Summary
The influence of the alphalb-adrenoceptor-selective antagonist chlorethylclonidine on the alpha1-adrenergic positive inotropic effect and the phosphoinositide hydrolysis induced by phenylephrine was investigated in the rabbit ventricular myocardium. Pretreatment of membrane fractions derived from the rabbit ventricular muscle with 10−5 mol/l chlorethylclonidine decreased the specific binding of [3H]prazosin (at a saturating concentration of 10−9 mol/l) from the control value of 11.27±0.48 to 4.18±1.87 fmol/mg protein. The inhibition by adrenaline of the binding of [3H]prazosin (slope factor and affinity) was not affected by chlorethylclonidine. The positive inotropic effect of phenylephrine (in the presence of 3 × 10−7 mol/l bupranolol) was inhibited by chlorethylclonidine in a concentration-dependent manner (10−7−10−5 mol/l) and abolished by 10−5 mol/l chlorethylclonidine. The concentration of chlorethylclonidine to inhibit the phenylephrine-induced maximum response to 50% was 2.4 × 10−6 mol/l. The accumulation of [3H]inositol monophosphate and [3H]inositol trisphosphate induced by 10−5 mol/l phenylephrine was inhibited by chlorethylclonidine in the same concentration range. These findings indicate that the myocardial alpha1-adrenoceptors mediating a positive inotropic effect in the rabbit ventricular myocardium may belong to the chlorethylclonidine-sensitive alpha1b-subtype, and that the subcellular mechanism of action involve phosphoinositide hydrolysis.
References
Abdel-Latif AA (1986) Calcium mobilizing receptors, polyphosphoinositides, and the generation of second messengers. Pharmacol Revs 38:227–272
Apkon M, Nerbonne JM (1988) α1-Adrenergic agonists selectively suppress voltage-dependent K+ currents in rat ventricular myocytes. Proc Natl Acad Sci USA 85:8756–8760
Berridge MJ, Dawson RMC, Downes CP, Heslop JP, Irvine RF (1983) Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositides. Biochem J 212:473–482
Berridge MJ, Downes CP, Hanley MR (1982) Lithium amplifies agonist-dependent phosphatidylinositol responses in brain and salivary glands. Biochem J 206:587–595
Berridge MJ, Irvine RF (1984) Inositol trisphosphate, a novel second messenger in cellular signal transduction. Nature 312:315–321
Brown JH, Buxton IL, Brunton LL (1985) αl-Adrenergic and muscarinic cholinergic stimulation of phosphoinositide hydrolysis in adult rat cardiomyocytes. Circ Res 57:532–537
Brückner R, Scholz H (1984) Effects of α-adrenoceptor stimulation with phenylephrine in the presence of propranolol on force of contraction, slow inward current and cyclic AMP content in the bovine heart. Br J Pharmacol 82:223–232
Endoh M (1986) Regulation of myocardial contractility via adrenoceptors: differential mechanisms of α- and β-adrenoceptor-mediated actions. In: Grobecker H, Philippu A, Starke K (eds) New aspects of the role of adrenoceptors in the cardiovascular system. Springer, Berlin Heidelberg New York, pp 78–105
Endoh M, Blinks JR (1988) Actions of sympathomimetic amines on the Ca2+ transients and contractions of rabbit myocardium: reciprocal changes in myofibrillar responsiveness to Ca2+ mediated through α- and β-adrenoceptors. Circ Res 62:247–265
Endoh M, Hiramoto T, Ishihata A, Takanashi M, Inui J (1991) Myocardial α1-adrenoceptors mediating the positive inotropic effect and changes in phosphatidylinositol metabolism: species difference in receptor distribution and intracellular coupling process in mammalian ventricular myocardium. Circ Res (in press)
Fedida D, Shimoni Y, Giles WR (1989) A novel effect of norepinephrine on cardiac cells is mediated by α1-adrenoceptors. Am J Physiol 256:H1500-H1504
Han C, Abel PW, Minneman KP (1987) α1-Adrenoceptor subtypes linked to different mechanisms for increasing intracellular Ca2+ in smooth muscle. Nature 329:333–335
Hanft G, Gross G, Beckeringh JJ, Korstanje C (1989) α1-Adrenoceptors: the ability of various agonists and antagonists to discriminate between two distinct [3H]prazosin binding sites. J Pharm Pharmacol 41:714–716
Hartmann HA, Mazzocca NJ, Kleiman RB, Houser SR (1988) Effects of phenylephrine on calcium current and contractility of feline ventricular myocytes. Am J Physiol 255:H1173-H1180
Hescheler J, Nawrath H, Tang M, Trautwein W (1988) Adrenoceptor-mediated changes of excitation and contraction in ventricular heart muscle from guinea-pigs and rabbits. J Physiol 397:657–670
Hiramoto T, Kushida H, Endoh M (1988) Further characterization of the myocardial a-adrenoceptors mediating positive inotropic effects in the rabbit myocardium. Eur J Pharmacol 152:301–310
Iwakura K, Hori M, Watanabe Y, Kitabatake A, Cragoe EJ Jr, Yoshida H, Kamada T (1990) αl-Adrenoceptor stimulation increases intracellular pH and Ca2+ in cardiomyocytes through Na+/H+ and Na+/Ca2+ exchange. Eur J Pharmacol 186:29–40
Kushida H, Hiramoto T, Satoh H, Endoh M (1988) Phorbol ester does not mimic, but antagonizes the alpha-adrenoceptor-mediated positive inotropic effect in the rabbit papillary muscle. Naunyn-Schmiedeberg's Arch Pharmacol 337:169–176
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Minneman KP (1988) α1-Adrenergic receptor subtypes, inositol phosphates, and sources of cell Ca2+. Pharmacol Revs 40:87–119
Minneman KP, Han C, Abel PW (1988) Comparison of α1-adrenergic receptor subtypes distinguished by chlorethylclonidine and WB 4101. Mol Pharmacol 33:509–514
Nishizuka Y (1984) The role of protein kinase C in cell surface signal transduction and tumour promotion. Nature 308:693–698
Nishizuka Y (1988) The molecular heterogeneity of protein kinase C and its implications for cellular regulation. Nature 334:661–665
Otani H, Otani H, Das DK (1988) α1-Adrenoceptor-mediated phosphoinositide breakdown and inotropic response in rat left ventricular papillary muscles. Circ Res 62:8–17
Scholz H (1980) Effects of beta- and alpha-adrenoceptor activators and adrenergic transmitter releasing agents on the mechanical activity of the heart. In: Szekeres L (ed) Handbook of experimental pharmacology. Springer, Berlin Heidelberg New York, pp 651–733
Scholz J, Schaefer B, Schmitz W, Scholz H, Steinfath M, Lohse M, Schwabe U, Puurunen J (1988) Alpha-1 adrenoceptor-mediated positive inotropic effect and inositol trisphosphate increase in mammalian heart. J Pharmacol Exp Ther 245:327–335
Schümann HJ, Endoh M, Brodde OE (1975) The time course of the effects of α- and β-adrenoceptor stimulation by isoprenaline and methoxamine on the contractile force and cAMP level of the isolated rabbit papillary muscle. Naunyn-Schmiedeberg's Arch Pharmacol 289:291–302
Simpson PC, Cuenco RG, Paningbatan MO, Murphy MD (1990) An α1-adrenergic receptor subtype sensitive to WB-4101 transduces cardiac myocyte growth. Circulation 82 [Suppl III]:III-561 (abstr)
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Takanashi, M., Norota, I. & Endoh, M. Potent inhibitory action of chlorethylclonidine on the positive inotropic effect and phosphoinositide hydrolysis mediated via myocardial alpha1-adrenoceptors in the rabbit ventricular myocardium. Naunyn-Schmiedeberg's Arch Pharmacol 343, 669–673 (1991). https://doi.org/10.1007/BF00184301
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DOI: https://doi.org/10.1007/BF00184301