeberg's Arch Pharmacol 335, 701–704 (1987). https://doi.org/10.1007/BF00166990
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DOI: https://doi.org/10.1007/BF00166990