Effects of single or multiple choice trials per session on drug discrimination performance

Abstract

Drug discrimination procedures typically provide for multiple choice opportunities per training session. This practice allows non-drug cues (presence or absence of reinforcement) to mediate choice behavior during that portion of the session following the initial choice. In this experiment, rats were trained to discriminate 1.0 mg/kg cyclazocine from saline using a novel procedure that employed a single-choice trial per training session. Drug discrimination acquisition and generalization were compared to those of rats given discrimination training with 30 choice trials per session. The one-trial procedure yielded stable and reliable acquisition but more slowly than did the multiple trials procedure. The one-trial procedure produced longer first trial choice latencies and enhanced the tendency for subjects to respond on both choice levers during the first trial. The cyclazocine generalization functions were comparable, but the one-trial subjects more often responded on both choice levers, particularly when administered intermediate test doses of cyclazocine. Control of choice behavior by the reinforcer cue was evaluated on a mid-session cue reversal test. Multiple-trial subjects persisted in responding on the saline level following a midsession injection of cyclazocine, whereas one-trial subjects shifted to the cyclazocine-appropriate lever.