Summary
The two theoretical models proposed previously to calculate the intracapillary and transcapillary glomerular pressure gradients from the sieving of macromolecules such as PVP have been used to analyse in 22 normotensive dogs the sieving curve relating the sieving coefficients, Φ, to molecular size (Φ: glomerular clearance of PVP fractions/GFR).
Neither the “localc 2” model-filtrate unmixed at the outer face of the capillaries walls—nor the constantc 2 model-filtrate well mixed—allowed to obtain realistic values for the hemodynamical parameters. Indeed with the localc 2 model, the best fit between calculated and experimental sieving curves could be obtained only by reversing the intracapillary pressure gradient; conversely the constantc 2 model obliged to decrease the intracapillary pressure so abruptly along the capillaries, that retrofiltration took place in the distal parts of the vessels.
This difficulty has been overcome by combining the two models; the so-called “hybrid model” considers that the filtrate is well mixed in the vicinity of the urinary pole only.
The following results were obtained: 1. PGCa and PGCe (intracapillary pressures at the afferent and efferent extremities of the capillaries) equal to 49.7±1.03 and 41.8±1.00 mm Hg respectively. 2. Pressure equilibrium is generally reached at the efferent extremity of the vessels. 3. The slope of PGC\(\left( {\frac{{PGC_a - PGC_e }}{{PGC_a }}} \right)\) varies inversely to F.F. (filtration fraction). 4. The model, however, does not allow to rule out the possibility of retrofiltration.
Similar content being viewed by others
References
Andrews, P.: The gel filtration behaviour of protein related to their molecular weight over a wide range. Biochem. J.96, 595–606 (1965)
Arturson, G., Granath, K.: Dextrans as test molecules in studies of the functional ultrastructure of biological membranes. Clin. chim. Acta37, 309–322 (1972)
Blantz, R. C.: Effect of mannitol on glomerular ultrafiltration in the hydropenic rat. J. clin. Invest.54, 1135–1143 (1974)
Bonsnes, R. W., Taussky, H. H.: On the colorimetric determination of creatinine by the Jaffe reaction. J. biol. Chem.158, 581 (1945)
Brenner, B. M., Troy, J. L., Daugharty, T. M., Deen, W. M., Robertson, C. R.: Dynamics of glomerular ultrafiltration in the rat. II. Plasma-flow dependence of GFR. Amer. J. Physiol.223, 1184–1190 (1972)
Decoodt, P., Du Bois, R., Gassée J. P., Verniory, A., Lambert, P. P.: A model for sieving of macromolecules by the glomerular membrane of the kidney. Comp. Progr. in Biomed.4, 180–188 (1975)
Deen, W. M., Robertson, C. R., Brenner, B. M.: A model of glomerular ultrafiltration in the rat. Amer. J. Physiol.223, 1178–1183 (1972)
Deen, W. M., Troy, J. L., Robertson, C. R., Brenner, B. M.: Dynamics of glomerular ultrafiltration in the rat. IV. Determination of the ultrafiltration coefficient. J. clin. Invest.52, 1500–1508 (1973)
Determan, H., Michel, W.: The correlation between molecular weight and elution behaviour in the gel chromatography of proteins. J. Chromatogr.25, 303–313 (1966)
Du Bois, R., Decoodt, P., Gassée, J. P., Verniory, A., Lambert, P. P.: Determination of glomerular intracapillary and transcapillary pressure gradients from sieving data. I. A mathematical model. Pflügers Arch.356, 299–316 (1975)
Gassée, J. P.: Effects of acetylcholine on glomerular sieving of macromolecules. Pflügers Arch.342, 239–254 (1973)
Gassée, J. P.: Hémodynamique glomérulaire et tamisage des macromolécules de polyvinylpyrrolidone. Thèse d'agrégation (1975)
Gassée, J. P., Decoodt, P., Verniory, A., Lambert, P. P.: Autoregulation of effective glomerular filtration pressure. Amer. J. Physiol.226, 616–623 (1974)
Gornall, A. G., Bardawil, C. J., David, M. M. J.: Determination of serum protein by means of biuret reaction. J. biol. Chem.177, 751 (1949)
Hardwicke, J., Hulme, B., Jones, J. H., Ricketts, C. P.: Measurement of glomerular permeability to polydisperse radioactively labelled macromolecules in normal rabbits. Clin. Sci.34, 505–514 (1968)
Harvey, R. B., Brothers, A. J.: Renal extraction of PAH and creatinine measured by continuous in vivo sampling of arterial and renal vein blood. Ann. N. Y. Acad. Sci.102, 46–54 (1962)
Killander, J., Laurent, T. C.: A theory of gel filtration and its experimental verification. J. Chromatogr.14, 317–330 (1964)
Lambert, P. P., Verniory, A., Gassée, J. P., Ficheroulle, P.: Sieving equations and effective glomerular filtration pressure. Kidney Int.2, 131–146 (1972)
Lambert, P. P., Verniory, A., Gassée, J. P., Decoodt, P.: Glomerular sieving of macromolecules and effective filtration pressure. Proc. Vth Intern. Congr. of Nephrology, Mexico 1972, vol. 3, pp. 9–16. Basel: Karger 1974
Putter, A.: Aktive Oberfläche und Organfunktion. Z. allg. Physiol.12, 125–214 (1911)
Renkin, E. M., Gilmore, J. P.: Glomerular filtration. In: Handbook of Physiology Section 8. Renal Physiology. Section. Ed. J. Orloff and R. Berliner, pp. 185–248 Washington, D. C.: Am Physiol. Soc. 1973
Roe, J. H., Epstein, J. H., Goldstein, N. D.: A photometric method for the determination of inulin in plasma and urine. J. biol. Chem.178, 839–845 (1949)
Verniory, A., Du Bois, R., Decoodt, P., Gassée, J. P., Lambert, P. P.: Measurement of the permeability of biological membranes. Application to the glomerular wall. J. gen. Physiol.62, 489–507 (1973)
Author information
Authors and Affiliations
Additional information
This investigation was supported by the National Fund for Medical Research of Belgium, Grant 1197.
Rights and permissions
About this article
Cite this article
Lambert, P.P., Du Bois, R., Decoodt, P. et al. Determination of glomerular intracapillary and transcapillary pressure gradients from sieving data II. A physiological study in the normal dog. Pflugers Arch. 359, 1–22 (1975). https://doi.org/10.1007/BF00581274
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00581274