Summary
Plasma renin could be detected in 16 nl of rat plasma by incubating at 50°C, pH 6.2 with 20 μl of a rat renin substrate. In 60 nl samples it could be estimated and differences in concentration of 20% could be detected.
Plasma renin concentration was measured in blood from the superficial efferent arterioles of a rat's kidney. The concentrations of renin in different efferent arterioles of the same rat were similar. The renin concentration of efferent arteriolar blood was 361±189 (S.D.) ng Al ml−1 (blood) hour−1 which was not different from the renin concentration of simultaneously taken femoral artery blood (340±217).
Plasma renin concentration can be measured in efferent arteriolar blood and will allow the control of renin secretion to be studied.
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Supported by Grants from the NHF and LIMRF of Australia.
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Johnston, C.I., Matthews, P.G., Davis, J.M. et al. Renin measurement in blood collected from the efferent arteriole of the kidney of the rat. Pflugers Arch. 356, 277–286 (1975). https://doi.org/10.1007/BF00583839
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DOI: https://doi.org/10.1007/BF00583839