Skip to main content
SpringerLink
Log in

Single nephron filtration rates (SNGFR) in the trout,Salmo gairdneri

Validation of the use of ferrocyanide and the effects on environmental salinity

  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
  • Published:
Pflügers Archiv Aims and scope Submit manuscript

Abstract

  1. 1.

    Trout kidney slices incubated in the presence of14C-sodium ferrocyanide accumulated radioactivity. Uptake by individual nephroi increased with time, stabilizing after 30 min. The greater the concentration of radioactivity the greater the uptake per mm of tubule, while reduced specific activity inhibited14C uptake.

  2. 2.

    The renal excretory pattern of14C-sodium ferrocyanide was compared with that of3H-inulin in fresh water adapted trout. The renal clearance rates of the substances were similar and linearly related over a range or urine flows, providing plasma ferrocyanide concentrations were between 1.5 and 2.5 mM.14C-ferrocyanide is thus a reliable marker for glomerular activity in this species.

  3. 3.

    A protocol for the determination of single nephron glomerular filtration rates (SNGFR) in trout is described. The method exploits the ability to visualize ferrocyanide as Prussian Blue within nephroi after a single pulse injection superimposed upon a constant infusion of14C-ferrocyanide. The effects of surgical procedure and extraluminal contamination are assessed.

  4. 4.

    The SNGFR of freshwater trout, 1.31±0.2 nl/min is approximately 65% less than that observed in sea water adapted fish (3.74±1.12 nl/min). The overall, total kidney glomerular filtration rate, (GFR) is 142.6±17.4 μl/min/kg in fresh water animals and 20.1±0.9 μl/min/kg in sea water fish.

  5. 5.

    It is concluded that whilst SNGFRs of sea water animals are higher than those of fresh water animals, filtration is distributed to nephron populations selected to meet the homeostatic demands of the organism.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anderson, B. G., Anderson, W. D.: Renal vasculature of the trout demonstrated by scanning electron microscopy compared with canine glomerular vessels. Am. J. Anat.145, 443–458 (1976)

    Google Scholar 

  • Baines, A. D., Rouffignac, C. de: Functional heterogeneity of nephrons. II. Filtration rates intraluminal flow velocities and fractional water reabsorption. Pflügers Arch.308, 260–276 (1969)

    Google Scholar 

  • Bankir, L., Rouffignac, C. de: Anatomical and functional heterogeneity of nephrons in the rabbit: Microdissection studies and SNGFR measurements. Pflügers Arch.336, 89–93 (1976)

    Google Scholar 

  • Berliner, R. W., Kennedy, I. J., Hilton, J. G.: Renal clearance of ferrocyanide in the dog. Am. J. Physiol.160, 325–329 (1950)

    Google Scholar 

  • Bonvalet, J. P., Champion, M., Wanstock, F., Berjal, G.: Nephron filtration rates in conscious rats. Am. J. Physiol.232, F329-F334 (1977)

    Google Scholar 

  • Braun, E. J., Dantzler, W. H.: Function of mammalian-type and reptilian-type nephrons in kidney of desert quail. Am. J. Physiol.222, 616–629 (1972)

    Google Scholar 

  • Brown, J. A.: Development and application of methods for the study of renal function in lower vertebrates. Ph. D. Thesis, University of Sheffield, England (1976)

    Google Scholar 

  • Brown, J. A., Henderson, I. W.: Single nephron filtration rates in the trout,Salmo gairdneri. J. Endocr.67, 63P-64P (1975)

    Google Scholar 

  • Chabardès, D., Poujeol, P., Deiss, S., Bonvalet, J. P., Rouffignac, C. de: Intrarenal glomerular filtration rate distribution in salt loaded rats. Pflügers Arch.349, 191–202 (1974)

    Google Scholar 

  • Coelho, J. B.: Effect of dietary sodium intake on the intrarenal distribution of nephron glomerular filtration rates in the rat. Circ. Res.33, 547–554 (1973)

    Google Scholar 

  • Coelho, J. B., Chien, K. C. H., Stella, S. R., Bradley, S. E.: Relationship of extraluminal tubular deposition of ferrocyanide to peritubular perfusion rate in the cortical and medullary nephron segments of the rat kidney. Circ. Res.29, 21–28 (1971)

    Google Scholar 

  • Eggleton, M. G., Habib, Y. A.: Urmary excretion of ferrocyanide in the cat. J. Physiol. (Lond.)114, 199–207 (1951)

    Google Scholar 

  • Hanssen, O. E.: A histochemical method for evaluation of excreted sodium ferrocyanide in isolated tubules of the mouse kidney. Acta path. microbiol. scand.44, 363–371 (1958)

    Google Scholar 

  • Hickman, C. P.: Glomerular filtration rate and urine flow in the euryhaline southern flounder,Paralichthys lethostigma. Can. J. Zool.46, 427–438 (1968)

    Google Scholar 

  • Hickman, C. P., Trump, B. F.: The kidney. In: Fish physiology. (W. S. Hoar and D. J. Randall, eds.), Vol. 1, pp. 91–240. London-New York: Academic Press 1969

    Google Scholar 

  • Jackson, B. A.: Endocrine and environmental effects on nephron function in lower vertebrates. Ph. D. Thesis, University of Sheffield, England (1977)

    Google Scholar 

  • Jackson, B. A., Brown, J. A., Oliver, J. A., Henderson, I. W.: Actions of angiotensin on single nephron filtration rates of trout,Salmo gairdneri adapted to fresh- and sea-water environments. J. Endocr.75, 32P-33P (1977)

    Google Scholar 

  • Kleeman, C. R., Epstein, F. H.: Fate and distribution of Fe59-labelled ferrocyanide in humans and dogs. Proc. Soc. Exp. Biol. Med.93, 228–233 (1956)

    Google Scholar 

  • Lahlou, B.: La fonction renal des téléostéens et son rôle dans l'osmoregulation. Bull. Infs. tech. Commt. Energ. atom.144, 17–32 (1970)

    Google Scholar 

  • Lamiere, N. H., Lifschitz, M. D., Stein, J. H.: Heterogeneity of nephron function. Ann. Rev. Physiol.39, 159–184 (1977)

    Google Scholar 

  • Marshall, E. K.: Comparison of function of the glomerular and aglomerular kidney. Am. J. Physiol.94, 1–10 (1930)

    Google Scholar 

  • Miller, E. K., Winkler, A.: The ferrocyanide clearance in man. J. clin. Invest.15, 489–492 (1936)

    Google Scholar 

  • Moss, N. G., Rouffignac, C. de: Comparison between the renal handling of14C−Na-ferrocyanide and3H-methoxy-inulin in the rat. A microinjection study. Pflügers Arch.338, 19–29 (1972)

    Google Scholar 

  • Olivereau, M., Olivereau, J.: Effects of transfer to sea water and back to fresh water on the histological structure of the eel kidney. J. comp. Physiol.115, 223–239 (1977)

    Google Scholar 

  • Rouffignac, C. de, Bonvalet, J. P.: Use of sodium ferrocyanide as glomerular indicator to study the functional heterogeneity of nephrons. Yale J. Biol. Med.45, 243–253 (1972)

    Google Scholar 

  • Sawyer, W. H.: Lungfishes and amphibians: endocrine adaptations and transition from aquatic to terrestrial life. Fedn. Proc. Fedn. Am. Socs. exp. Biol.31, 1609–1614 (1972)

    Google Scholar 

  • sykes, A. H.: The excretion of inulin, creatinine and ferrocyanide by the fowl. Res. Vet. Sci.1, 315–320 (1960)

    Google Scholar 

Download references

Author information

Author notes

  1. B. A. Jackson

    Present address: Department of Physiology and Biophysics, Mayo Graduate School of Medicine, 55901, Rochester, Minnesota, U.S.A.

Authors and Affiliations

  1. Department of Zoology, University of Sheffield, S10 2TN, Sheffield, UK

    J. Anne Brown, B. A. Jackson, J. Ann Oliver & I. W. Henderson

Authors
  1. J. Anne Brown
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. A. Jackson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Ann Oliver
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. I. W. Henderson
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Brown, J.A., Jackson, B.A., Oliver, J.A. et al. Single nephron filtration rates (SNGFR) in the trout,Salmo gairdneri . Pflugers Arch. 377, 101–108 (1978). https://doi.org/10.1007/BF00584381

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00584381

Key words

Search

Navigation