Abstract
The technique of orthograde tubular microperfusion has been used to test the functional integrity of tubuloglomerular feedback control in adrenalectomized rats. Kidneys of these animals display high renin contents, as well as an impairment of electrolyte transport in the loop of Henle, which was demonstrated by monitoring the electrical conductivity of fluid entering the distal tubule over a perfusion range of 10–50 nl/min. The calculated electrolyte concentration of the perfusion fluid leaving the loop of Henle was significantly higher in adrenalectomized rats than in controls. Intratubular stop flow pressure (SFP) during perfusion with a modified “endproximal” Ringer's solution was recorded continuously in early proximal segments at rates of 0–40 nl/min. Direct pressure transmission from perfused endproximal tubular segments to the pressure recording capillary was prevented by paraffin blockade of the intermediate proximal segment. An intact feedback response in each of the 32 tubules of the adrenalectomized rats studied was observed, which was reversible and could be demonstrated repetitively in individual tubules. Thus ΔSFP at 40 nl/min was −11.2±1.3 mm Hg in adx rats as compared to −8.1±1.3 in control rats. An increased freedback sensitivity was observed in dexamethasone treated adrenalectomized rats despite normal renal renin content. Here ΔSFP at 40 nl/min was −15±1.1. Thus, the feedback signal can be mediated in the apparent absence of adrenal steroid hormones and a high sensitivity of tubuloglomerular feedback control is not necessarily paralleled by high renin content of renal tissue.
Similar content being viewed by others
Abbreviations
- adx:
-
adrenalectomized
- AI:
-
angiotensin I
- b.w.:
-
body weight
- k:
-
conductivity (Siemens cm−1)
- EPFR:
-
early proximal flow rate
- FFP:
-
free flow pressure
- Ptub :
-
intratubular pressure
- Pā :
-
mean arterial pressure
- SFP:
-
stop, flow pressure
- SNGFR:
-
single nephron glomerular filtration rate
- Vu :
-
urine flow rate
- ø:
-
perfusion rate through the loop of Henle
References
Axelsson J, Wahlström B, Johansson B, Jonsson O (1967) Influence of the ionic environment on spontaneus electrical and mechanical activity of the rat portal vein. Circ Res 21:609–618
Barajas L, Latta H (1963) The juxtaglomerular apparatus in adrenalectomized rats. Light and electronmicroscopic observations. Lab Invest 12:1046–1059
Biamino G, Johansson B (1970) Effect of calcium and sodium on contracture tension in the smooth muscle of the rat portal vein. Pflügers Arch 321:143–158
Bohle A, Tomsche U (1953) Das Verhalten der epitheloiden Zellen der vasa afferentia der Nierenkörperchen bei experimenteller Hypotonie. Beitr Path Anat 113:399–413
Bohr DF (1964) Electrolytes and smooth muscle contraction. Pharmacol Rev 16:85–111
Bohr DF, Seidel C, Sobieski J (1969) Possible role of sodiumcalcium pumps in tension development of vascular smooth muscle. Microvasc Res 1:335–343
Brunkhorst R, Müller-Ott K, Gutsche HU, Niedermayer W (1978) Effect of furosemide, bumetanide and piretanide on the sensor of the tubuloglomerular feedback mechanism. Proc Eur Dial Transplant Assoc 15:613–616
Buchler O, Riedel B, Erkocak A (1967) Die epitheloiden Zellen des juxtaglomerulären Apparates der Meerschweinchenniere vor und nach bilateraler Adrenalektomie. Verh Anat Ges Anat Anz, Arg H 120:243–249
Burke TJ, Navar LG, Clapp JR, Robinson RR (1974) Response of single nephron glomerular filtration rate to distal nephron microperfusion. Kidney Int 6:230–240
Burg M, Stoner L, Cardinal J, Green N (1973) Furosemide effect on isolated perfused tubules. Am J Physiol 225:119–124
Cortney MA, Nagel W, Thurau K (1966) A micropuncture study on the relationship between flow rate through the loop of Henle and sodium concentration in early distal tubule. Pflügers Arch 287:286–295
Crabbé J, Nichols G (1959) Effects of adrenalectomy and aldosterone on sodium concentration in renal medulla of hydropenic rats. Proc Soc Exp Biol 101:168–171
Dunihue FW (1949) The effect of adrenal insufficiency and of desoxycorticosterone acetate on the juxtaglomerular apparatus. Anat Res 103:442–449
Fishman MC (1976) Membrane potential of juxtaglomerular cells. Nature 260:642–644
Fleckenstein A, Grün G, Tritthart M, Byon K (1971) Uterusrelaxation durch hochaktive Ca2+-antagonistische Hemmstoffe der elektromechanischen Kopplung wie Isoptin (Verapamil, Iproveratril), Substanz 600, Segontin (Prenylamin). Klin Wochenschr 49:32–41
Golenhofen K, Lammel E (1972) Selective suppression of some components of spontaneous activity in various types of smooth muscle by iproveratril (verapamil). Pflügers Arch 331:233–243
Gross F, Sulser F (1957) Der Einfluß der Nebennieren auf die blutdrucksteigernde Wirkung von Renin und auf pressorische Substanzen der Niere. Naunyn-Schmiedebergs Arch exp Path Pharmak 230:274–283
Gutsche H-U, Müller-Suur R, Hegel U, Hierholzer K (1980) Electrical conductivity of tubular fluid of the rat nephron: mieropuncture study of the diluting segment in situ. Pflügers Arch 383:113–121
Gutsche HU, Müller-Suur R, Hegel U, Hierholzer K, Lüderitz S (1975) A new method for intratubular blockade in micropuncture experiments. Pflügers Arch 354:197–202
Gutsche HU, Müller-Suur R, Hierholzer K (1975) Direct evaluation of the diluting capacity af ascending limbs in vivo. 6th Int Congr Nephrol Abstr 726 (1975)
Gutsche HU, Beer G, Niedermayer W, Mályusz M (1977) The effect of aldosterone and dexamethasone on the diluting capacity of thick ascending limbs in experimental adrenal insufficiency. Proc Eur Dial Transplant Assoc 14:574–576
Hierholzer K, Wiederholt M, Holzgreve H, Giebisch G, Klose RM, Windhager EE (1965) Micropuncture study of renal transtubular concentration gradients of sodium and potassium in adrenalectomized rats. Pflügers Arch 285:193–210
Hierholzer K, Wiederholt M, Stolte H (1966) Hemmung der Natriumresorption im proximalen und distalen Konvolut adrenalektomierter Ratten. Pflügers Arch 291:43–62
Hierholzer K, Butz M, Müller-Suur R, Lichtenstein I (1972) Pressure measurements in proximal surface tubules of the rat —single nephron filtration rate and tubuloglomerular feedback. Yale J Biol Med 45:224–232
Hierholzer K, Müller-Suur R, Gutsche HU, Butz M, Lichtenstein I (1974) Filtration in surface glomeruli as regulated by flow rate through the loops of Henle. Pflügers Arch 352:315–337
Hierholzer K, Lange S (1974) The effect of adrenal steroids on renal function. MTP-International Review of Science, Physiology Series, Vol 6, Chapt. 9, Butterworth & Co., London
Imai M (1977) Effect of bumetanide and furosemide on the thick ascending limb of Henle's loop of rabbits and rats perfused in vitro. Eur J Pharmacol 41:409–416
Israelit AH, Rector FC Jr, Seldin DW (1973) The influence of perfusate composition and perfusion rate on glomerular capillary hydrostatic pressure. 6th Ann. Meeting, Am Soc Nephrol, Abstr p 53 (Washington DC, 1973)
Kment A, Dahlheim H (1977) Kinetic studies on renin release of superfused kidney cortex slices and microdissected glomeruli. Pflügers Arch 368:R14
Lohfert H, Lichtenstein I, Butz M, Hierholzer K (1971) Continuous measurement of renal intratubular pressures with a combined pressure transducer microperfusion system. Pflügers Arch 327:191–202
Malvin RB, Sullivan LP, Wilde WS (1958) Localization of nephron transport by stop-flow analysis. Am J Physiol 194:135–142
Mason J, Takabatake T, Olbricht C, Thurau K (1978) The early phase of acute renal failure III — tubuloglomerular feedback. Pflügers Arch 373:69–76
Mason J, Kain H, Shiigai T, Welsch J (1980) The early phase of acuterenal failure V — the influence of suppressing the reninangiotensin system. Pflügers Arch (in press)
Morgan T, Berliner RW (1969) A study by continuous microperfusion of water and electrolyte movements in the loop of Henle and distal tubule of the rat. Nephron 6:388–405
Morgan T, Tadokoro M, Martin D, Berliner RW (1970) Effect of furosemide on Na+ and K+ transport studied by microperfusion of the rat nephron. Am J Physiol 218:292–297
Müller-Ott K, Brunkhorst R, Gutsche HU, Niedermayer W (1978) Effect of piretanide on the electrolyte transport of thick ascending limbs of rat nephrons. 7th Int Congr Nephrol Abstr (Montréal, 1978)
Müller-Suur R, Gutsche HU (1978) Effect of intratubular substitution of Na+ and Cl− ions on the operation of the tubuloglomerular feedback. Acta Physiol Scand 103:353–362
Müller-Suur R, Gutsche HU, Samwer KF, Oelkers W, Hierholzer K (1975) Tubuloglomerular feedback in rat kidneys of different renin contents. Pflügers Arch 359:33–56
Müller-Suur R, Gutsche HU, Schurek HJ (1977) Acute and reversible inhibition of tubuloglomerular feedback mediated afferent vasoconstriction by calciumantagonist verapamil. In: Schmidt U, Dubach U (eds) Current problems in clinical biochemistry 6, “Renal metabolism in relation to renal function”. Hans Huber Publishers. Bern
Murayama Y, Suzuki A, Tadokoro M, Sakai F (1968) Microperfusion of Henle's loop in the kidneys of the adrenalectomized rat. Jpn J Pharmacol 18:518–519
Nasjletti A, Masson GMC (1968) Effects of corticoids on plasma angiotensinogen and renin activity. Am J Physiol 217:1396–1400
Navar GL, Burke TJ, Robinson RR, Clapp JR (1974) Distal tubular feedback and the autoregulation of single nephron glomerular filtration rate. J Clin Invest 53:516–525
Navar LG, Bell PD (1977) Distal tubulo-glomerular feedback response to alterations in distal nephron perfusate flow and composition in the dog. Proc Int Union Physiol Sci Vol XII, Abstr No. 353 (Paris 1977)
Persson AEC, Müller-Suur R, Selén G Peritubular capillary oncotic pressure as a modifier of tubuloglomerular feedback. Am J Physiol (submitted for publication)
Persson AEG, Schnermann J, Wright FS (1974) Effect of isotonic volume expansion of feedback control of glomerular filtration rate. Proc Int Union Physiol Sci Vol XI, p 119 (New Delhi 1974)
Peters G (1959) Aufnahme und renale Ausscheidung von Wasser und Salzen bei freiem Nahrungs- und Trinkflüssigkeitsangebot; ibre Beeinflussung durch Adrenalektomie und Behandlung mit Nebennierenrindenhormonen bei der Ratte. Naunyn-Schmiedebergs Arch exp Path Pharmak 235:205–229
Ploth DW, Schnerman J, Dahlheim H, Hermle M, Schmidmeier E (1977) Autoregulation and tubuloglomerular feedback in normotensive and hypertensive rats. Kidney Int 12:253–267
Sakai F, Murayama Y (1971) Effect of aldosterone and cortisol on Henle's loop in the adrenalectomized rat's kidney. Jpn J Pharmacol 21:23–31
Samwer KF, Schreiber M, Molzahn M, Oelkers W (1974) Pressor effect of angiotensin II in sodium replete and deplete rats. Relationship to plasma renin, plasma sodium and hematocrite before and after ganglionic blockade. Pflügers Arch 346:307–318
Schaechtelin G, Regoli D, Gross F (1963) Bio-assay of circulating renin-like pressor material by isovolemic cross circulation. Am J Physiol 205:303–306
Schnermann J, Horster M, Levine DZ (1969) The influence of sampling technique on the micropuncture determination of GFR and reabsorptive characteristics of single rat proximal tubules. Pflügers Arch 309:48–58
Schnermann J, Wright FS, Davis JM, Stackelberg W, Grill G (1970) Regulation of superficial nephron filtration rate by tubulo-glomerular feedback. Pflügers Arch 318:147–175
Schnermann J, Persson AEG, Agerup B (1973) Tubuloglomerular feedback. Nonlinear relation between glomerular hydrostatic pressure and loop of Henle perfusion rate. J Clin Invest 52:862–869
Schnermann J, Hermle M, Schmidmeier E, Dahlheim H (1975) Impaired potency for feedback regulation of glomerular filtration rate in DOCA escaped rats. Pflügers Arch 358:325–338
Schnermann J, Ploth DW, Hermle M (1976) Activation of tubuloglomerular feedback by chloride transport. Pflügers Arch 326:229–240
Somlyo AP, Somlyo AV (1968) Electromechanical and pharmacomechanical coupling in vascular smooth muscle. J Pharmacol Exp Ther 159:129–145
Somlyo AP, Somlyo AV (1968) Vascular smooth muscle. I. Normal biochemistry and biophysic. Pharmacol Rev 20:197–272
Somlyo AP, Somlyo AV (1970) Vascular smooth muscle. II. Pharmacology of normal and hypertensive vessels. Pharmacol Rev 22:249–353
Sigler MH, Forrest JN, Elkington JR (1965) Renal concentration ability in the adrenalectomized rat. Clin Sci 28:29–37
Steinhausen M (1963) Eine Methode zur Differenzierung proximaler und distaler Tubuli der Nierenrinde von Ratten in vivo und ihre Anwendung zur Bestimmung tubulärer Strömungsgeschwindigkeiten. Pflügers Arch 277:23–35
Stolte H, Brecht JP, Wiederholt M, Hierholzer K (1967) Influence of adrenalectomy and glucocorticoid hormones on the water permeability of superficial nephron segments in the rat kidney. Pflügers Arch 299:99–127
Thurau K (1964) Renal hemodynamics. Am J Med 36:698–719
Thurau K, Schnermann J (1965) Die Natriumkonzentration an der Macula densa als regulierender Faktor für das Glomerulumfiltrat (Mikropunktionsversuche). Klin Wochenschr 48:410–413
Thurau K, Dahlheim H, Grüner A, Mason J, Granger P (1972) Activation of renin in the single juxtaglomerular apparatus by sodium chloride in the tubular fluid at the macula densa. Circ Res Suppl 30/31, II, 182–186
Wright FS, Schnermann J (1974) Interference with feedback control of glomerular filtration rate by furosemide, triflocine and cyanide. J Clin Invest 53:1695–1708
Wright FS, Briggs JP (1977) Feedback regulation of glomerular filtration rate. Am J Physiol 233:F1–F7
Author information
Authors and Affiliations
Additional information
Supported by the Deutsche Forschungsgemeinschaft (Hi 97/14)
Rights and permissions
About this article
Cite this article
Gutsche, HU., Müller-Suur, R., Samwer, K.F. et al. Tubuloglomerular feedback control in kidneys of adrenalectomized rats. Pflügers Arch. 386, 11–19 (1980). https://doi.org/10.1007/BF00584181
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00584181