Summary
Adult Munich Wistar rats undergoing mild salt diuresis (NaCl 20 g·l−1, 0.1 ml·min−1) were injected with tracer doses of3H-Inulin and32P-sodium phosphate in thin descending and ascending limbs of Henle's loop, collecting ducts accessible at the surface of the papilla and early distal superficial tubules. Kidneys were prepared for simultaneous papillary microinjection and urinary flow collection. Expressed in percent of the amounts injected, unidirectional phosphate reabsorption fluxes were 5±1% and 3±1% for injections into early distal superficial tubules and collecting ducts, respectively. By contrast, the flux was 21.7±3% for injections into either the descending or ascending thin limbs of juxtamedullary nephrons. We conclude from these results that in the rat, a significant amount of phosphate is reabsorbed by the juxtamedullary distal tubules and/or the subsequent arcades connecting the juxtamedullary distal tubules to the collecting ducts.
Similar content being viewed by others
References
Amiel C, Kuntziger HE, Richet G (1970) Micropuncture study of handling of phosphate by proximal and distal nephron in normal and parathyroidectomized rat. Evidence for distal reabsorption. Pflügers Arch 317:93–109
Bengele HH, Lechene CP, Alexander EA (1979) Phosphate transport along the inner medullary collecting duct of the rat. Am J Physiol 237:F48–F54
Brunette MG, Taleb L, Carriere S (1973) Effect of parathyroid hormone on phosphate reabsorption along the nephron of the rat. Am J Physiol 225:1076–1081
Chen PS, Toribara TY, Warner H (1964) Microdetermination of phosphorus. Anal Chem 28:1756–1758
Dennis VW, Bello-Reuss E, Robinson R (1977) Response of phosphate transport to parathyroid hormone in segments of rabbit nephron. Am J Physiol 233:F29–F38
Goncharevskaya OA, Dlouha H (1975) The development of various generations of nephrons during postnatal ontogenesis in the rat. Anat Rec 182:367–376
Gottschalk C, Mylle M (1956) Micropuncture study of pressures in proximal tubules and peritubular capillaries of the rat kidney and their relation to ureteral and renal venous pressures. Am J Physiol185:430–439
Greger R, Lang F, Marchand G, Knox FG (1977) Site of renal phosphate reabsorption. Micropuncture and microinfusion study. Pflügers Arch 369:111–118
Haas JA, Berndt T, Knox FG (1978) Nephron heterogeneity of phosphate reabsorption. Am J Physiol 234:F287–F290
Jamison RL, Lacy FB (1971) Effect of saline infusion on superficial and juxtamedullary nephrons in the rat. Am J Physiol 221:690–697
Jamison RL, Roinel N, Rouffignac C de (1979) Urinary concentrating mechanism in the desert rodentPsammomys obesus. Am J Physiol 236:F448–F453
Kuntziger H, Amiel C, Gaudebout C (1972) Phosphate handling by the rat nephron during saline diuresis. Kidney Int 2:318–323
Lang F, Greger R, Marchand GR, Knox FG (1977) Stationary microperfusion study of phosphate reabsorption in proximal and distal nephron segments. Pflügers Arch 368:45–48
Le Grimellec C, Roinel N, Morel F (1974) Simultaneous Mg, Ca, P, K and Cl analysis in rat tubule fluid. IV. During acute phosphate plasma loading. Pflügers Arch 346:189–204
Morel F, Lechêne C (1965) Microinjections de sodium et d'inuline marqués dans les capillaires du rein de Hamster. II. Perméabilité au sodium des segments medullaires tubulaires. Nephron 2:219–228
Murayama Y, Morel F, Le Grimellec C (1972) Phosphate, calcium and magnesium transfers in proximal tubules and loops of Henle, as measured by single nephron microperfusion experiments in the rat. Pflügers Arch 333:1–16
Pastoriza-Munoz E, Colindres RE, Lassiter WE, Lechêne C (1978) Effect of parathyroid hormone on phosphate reabsorption in rat distal convolution. Am J Physiol 235:F321–F330
Poujeol P, Chabardes D, Roinel N, Rouffignac C de (1976) Influence of extracellular fluid volume expansion on magnesium, calcium and phosphate handling along the rat nephron. Pflügers Arch 365:203–211
Poujeol P, Corman B, Touvay C, Rouffignac C de (1977) Phosphate reabsorption in rat nephron terminal segments. Intrarenal heterogeneity and strain differences. Pflügers Arch. 371:39–49
Rocha A, Magaldi J, Kokko J (1977) Calcium and phosphate transport in isolated segments of rabbit Henle's loop. J Clin Invest 59:975–983
Rouffignac C de, Bonvalet JP (1970) Etude chez le rat des variations du débit individual de filtration glomérulaire des néphrons superficiels et profonds en fonction de l'apport sodé. Pflügers Arch 317:141–156
Rouffignac C de, Morel F, Moss N, Roinel N (1973) Micropuncture study of water and electrolyte movements along the loop of Henle in Psammomys with special reference to magnesium, calcium and phosphorus. Pflügers Arch 344:309–326
Rouffignac C de, Bankir L, Roinel N (1980) Etude de la fonction rénale d'un rongeur désertique saharien, le Goundi (Ctenodactylus vali). J Physiol (Paris) (in press)
Staum BB, Hamburger RJ, Goldberg M (1972) Tracer microinjection study of renal tubular phosphate reabsorption in the rat. J Clin Invest 51:2271–2276
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Poujeol, P., Jamison, R.L., de Rouffignac, C. et al. Phosphate reabsorption in juxtamedullary nephron terminal segments. Pflugers Arch. 387, 27–31 (1980). https://doi.org/10.1007/BF00580840
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00580840