Abstract
A radioimmunoassay for arginine-vasotocin (AVT), the antidiuretic principle in birds, was developed using the high cross-reactivity of AVT with an AVP antiserum raised in rabbits. This assay is specific for the measurement of AVT in serum of birds. The sensitivity and precision is such that serum AVT concentrations above 0.5 fmol/ml can be measured quantitatively.
A serum AVT concentration of 5.1±1.4 fmol/ml was found in normally hydrated, fresh water adapted ducks with a serum osmolality of 293.7±2.2 mosmol/kg. When the same animals were acutely hydrated, no or<0.5 fmol/ml AVT was present at an osmolality of 289.9±2.4 mosmol/kg. In salt water adapted ducks with a serum osmolality of 333.0±7.6 mosmol/kg, a serum AVT of 22.7±3.0 fmol/ml was found. When fresh water adapted ducks were hydrated and infused with different doses of AVT, a linear relationship between serum AVT and the amount infused was obtained. During AVT infusions, serum osmolality and urine flow rate were negatively correlated and urine osmolality was positively correlated with serum AVT.
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References
Beardwell CG (1971) Radioimmunoassay of arginine vasopressin in human plasma. J Clin Endocrinol Metab 33:254–260
Chard T (1973) The radioimmunoassay of oxytocin and vasopressin. J Endocr (Lond) 58:143–160
Cowley AW, Switzer SJ (1979) Evidence and quantification of the vasopressin arterial pressure control system in the dog. Circ Res
Deutsch H, Hammel HT, Simon E, Simon-Oppermann Ch (1979) Osmolality and volume factors in salt gland control of Pekin ducks after adaptation to chronic salt loading. J Comp Physiol 129:301–308
Dunn FL, Brennan TJ, Nelson AE, Robertson GL (1973) The role of blood osmolality and volume in regulating vasopressin secretion in the rat. J Clin Invest 52:3212–3219
Düsterdieck G, McElwee G (1971) Iodination of angiotensin II and purification of the labelled hormone. In: Kirkham KE, Hunter WM (eds) Radioimmunoassay methods. Churchill Livingstone, Edinburgh and London, pp 24–30
Ekins RP (1974) Radioimmunoassay and saturation analysis: Basic principles and theory. Br Med Bull 30:3–11
Ekins RP, Newman GB, O'Riordan JLH (1968) Theoretical aspects of “saturation” and radioimmunoassay. In: Hayes RL, Goswitz FA, Murphy BEP (eds) Radioisotopes in medicine: In vitro studies. Atomic Energy Commission, Oak Ridge, pp 59–100
Goodfriend TL, Levine L, Fasman GD (1964) Antibodies to bradykinin and angiotensin: a use of carbodiimides in immunology. Science 144:1344–1346
Hayward JN, Pavasuthipaisit K, Perez-Lopez FR, Sofroniew MV (1976) Radioimmunoassay of arginine vasopressin in rhesus monkey plasma. Endocrinology 98:975–981
Hunter WM (1971) The preparation and assessment of iodinated antigens. In: Kirkham KE, Hunter WM (eds) Radioimmunoassay methods. Churchill Livingstone, Edinburgh and London, pp 3–23
Hunter WM, Greenwood FC (1962) Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature 194:495–496
Husain MK, Fernado N, Shapiro M, Kagan A, Glick SM (1973) Radioimmunoassay of arginine vasopressin in human plasma. J Clin Endocrinol 37:616–625
Koike TI, Pryor LR, Neldon HL, Venable RS (1977) Effect of water deprivation of plasma radioimmunoassayable arginine vasotocin in conscious chickens (Gallus domesticus). Gen Comp Endocrinol 33:359–364
Möhring B, Möhring J (1975) Plasma ADH in normal Long-Evans rats and in Long-Evans rats heterozygous and homozygous for hypothalamic diabetes insipidus. Life Sci 17:1307–1314
Moses AM, Miller M (1974) Osmotic influences on the release of vasopressin. In: Knobil E, Sawyer WH (eds) Handbook of Physiology, Section 7: Endocrinology, Vol IV: the pituitary gland and its neuroendocrine control, Part 1, American Physiological Society, Washington, D.C., pp 225–255
Prusik Z, Sedlakova E, Barth T (1972) Isolation of Arg8-vasopressin from the neurophysin complex by free-flow continuous electrophoresis. Hoppe Seylers Z Physiol Chem 353:1837–1840
Robertson GL (1977) The regulation of vasopressin function in health and disease. Recent Prog Horm Res 33:333–374
Robertson GL, Athar S, Shelton RL (1977) Osmotic control of vasopressin function. In: Andreoli TE, Granthum JJ, Rector FC (eds) Disturbances in body fluid osmolality. American Physiological Society, Bethesda, pp 125–148
Robertson GL, Klein LA, Roth J, Gordon P (1970) Immunoassay of plasma vasopressin in man. Proc Natl Acad Sci 66:1298–1305
Rosenblom AA, Fisher DA (1974) Radioimmunoassay of arginine vasotocin. Endocrinology 95:1726–1732
Sawyer WH, Pang PKT (1975) Endocrine adaptation to osmotic requirements of the environment: endocrine factors in osmoregulation by lungfish and amphibians. Gen Comp Endocrinol 25:224–229
Yalow RS (1973) Radioimmunoassay methodology: application to problems of heterogeneity of peptide hormones. Pharmacol Rev 25:161–178
Yalow RS, Berson SA (1968) General principles of radioimmunoassay. In: Hayes RL, Goswitz FA, Murphy BEP (eds) Radioisotopes in medicine: In vitro studies. Atomic Energy Commission, Oak Ridge, pp 7–41
Zettner A (1973) Principles of competitive binding assays (saturation analysis). I. Equilibrium techniques. Clin Chem 19:699–705
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Möhring, J., Schoun, J., Simon-Oppermann, C. et al. Radioimmunoassay for arginine-vasotocin (AVT) in serum of Pekin ducks: AVT concentrations after adaptation to fresh water and salt water. Pflugers Arch. 387, 91–97 (1980). https://doi.org/10.1007/BF00584258
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DOI: https://doi.org/10.1007/BF00584258